Skip to main content
SpringerLink
Log in

Gastric afferents to the paraventricular nucleus in the rat

  • Published:
Experimental Brain Research Aims and scope Submit manuscript

Summary

Extracellular recordings were made from vasopressin (AVP) and oxytocin (OXT)-secreting cells in the paraventricular nucleus (PVN) of the hypothalamus in rats anesthetized with urethane-chloralose to determine the effects of electrical stimulation of vagal gastric nerves and gastric distension on their activity. Electrical stimulation of gastric branches of the vagus nerves inhibited 5 and excited 10 of 32 phasically firing neurosecretory cells. Approximately one third of the phasically firing neuro-secretory cells (9 out of 29 cells) were transiently inhibited by gastric distension; an effect which was completely abolished by bilateral cervical vagotomy. In contrast, gastric nerve stimulation excited 45 of 72 non-phasically firing paraventricular cells. Thirteen of 77 non-phasically firing cells tested were excited by gastric distension. We conclude that there are some sensory afferent inputs originating from gastric receptors and transmitted by gastric vagal afferents which inhibit the activity of AVP- secreting neurons in the PVN although other inputs excite the cells. Similar inputs also excite some of the putative OXT-secreting neurons in the PVN.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anand BK, Pillai RV (1967) Activity of single neurones in the hypothalamic feeding centres: effect of gastric distension. J Physiol (Lond) 192: 63–77

    Google Scholar 

  • Arnauld E, du Pont J (1982) Vasopressin release and firing of supraoptic neurosecretory neurones during drinking in the dehydrated monkey. Pflügers Arch 394: 195–201

    Google Scholar 

  • Banks D, Harris MC (1987) Activation within dorsal medullary nuclei following stimulation in the hypothalamic paraventricular nucleus in rats. Pflügers Arch 408: 619–627

    Google Scholar 

  • Barber WD, Burks TF (1983) Brain stem response to phasic gastric distension. Am J Physiol 245: G242-G248

    Google Scholar 

  • Barker JL, Crayton JW, Nicoll RA (1971) Antidromic and orthodromic responses of paraventricular and supraoptic neurosecretory cells. Brain Res 33: 353–366

    Google Scholar 

  • Blair-West JR, Gibson AP, Woods RL, Brook AH (1985) Acute reduction of plasma vasopressin levels by rehydration in sheep. Am J Physiol 248: R68-R71

    Google Scholar 

  • Blair-West JR, Gibson AP, Sheather SJ, Woods RL, Brook AH (1987) Vasopressin release in sheep following various degrees of rehydration. Am J Physiol 253: R640-R645

    Google Scholar 

  • Calaresu FR, Ciriello J (1980) Projections to the hypothalamus from buffer nerves and nucleus tractus solitarius in the cat. Am J Physiol 239: R130-R136

    Google Scholar 

  • Davison JS, Clarke GD (1988) Mechanical properties and sensitivity to CCK of vagal gastric slowly adapting mechanoreceptors. Am J Physiol 255: G55-G61

    Google Scholar 

  • Dreifuss JJ, Ruf KB (1972) A transpharyngeal approach to the rat hypothalamus. In: Kerkut GA (ed) Experiments in physiology and biochemistry, Vol. 5 Academic Press, London, pp 213–228

    Google Scholar 

  • Geelen G, Keil LC, Kravik SE, Wade CE, Thrasher TN, Barnes PR, Pyka G, Nesvig C, Greenleaf JE (1984) Inhibition of plasma vasopressin after drinking in dehydrated humans. Am J Physiol 247: R968-R971

    Google Scholar 

  • Gonzalez MF, Sharp FR, Deutsch JA (1986) Gastric distention increases [14C]2-deoxyglucose uptake in the rat nucleus tractus solitarius. Brain Res 369: 395–399

    Google Scholar 

  • Harding R, Leek BF (1973) Central projections of gastric afferent vagal inputs. J Physiol (Lond) 228: 73–90

    Google Scholar 

  • Harris MC (1979) Effects of chemoreceptor and baroreceptor stimulation on the discharge of hypothalamic supraoptic neurones in rats. J Endocrinol 82: 115–125

    Google Scholar 

  • Hawthorn J, Andrews PLR, Ang VTY, Jenkins JS (1988) Differential release of vasopressin and oxytocin in response to abdominal vagal afferent stimulation or apomorphine in the ferret. Brain Res 438: 193–198

    Google Scholar 

  • Kannan H, Yamashita H (1983) Electrophysiological study of paraventricular nucleus neurons projecting to the dorsomedial medulla and their response to baroreceptor stimulation in rats. Brain Res 279: 31–40

    Google Scholar 

  • Kannan H, Osaka T, Kasai M, Okuya S, Yamashita H (1986) Electrophysiological properties of neurons in the caudal ventrolateral medulla projecting to the paraventricular nucleus of the hypothalamus in rats. Brain Res 376: 342–350

    Google Scholar 

  • Koizumi K, Yamashita H (1978) Influence of atrial stretch receptors on hypothalamic neurosecretory neurones. J Physiol 285: 341–358

    Google Scholar 

  • König JFR, Klippel RA (1963) The rat brain: a stereotaxic atlas of the forebrain and lower parts of the brain stem. Williams and Wilkins, Baltimore

