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GABAergic boutons establish synaptic contacts with the soma and dendrites of cuneothalamic relay neurons in the rat cuneate nucleus

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Abstract

This study investigates the synaptic relation between γ-aminobutyric acid-immunoreactive (GABA-IR) and cuneothalamic relay neurons (CTNs) in the rat cuneate nucleus. Retrograde transport of wheat germ agglutinin conjugated with horseradish peroxidase complex (WGA-HRP) was used to label CTNs while anti-GABA immunogold serum was used for the detection of GABA-IR boutons associated with CTNs. With these procedures, immunogold-labelled GABA-IR boutons were found to form axosomatic, axodendritic and axospinous synapses with the WGA-HRP-labelled but immunonegative CTNs. Quantitative estimation showed that the mean ratios of GABA-IR to GABA-immunonegative boutons making synaptic contacts with somata, proximal dendrites, and distal dendrites were 47.9%, 49.1% and 34.7%, respectively. Statistical analysis showed that the incidence of GABA-IR boutons on the somata and proximal dendrites of CTNs was significantly higher than on the distal dendrites. Our results indicate that GABA is the primary inhibitory neurotransmitter in the cuneate nucleus, thereby emphasizing the importance of postsynaptic inhibition on cuneothalamic relay neurons.

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine, National Taiwan University, 1, Sec. 1, Jen Ai Road, Taipei, Taiwan 10018, Republic of China

    J. H. Lue, J. Y. Shieh, C. Y Wen, K. N. Chen & S. A. Chan

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  1. J. H. Lue
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  2. J. Y. Shieh
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  3. C. Y Wen
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  4. K. N. Chen
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  5. S. A. Chan
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Lue, J.H., Shieh, J.Y., Wen, C.Y. et al. GABAergic boutons establish synaptic contacts with the soma and dendrites of cuneothalamic relay neurons in the rat cuneate nucleus. Exp Brain Res 98, 13–20 (1994). https://doi.org/10.1007/BF00229104

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  • DOI: https://doi.org/10.1007/BF00229104

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