Summary
The efferent projections of the five histaminergic neuronal subgroups in the tuberomammillary nucleus to the medial preoptic area (MPO) and inferior colliculus (IC) were examined by immunocytochemistry with antihistidine decarboxylase (HDC) antibodies combined with retrograde axonal tracing with Fast Blue (FB). The term “E groups” were used for the histaminergic neuronal subgroups. About 10% of the HDC-immunoreactive (HDCI) neurons were retrogradely labeled after FB injection into the MPO. The labeled neurons were not concentrated in any particular area, but were diffusely distributed bilaterally in all the subgroups. About two-thirds of the labeled neurons were observed on the side ipsilateral to the injection site and one-third on the contralateral side. The percentages of labeled neurons (double-labeled neurons/HDCI neurons) in the five subgroups were not significantly different with each other. The percentages in group E1 and E2 were particularly close, while that in group E4 resembled that in group E5. About 4% of the HDCI neurons were retrogradely labeled after the dye injections into the IC, and about half of the labeled neurons were detected on the ipsilateral side. The percentage of the double-labeled neurons in the five groups were not significantly different. Furthermore, those in E1 and E2, and in E4 and E5 were almost identical, respectively, to the situation following injection of FB into the MPO. These results indicate that each subgroup of histaminergic neurons in the tuberomammillary nucleus has similar efferent projections to the MPO and IC.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bleier R, Cohn P, Siggelcow IR (1979) A cytoarchitectonic atlas of the hypothalamus and hypothalamic third ventricle of the rat. In: Morgane PJ, Panksepp J (eds) Handbook of the hypothalamus, Vol I. Anatomy of the hypothalamus. Marcel Dekker, New York, pp 137–220
Dahlström A, Fuxe K (1964) Evidence for the existence of monoamine-containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brain stem neurons. Acta Physiol Scand 62:1–55
Ericson H, Watanabe T, Köhler C (1987) Morphological analysis of the tuberomammillary nucleus in the rat brain: delineation of subgroups with antibody against L-histidine decarboxylase as a marker. J Comp Neurol 263:1–24
Hökfelt T, Fuxe K, Goldstein M, Johansson O (1974) Immunohistochemical evidence for the existence of adrenaline neurons in the rat brain. Brain Res 66:235–251
Hayashi H, Takagi H, Takeda N, Kubota Y, Tohyama M, Watanabe T, Wada H (1984) Fine structure of histaminergic neurons in the caudal magnocellular nucleus of the rat as demonstrated by immunocytochemistry using histidine decarboxylase as a marker. J Comp Neurol 229:233–241
Inagaki N, Yamatodani A, Ando-Yamamoto M, Tohyama M, Watanabe T, Wada H (1988) Organization of histaminergic fibers in the rat brain. J Comp Neurol 327:283–300
Jaeger CB, Ruggiero DA, Albert VR, Joh TH, Reis DJ (1984) Immunocytochemical localization of aromatic-L-aminoacid decarboxylase. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, Vol. 2. Classical transmitters in the CNS, Part I. Elsevier, Amsterdam, pp 387–408
Köhler C, Swanson LW, Haglund L, Wu J-Y (1985) The cytoarchitecture, histochemistry and projections of the tuberomammillary nucleus in the rat. Neuroscience 16:85–110
Panula P, Yang H-Y T, Costa E (1984) Histamine-containing neurons in the rat hypothalamus. Proc Natl Acad Sci USA 81:2572–2576
Panula P, U Pirvola, Auvinen S, Airakisinen MS (1989) Histamineimmunoreactive nerve fibers in the rat brain. Neuroscience 28:585–610
Reiner PB, Semba K, Fibiger HC, McGeer EG (1988) Ontogeny of histidine-decarboxylase-immunoreactive neurons in the tuberomammillary nucleus of the rat hypothalamus: time of origin and development of transmitter phenotype. J Comp Neurol 276:304–311
Simerly RB, Swanson LW, Gorski RA (1984) The cells of origin of a sexually dimorphic serotonergic input to the medial preoptic nucleus of the rat. Brain Res 324:185–189
Staines WA, Yamamoto T, Daddona PE, Nagy JI (1986) Neuronal colocalization of adenosine deaminase, monoamine oxidase, galanin and 5-hydroxytryptophan uptake in the tuberomammillary nucleus of the rat. Brain Res Bull 17:351–365
Staines WA, Daddona PE, Nagy JI (1987) The organization and hypothalamic projections of the tuberomammillary nucleus in the rat: an immunohistochemical study of adenosine deaminasepositive neurons and fibers. Neuroscience 23:571–596
Steinbusch HWM, Mulder AH (1984) Immunohistochemical localization of histamine in neurons and mast cells in the rat brain. In: Björklund A, Hökfelt T, Kuhar MJ (eds) Handbook of chemical neuroanatomy, Vol 3. Classical transmitter receptors in the CNS, Part II. Elsevier, Amsterdam, pp 126–140
Steinbusch HWM, Sauren Y, Groenewegen H, Watanabe T, Mulder AH (1986) Histaminergic projections from the premammillary and posterior hypothalamic region to the caudate-putamen complex in the rat. Brain Res 368:289–393
Taguchi Y, Watanabe T, Kubota H, Hayashi H, Wada H (1984) Purification of histidine decarboxylase from the liver of fetal rats and its immunochemical and immunohistochemical characterization. J Biol Chem 259:5214–5221
Takeda N, Inagaki S, Taguchi Y, Tohyama M, Watanabe T, Wada H (1984) Origins of histamine-containing fibers in the cerebral cortex of rats studied by immunohistochemistry with histidine decarboxylase as a marker and transection. Brain Res 323:55–63
Wahlestedt C, Skagerberg G, Håkanson R, Sundler F, Wada H, Watanabe T (1985) Spinal projections of hypothalamic histidine decarboxylase-immunoreactive neurons. Agents Actions 16:231–233
Watanabe T, Taguchi Y, Shiosaka S, Tanaka J, Kubota H, Terano Y, Tohyama M, Wada H (1984) Distribution of the histaminergic neuron system in the central nervous system of rats: a fluorescent immunohistochemical analysis with histidine decarboxylase as a marker. Brain Res 295:13–25
Wouterlood FG, Steinbusch HWM, Luiten PGM, Bol JGJM (1987) Projection from the prefrontal cortex to histaminergic cell groups in the posterior hypothalamic region of the rat: anterograde tracing with Phaseolus vulgaris-leucoagglutinin (PHA-L) combined with immunocytochemistry of histidine decarboxylase. Brain Res 406:330–335
Wouterlood FG, Gaykema RPA (1988) Innervation of histaminergic neurons in the posterior hypothalamic region by medial preoptic neurons: anterograde tracing with Phaseolus vulgaris- leucoagglutinin combined with immunocytochemistry of histidine decarboxylase in the rat. Brain Res 455:170–176
Wouterlood FG, Gaykema RPA, Steinbusch HWM, Watanabe T, Wada H (1988) The connections between the septum-diagonal band complex and histaminergic neurons in the posterior hypothalamus of the rat: anterograde tracing with Phaseolus vulgaris-leucoagglutinin combined with immunocytochemistry of histidine decarboxylase. Neuroscience 26:827–845
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Inagaki, N., Toda, K., Taniuchi, I. et al. An analysis of histaminergic efferents of the tuberomammillary nucleus to the medial preoptic area and inferior colliculus of the rat. Exp Brain Res 80, 374–380 (1990). https://doi.org/10.1007/BF00228164
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00228164