Abstract
The isolation of biologically important low molecular weight organic acids from organically enriched sediments in Loch Eil, Scotland, was carried out by extraction of pore water with acidified ethyl acetate. High concentrations of acetic acid, up to 1.8 mg g-1 dry weight of sediment were found at Station E-24. Propionate, butyrate, valerate, lactate and traces of succinate were also found. Succinate was present in significant amounts, 42.2 μg g-1 dry weight of sediment at Station E-70, which received a higher input of organic matter than E-24. Both propionate and succinate were absent from a control station in the Lynn of Lorne where the sediment was low in carbon compared with Loch Eil. In experimental tanks, acetate levels increased as the input of organic carbon (as cellulose) was increased up to a load level of 1.5 g m-2 day-1. Above this, acetate decreased and succinate appeared. Succinate was not detected in low-loaded tanks. Experiments with sieved mud showed a vertical distribution of the different acids with depth. Lactate and succinate reached highest concentration in the 0 to 3 cm layer, acetate at 3 to 6 cm and propionate at 6 to 9 cm. The results are discussed in relation to the role of these acids as food sources and as indicators of biochemical pathways taking place in sediments with different carbon input levels.
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Literature Cited
Blake, D. and J.W. Leftley: Studies on anaerobic nitrogen fixation in the sediments of two Scottish sea lochs. In: Biology of benthic organisms, pp 79–84. Ed. by B.F. Keegan, P.O. Ceidigh and P.J.S. Boaden. Oxford: Pergamon Press 1977
Bordovskly, O.K.: Transformation of organic matter in bottom sediments and its early diagenesis. Mar. Geol. 3, 83–114 (1965)
Cahet, G.: Aspects chemostrophiques en sédiments lagunaires. Cas du soufre. Vie Milieu 21, 1–36 (1970)
—: Relations carbone-soufre dans les sédiments marins de la région de Banyuls-sur-Mer. Vie Milieu 22, 39–60 (1971)
—: Transfert d'energie en milieu sedimentaire. Cas des sulfatoréducteurs. 1. Les acides organiques, distribution. Vie Milieu 24, 235–256 (1974)
—: Biogéochimie du carbone et du soufre en sédiments littoraux méditerranéens (Golfe du Lion): modèles biopédologiques. Vie Milieu 26, 1–26 (1976)
Cappenberg, T.E.: Inter-relations between sulphate reducing and methane producing bacteria in bottom deposits of a fresh water lake. I. Field observations. Antonie van Leeuwenhoek 40, 285–295 (1971)
Cappenberg, T.E.: Inter-relations between sulphate reducing and methane producing bacteria in bottom deposits of a fresh water lake. II. Inhibition experiments. Antonie van Leeuwenhoek 40, 297–306 (1974)
—: Relationships between sulphate reducing and methane producing bacteria. Pl. Soil. 43, 125–139 (1975)
— and R.A. Prins: Inter-relations between sulphate reducing and methane producing bacteria in bottom deposits of a fresh water lake. III. Experiments with C14-labelled substrates. Antonie van Leeuwenhoek 40, 457–469 (1974)
Craib, J.S.: A sampler for taking short undisturbed marine cores. J. Cons. perm. int. Explor. Mer 30, 34–39 (1965)
Hautala, E. and M.L. Weaver: Separation and qualitative determination of lactic, pyruvic, fumaric, succinic, malic and citric acid by gas chromatography. Analyt. Biochem. 30, 32–39 (1969)
Hoering, T.C.: Organic acids from the oxidation of recent sediments. Rep. Carnegie Inst. 1966/67, 515–527 (1967)
Iwase, H. and T. Sugiyoma: Gas chromatographic determination of organic acids. II. Extraction conditions of the acids in aqueous solution. Japan Analyst 24, 665–672 (1975)
