Abstract
The 17 base pair operator O R 3 oligonucleotide, which is the preferential binding site for the Cro repressor of phage λ, was studied by two-dimensional NMR spectroscopy. A sequential assignment procedure based on two-dimensional Nuclear Overhauser Effect (NOESY) and scalar coupling correlated (COSY) NMR spectroscopy, together with the knowledge of the oligodesoxynucleotide sequence, made it possible to assign the non-exhangeable base protons and the H1′ and the H2′-H2″ sugar protons of the O R 3 operator DNA. The pattern of the observed NOE connectivities is consistent with a right-handed helical DNA structure. The base and sugar proton assignments provide the necessary information for further studies of the O R 3 operator — Cro repressor interaction.
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Abbreviations
- COSY:
-
correlated spectroscopy
- FID:
-
free induction decay
- NOE:
-
nuclear Overhauser effect
- NOESY:
-
nuclear Overhauser effect spectroscopy
- RD:
-
relaxation delay
- TSP:
-
sodium 3-trimethylsilyl-(2,2,3,3-2H4)propionate
- EDTA:
-
sodium ethylendiamine tetraacetate
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Hahn, K.D., Buck, F., Rüterjans, H. et al. 1H NMR study of the interaction of bacteriophage λ Cro protein with the O R 3 operator. Eur Biophys J 12, 87–95 (1985). https://doi.org/10.1007/BF00260431
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DOI: https://doi.org/10.1007/BF00260431