    Google Scholar 

  • Leek BF (1977) Abdominal and pelvic visceral receptors. Brain Med Bull 33(2): 163–168

    Google Scholar 

  • McCann MJ, Verbalis JG, Stricker EM (1989) LiCl and CCK inhibit gastric emptying and feeding and stimulate OT secretion in rats. Am J Physiol 256: R463-R468

    Google Scholar 

  • Nicolaïdis S (1969) Early systemic responses to orogastric stimulation in the regulation of food and water balance: functional and electrophysiological data. Ann NY Acad Sci 157: 1176–1203

    Google Scholar 

  • Nosaka S (1984) Solitary nucleus neurons transmitting vagal visceral input to the forebrain via a direct pathway in rats. Exp Neurol 85: 493–505

    Google Scholar 

  • Poulain DA, Wakerley JB, Dyball REJ (1977) Electrophysiological differentiation of oxytocin- and vasopressin-secreting neurones. Proc R Soc Lond [Biol] 196: 367–384

    Google Scholar 

  • Rogers RC, Hermann GE (1986) Hypothalamic paraventricular nucleus stimulation-induced gastric acid secretion and bradycardia suppressed by oxytocin antagonist. Peptides 7: 695–700

    Google Scholar 

  • Renaud LP, Tang M, McCann MJ, Stricker EM, Verbalis JG (1987) Cholecystokinin and gastric distension activate oxytocinergic cells in rat hypothalamus. Am J Physiol 253: R661-R665

    Google Scholar 

  • Sakaguchi T, Tamaki M, Akaishi T, Miyaoka Y (1989) Responses in discharge of vasopressinergic neurons in the hypothalamic paraventricular nucleus elicited by water application to the pharyngolaryngeal regions in the rat. Chem Senses 14(3): 327–333

    Google Scholar 

  • Salata RA, Verbalis JG, Robinson AG (1987) Cold water stimulation of oropharyngeal receptors in man inhibits release of vasopressin. J Clin Endocrinol Metab 65(3): 561–567

    Google Scholar 

  • Swanson LW, Sawchenko PE (1983) Hypothalamic integration: organization of the paraventricular and supraoptic nuclei. Ann Rev Neurosci 6: 269–324

    Google Scholar 

  • Thompson CJ, Burd JM, Baylis PH (1987) Acute suppression of plasma vasopressin and thirst after drinking in hypernatremic humans. Am J Physiol 252: R1138-R1142

    Google Scholar 

  • Thrasher TN, Nistal-Herrera JF, Keil LC, Ramsay DJ (1981) Satiety and inhibition of vasopressin secretion after drinking in dehydrated dogs. Am J Physiol 240: E394-E401

    Google Scholar 

  • Thrasher TN, Keil LC, Ramsay DJ (1987) Drinking, oropharyngeal signals, and inhibition of vasopressin secretion in dogs. Am J Physiol 253: R509-R515

    Google Scholar 

  • Ueta Y, Kannan H, Yamashita H (1986) Inhibition of vasopressin secreting neurons of the paraventricular nucleus by gastric distension in rats. J Univ Occup Environ Health 8(4): 429–435 (in Japanese)

    Google Scholar 

  • Ueta Y, Kannan H, Yamashita H (1988) Paraventricular neuro-secretory neurons: synaptic inputs from the stomach in rats. 8th Int Congr Endocrinol Sat Symp “Posterior pituitary hormones”, Abstr 21

  • Verbalis JG, McCann MJ, McHale CM, Stricker EM (1986) Oxytocin secretion in response to cholecystokinin and food: differentiation of nausea from satiety. Science 232: 1417–1419

    Google Scholar 

  • Verbalis JG, Richardson DW, Stricker EM (1987) Vasopressin release in response to nausea-producing agents and cholecystokinin in monkeys. Am J Physiol 252: R749-R753

    Google Scholar 

  • Yamashita H, Inenaga K, Koizumi K (1984) Possible projections from regions of paraventricular and supraoptic nuclei to the spinal cord: electrophysiological studies. Brain Res 296: 373–378

    Google Scholar 

  • Yamashita H, Kannan H, Inenaga K, Koizumi K (1983a) Role of neurones in the supraoptic and paraventricular nuclei in cardiovascular control. Prog Brain Res 60: 459–468

    Google Scholar 

  • Yamashita H, Inenaga K, Kawata M, Sano Y (1983b) Phasically firing neurons in the supraoptic nucleus of the rat hypothalamus: immunocytochemical and electrophysiological studies. Neurosci Lett 37: 87–92

    Google Scholar 

  • Yamashita H, Kannan H, Ueta Y (1989) Involvement of caudal ventrolateral medulla neurons in mediating visceroreceptive information to the hypothalamic paraventricular nucleus. Prog Brain Res 81: 293–302

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, University of Occupational and Environmental Health, School of Medicine, Iseigaoka, Yahatanishi-ku, 807, Kitakyushu, Japan

    Y. Ueta, H. Kannan & H. Yamashita

Authors
  1. Y. Ueta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Kannan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ueta, Y., Kannan, H. & Yamashita, H. Gastric afferents to the paraventricular nucleus in the rat. Exp Brain Res 84, 487–494 (1991). https://doi.org/10.1007/BF00230960

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00230960

Key words

Search

Navigation