Mandelstam, H. and K. McQuillen: Biochemistry of bacterial growth, 582 pp. Oxford: Blackwells 1973
Morris, R.J. and G. Eglinton: Fate and recycling of carbon compounds. Mar. Chem. 5, 559–572 (1977)
Mountfoot, D.O. and M. Robertson: Origins of fermentation products formed during growth of Bacteroides ruminicola on glucose. J. gen. Microbiol. 106, 353–360 (1978)
Pearson, T.H.: The benthic ecology of Loch Linnhe and Loch Eil, a sea loch system on the West Coast of Scotland. I. The physical environment and distribution of macrobenthic fauna. J. exp. mar. Biol. Ecol. 5, 1–34 (1970)
—: The benthic ecology of Loch Linnhe and Loch Eil, a sea loch system on the West Coast of Scotland. III. The effect on the benthic fauna of the introduction of pulp mill effluent. J. exp. mar. Biol. Ecol. 6, 211–233 (1971)
—: The benthic ecology of Loch Linnhe and Loch Eil, a sea loch system on the West Coast of Scotland. IV. Changes in the benthic fauna attributable to organic enrichment. J. exp. mar. Biol. Ecol. 20, 1–41 (1975)
— and R. Rosenberg: A comparative study of the effects on the marine environment of wastes from cellulose industries in Scotland and Sweden. Ambio 5, 77–79 (1976)
——: Macrobenthic succession in relation to organic enrichment and pollution of the marine environment. Oceanogr. mar. Biol. A. Rev. 16, 229–311 (1978)
— and S.O. Stanley: The benthic ecology of some Shetland voes. In: Biology of benthic organisms, pp 79–84. Ed. by B.F. Keegan, P.O. Ceidigh and P.J.S. Boaden. Oxford: Pergamon Press 1977
Riddle, U.M.: Determination of traces of organic acids in small samples. Gas liquid chromatography of the total concentration. Analyt. Chem. 35, 853–859 (1963)
Robbins, J.A. and J. Gustinis: A squeezer for efficient extraction of pore water from small volumes of marine sediment. Limnol. Oceanogr. 21, 905–908 (1976)
Siegel, W.H., T. Donohue and R.W. Bernlohr: Determination of pools of tricarboxylic acid cycle and related acids in bacteria. Appl. envirl Microbiol. 34, 512–517 (1977)
Southward, A.J. and E.C. Southward: Observations on the role of dissolved organic compounds in the nutrition of benthic invertebrates. III. Uptake in relation to organic content of the habitat. Sarsia 50, 29–46 (1972)
Stanley, S.O., T.H. Pearson and C.M. Brown: Marine microbial ecosystems and the degradation of organic pollutants. In: The oil industry and microbial ecosystems, pp 60–79. Ed. by K.W.A. Chater and H. Sommerville. London: Heyden 1978
Stephens, G.C.: Dissolved organic matter as a potential source of nutrition for marine organisms. Am. Zool. 8, 96–100 (1968)
Testerman, U.K.: Accumulation of free fatty acids from sea water by marine invertebrates. Biol. Bull. mar. biol. Lab., Woods Hole 142, 160–177 (1972)
Vance, I.: Bacterial degradation of cellulose in marine sediments, Ph.D. thesis, University of Dundee 1977
Whittle, K.J.: Marine organisms and their contribution to organic matter in the ocean. Mar. Chem. 5, 381–441 (1977)
Wood, L.W. and K.E. Chua: Glucose flux at the sediment water interface at Toronto Harbour, Lake Ontario, with reference to pollution stress. Can. J. Microbiol. 19, 413–420 (1973)
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Communicated by J. Mauchline, Oban
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Miller, D., Brown, C.M., Pearson, T.H. et al. Some biologically important low molecular weight organic acids in the sediments of Loch Eil. Mar. Biol. 50, 375–383 (1979). https://doi.org/10.1007/BF00387015
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DOI: https://doi.org/10.1007/BF00387015