Summary
The permanent epithelial cell-line EB 33 was developed from a human prostatic carcinoma. First attempts were made to characterize this strain by functional, morphological and kinetic parameters. The doubling time was found to be quite slow and to remain constant at 50.0 h during the exponential phase of growth over many passages. High acid phosphatase activity in the cytoplasma was found by histochemical means in comparison to HeLa cells. Electron microscopic studies suggested the epithelial origin of the cell-line. The karyotype was near triploid. Successful heterotransplantation into “nude mice” was achieved reproducibly. Histological examination of the heterotransplants revealed solid epithelial tumors with a rapid rate of growth. The findings reported suggest the prostatic epithelial origin of the cell-line EB 33. Their origin from the carcinomatous part of the explanted tissue remains still unproven. Future aspects for the development of an experimental model for human prostatic carcinoma are discussed.
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This work is supported by the Deutsche Forschungsgemeinschaft, Grants Nr. Schr 113/1-7.
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Okada, K., Schröder, F.H. Human prostatic carcinoma in cell culture: Preliminary report on the development and characterization of an epithelial cell line (EB 33). Urol. Res. 2, 111–121 (1974). https://doi.org/10.1007/BF00254727
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DOI: https://doi.org/10.1007/BF00254727