Summary
Dopamine and somatostatin-14 (SRIF) were incubated with a membrane fraction of rat caudate-putamen (CP) tissue in an adenylate cyclase assay in order to examine the D-1-receptor coupled adenylate cyclase activity 5 days and 3 weeks after unilateral ablation of the left frontal and lateral cortex. Five days after decortication the ipsilateral basal and dopamine stimulated adenylate cyclase activity was increased by about 30% compared to that of the contralateral side. Three weeks after decortication no significant difference could be seen. On either side basal and dopamine stimulated adenylate cyclase activity was not significantly decreased compared to sham operated controls. Somatostatin (10-7 mol/l) reduced basal adenylate cyclase activity of the ipsilateral CP five days following lesioning and reduced the maximal stimulation induced by dopamine. The effects of somatostatin were most marked in the absence and at low concentrations of dopamine (10-7–10-6 mol/l). The effects of somatostatin in the lesioned CP were no longer apparent three weeks following surgery. These results do not favour a presynaptic localization of D-1-receptors on cortico-striate projection fibers and suggest that somatostatin is involved in the interaction of the cortico-striate and nigro-striatal projection systems and may play a role in the regulation of D-1-receptor linked adenylate cyclase.
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References
Beal MF, Martin JB (1983) Effects of lesions on somatostatin-like immunoreactivity in the rat striatum. Brain Res 226: 67–73
Bensadoun A, Weinstein D (1976) Assay of protein in the presence of interfering materials. Anal Biochem 70: 241–250
Carman JB, Cowan WM, Powell TPS, Webster KE (1965) A bilateral cortico-striate projection. J Neurol Neurosurg Psychiat 28: 71–77
Chneiweiss H, Glowinski J, Prémont J (1985) Vasoactive intestinal polypeptide receptors linked to an adenylate cyclase, and their relationship with biogenic amine- and somatostatin-sensitive adenylate cyclases on central neuronal and glial cells in primary cultures. J Neurochem 44: 779–786
Cotman CW, Forster A, Lanthorn T (1981) Glutamate as a neurotransmitter — an overview of glutamate as a neurotransmitter. J Neurochem 36: 413–422
Finklestein S, Campell A, Stoll AL, Baldessarini RJ, Stinus L, Paskevitch PA, Domesick VB (1983) Changes in cortical and subcortical levels of monoamines and their metabolites following unilateral ventrolateral cortical lesions in the rat. Brain Res 271: 279–288
Fonnum F, Storm-Mathisen J, Divac I (1981) Biochemical evidence for glutamate as neurotransmitter in cortico-striatal and cortico-thalamic fibres in rat brain. Neuroscience 6: 863–873
Hassler R, Haug P, Nitsch C, Kim JS, Paik K (1982) Effect of motor and premotor cortex ablation on concentrations of amino acids, monoamines, and acetylcholine and on the ultrastructure in rat striatum. A confirmation of glutamate as the specific cortico-striatal transmitter. J Neurochem 38: 1087–1098
Kebabian JWS, Petzold GL, Greengard P (1972) Dopamine sensitive adenylate cyclase activity in caudate nucleus of rat brain and its similary to the dopamine receptors. Proc Natl Acad Sci (Wash) 69: 2145–2149
Kim JS, Hassler R, Haug P, Paik KS (1977) Effect of frontal cortex ablation on striatal glutamic acid level in the rat. Brain Res 132: 370–374
Kornhuber J, Kornhuber E (1986) Presynaptic dopaminergic modulation of cortical input to the striatum. Life Sci 39: 669–674
Mishra RK, Marshall AM, Varmuza SL (1980) Supersensitivity in rat caudate nucleus: effects of 6-hydroxydopamine on the time course of dopamine receptor and cyclic AMP changes. Brain Res 200: 47–57
Moser A, Reavill C, Jenner P, Marsden CD, Cramer H (1986) Effects of somatostatin on dopamine sensitive adenylate cyclase activity in the caudate-putamen of the rat. Exp Brain Res 62: 567–571
Reibaud M, Blanc G, Studler JM, Glowinski J, Tassin JP (1984) Non-DA prefronto-cortical efferents modulate D-1-receptors in the nucleus accumbens. Brain Res 305: 43–50
Reisine T (1984) Somatostatin desensitization: loss of ability of somatostatin to inhibit cyclic AMP accumulation and adrenocorticotropin hormone release. J Pharmacol Exp Therap 229: 14–20
Reisine T, Zhan Y, Sekura RD (1983) Pertussis toxin blocks somatostatin's inhibition of stimulation cyclic AMP accumulation in anterior pituitary tumor cells. Biochem Biophys Res Commun 115: 794–799
Rodbell M, Lad PM, Nielsen TB, Cooper DMF, Schlegel W, Preston MS, Londos C, Kempner ES (1981) The structure of adenlyate cyclase systems. Adv Cyclic Nucleotide Res 14: 3–14
Roy C (1984) Inhibition by somatostatin of the vasopressin-stimulated adenylate cyclase in a kidney-derived line of cells grown in defined medium. FEBS Lett 169: 133–137
Salomon Y (1979) Adenylate cyclase assay. Adv Cyclic Nucleotide Res 10: 35–55
Sandberg M, Ward HK, Bradford HF (1985) Effect of corticostriate pathway lesion on the activities of enzymes involved in synthesis and metabolism of amino acid neurotransmitters in the striatum. J Neurochem 44: 42–47
Schwarcz R, Creese I, Coyle JT, Snyder SH (1978) Dopamine receptors localised on cerebral cortical afferents to rat corpus striatum. Nature (Lond) 271: 766–768
Theodorou A, Reavill C, Jenner P, Marsden CD (1981) Kainic acid lesions of striatum and decortication reduce specific 3H-sulpiride binding in rats, so D-2-receptors exist post-synaptically on cortico-striate afferents and striatal neurons. J Pharm Pharmacol 33: 439–444
Wise S, Jones EG (1977) Cells of origin and terminal distribution of descending projections of the rat somatic sensory cortex. J Comp Neurol 175: 129–157
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Moser, A., Reavill, C., Liebetrau, A. et al. Dopamine and somatostatin modulated adenylate cyclase activity in the rat caudate-putamen following unilateral cortical ablation. Exp Brain Res 68, 406–410 (1987). https://doi.org/10.1007/BF00248805
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DOI: https://doi.org/10.1007/BF00248805