Summary
The addition of the dissaccharides maltose (10, 20, 30 mM) and sucrose (30, 60 mM) to Bretschneider's organ protective HTK solution was evaluated to improve renal protection by an enhanced glycolytic energy supply. Canine kidneys were perfused for 8 min with either HTK solution or HTK solution containing additional disaccharides. After nephrectomy the kidneys were incubated at 25°C and metabolic parameters were determined at regular intervals. Maltose and sucrose are slowly cleaved during renal ischemia but maltose distinctly faster than sucrose. Maltose increases intraischemic ATP supply. However, 30 mM maltose was no better than 10 mM. 60 mM sucrose was about as effective for glycolysis as 10 mM maltose. However, possibly due to fructose release there was an accelerated decrease of adenine nucleotides with sucrose. Although fructose enters glycolysis it seems to have negative side-effects. Hence, probably neither sucrose nor fructose are appropriate for renal substrate supply during ischemia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andrews PM, Coffey AK (1982) Factors that improve the preservation of nephron morphology during cold storage. Lab Invest 46:100–120
Bastin J, Cambon N, Thompson M, Lowry OH, Burch HB (1987) Change in energy reserves in different segments of the nephron during brief ischemia. Kidney Int 31:1239–1247
Bergmeyer HU, Bernt E, Schraidt F, Stork H (1974) D-Glucose, Bestimmung mit Hexokinase und Glucose-6-phosphat-Dehydrogenase. In: Bergmeyer HU (ed) Methoden der Enzymatischen Analyse. Verlag Chemie, Weinheim, pp 1241–1259
Brazy PC, Mandel LJ, Gullans SR, Soltoff SP (1984) Interactions between phosphate and oxidative metabolism in proximal renal tubules. Am J Physiol 247:575–581
Bretschneider HJ, Gebhard MM, Preusse CJ (1981) Amelioration of myocardial protection by improvement of capacity and effectiveness of anaerobic glycolysis. In: Isselhard W (ed) Myocardial protection for cardiac surgery. Pharmazeutische Verlagsgesellschaft. München, pp 63–71
Bretschneider HJ, Helmchen U, Kehrer G (1988) Nierenprotektion. Klin Wochenschr 66:817–827
Burch HB, Choi S, Dence CN, Alvey TR, Cole BR, Lowry OH (1980) Metabolic effects of large fructose loads in different parts of the rat nephron. J Biol Chem 255:8239–8244
Fischer JH, Knupfer P, Beyer M (1985) Flush solution 2, a new concept for one-to-three-day hypothermic renal storage preservation. Transplantation 39:122–126
Gutmann J, Wahlefeld AW (1974) L-(+)-Lactat, Bestimmung mit Lactat-Dehydrogenase und NAD. In: Bergmeyer HU (ed) Methoden der Enzymatischen Analyse. Verlag Chemie, Weinheim, pp 1510–1514
Hers HG, Van Schaftingen E (1982) Fructose 2,6-bisphosphate 2 years after its discovery. Biochem J 206:1–12
Iles RA, Griffith JR, Stevens AN, Gadian DG, Porteous R (1980) Effects of fructose on the energy metabolism and acid-base status of the perfused starved-rat liver. Biochem J 192:191–202
Kallerhoff M, Hölscher M, Kehrer G, Kläß G, Bretschneider HJ (1985) Effects of preservation conditions and temperature on tissue acidification in canine kidneys. Transplantation 39:485–489
Kehrer G, Blech M, Gebhard MM, Kallerhoff M, Siekmann W, Helmchen U, Bretschneider HJ (1985) Günstige Effekte einer Glucose-Prämedikation auf den anaeroben Energieumsatz der Hundeniere bei Protektion mit einer histidingepufferten Lösung im Vergleich zu einer Osmofundin-Prämedikation. In: Harzmann R et al. (eds) Experimentelle Urologie, Springer, Berlin Heidelberg New York, pp 172–179
Kehrer G, Kallerhoff M, Probst R, Siekmann W, Blech M, Bretschneider HJ, Helmchen U (1985) Construction and experimental application of a catheter for selective arterial kidney perfusion in situ. Urol Res 13:85–89
Kehrer G, Blech M, Kallerhoff M, Kleinert H, Langheinrich M, Bretschneider HJ (in preparation) Glucose content and efficiency of glycolysis in protected ischemic kidney of different species.
Kübler W, Spieckermann PG (1970) Regulation of glycolysis in the ischemic and the anoxic myocardium. J Mol Cell Cardiol 1:351–377
Miller TB (1978) Cyclic AMP-mediated activation of hepatic glycogenolysis by fructose. Biochim Biophys Acta 540:151–161
Preusse CJ, Gebhard MM, Bretschneider HJ (1982) Interstitial pH value in the myocardium as indicator of ischemic stress of cardioplegically arrested hearts. Basic Res Cardiol 77:372–387
Price RG (1982) Urinary enzymes, nephrotoxicity and renal disease. Toxicology 23:99–134
Regan JJ, Doorneweerd DD, Gilboe DP, Nuttall FQ (1980) Influence of fructose on the glycogen synthase and phosphorylase systems in rat liver. Metabolism 29:965–969
Sussman I, Erecinska M, Wilson DF (1980) Regulation of cellular energy metabolism. The Crabtree effect. Biochim Biophys Acta 591:209–23
Van de Werve G, Hers HG (1979) Mechanism of activation of glycogen phosphorylase by fructose in the liver. Biochem J 178:119–126
Venkatachalam MA, Patel YJ, Kreisberg JI, Weinberg JM (1988) Energy thresholds that determine membrane integrity and injury in a renal epithelial cell line (LLC-PK1). J Clin Invest 81:745–758
Woods HF, Eggleston LV, Krebs HA (1970) The cause of hepatic accumulation of fructose 1-phosphate on fructose loading. Biochem J 119:501–510
Author information
Authors and Affiliations
Additional information
Supported by the Deutsche Forschungsgemeinschaft, SFB 330-Organ Protection, Göttingen, Federal Republic of Germany
Rights and permissions
About this article
Cite this article
Kehrer, G., Blech, M., Kallerhoff, M. et al. Intraischemic metabolic effects of different disaccharides on protected canine kidneys. Urol. Res. 17, 371–376 (1989). https://doi.org/10.1007/BF00510529
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00510529