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Quantitation of phenobarbital and its main metabolites in human urine

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Summary

A method for the quantitative determination of phenobarbital and free and conjugatedp-hydroxyphenobarbital in urine samples is described. The method includes initial extraction, purification on a small chromatographic column and finally determination by gas chromatography. The barbituric acids are methylated by trimethylanilinium hydroxide which serves as a “flash heater” methylating agent. The conjugate ofp-hydroxyphenobarbital, which appears to be a glucuronide, is hydrolysed with hydrochloric acid.

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Authors and Affiliations

  1. The Karolinska Pharmacy, Stockholm, Sweden

    N. Kållberg & S. Agurell

  2. Dept. of Organic Chemistry, Faculty of Pharmacy, University of Uppsala, Stockholm, Sweden

    Ö. Ericsson

  3. Dept. of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden

    E. Bucht, B. Jalling & L. O. Boréus

Authors
  1. N. Kållberg
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  2. S. Agurell
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  3. Ö. Ericsson
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  4. E. Bucht
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  5. B. Jalling
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  6. L. O. Boréus
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Kållberg, N., Agurell, S., Ericsson, Ö. et al. Quantitation of phenobarbital and its main metabolites in human urine. Eur J Clin Pharmacol 9, 161–168 (1975). https://doi.org/10.1007/BF00614013

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  • DOI: https://doi.org/10.1007/BF00614013

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