Abstract
Diffusion of water within cross-linked tetragonal crystals of hen egg-white lysozyme has been measured and simulated on a computer using the X-ray structure of water-filled channels within the crystal lattice. Relative to the self-diffusion coefficient of bulk water molecules, the experimental diffusion coefficient of water within the crystal was found to be 13 times reduced in the (001) crystallographic plane and 5 times reduced in the [001] direction. Comparison of the experimental and computer simulated diffusion coefficients shows that steric limitations for water diffusion are mostly responsible for this reduction of the water diffusion in the crystal, with the self-diffusion coefficient of intracrystalline water reduced by no more than 30–40% as compared to that of bulk water.
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References
Anisimov AV, Dautova NR (1993) Self-diffusion of water absorbed on a cotton fibers. Studies by pulsed NMR method. Biophysics (Rus) 38:500–503
Blake CCF, Koenig DF, Mair GA, North ACT, Phillips DC, Sarma VR (1965) Structure of hen egg-white lysozyme. A three dimensional Fourier synthesis at 2 A resolution. Nature 206:757–761
Brick MT, Tsapjuck EA (1989) Ultrafiltration. Naukova Dumka, Kiev
Churaev NV (1990) Physical Chemistry of Mass Transfer in Porous Bodies. Khimia, Moscow
Dytnerskii JuI (1986) Baromembrane Processes. Khimia, Moscow
Gevorkian SG, Morozov VN (1983) Dependence of lysozyme hydration isotherm on packing the molecules in solid phase. Biofizika 28:944–948
Imoto T, Johnson LN, North ACT, Phyllips DC, Rupley JA (1972) Vertebrate lysozyme. In: Boyer PD (ed) The Enzymes, 3-d edn, vol 7. Academic Press, London, pp 665–868
Kisluk OP, Kachalova GS, Lanina NPh (1994) An algorithm to find channels and cavities within protein crystals. J Mol Graphics 12:305–307
Knapp EW, Muegge I (1993) Heterogeneous diffusion of water at protein surfaces: Application to BPTI. J Phys Chem 97:11339–11343
Kotischke K, Kimmich K, Rommel E, Parak F (1990) NMR study of diffusion in protein hydration shells. Prog Colloid Poly Sci 83:211–215
Lee B, Richards FM (1971) The interpretation of protein structures: Estimation of static accessibility. J Mol Biol 55:379–400
Mattews BW (1968) Solvent content of protein crystals. J Mol Biol 33:491–497
Merten U (1966) Transport properties of osmotic membranes. In: Merten U (ed) Desalination by reverse osmosis. MIT Press, Cambridge Massachusetts London, pp 15–54
Morozov VN, Morozova TYa (1981) Viscoelastic properties of protein crystals: Triclinic crystals of hen egg white lysozyme in different conditions. Biopolymers 20:454–467
Morozov VN,Morozova TYa (1983) Rigidity increase of lysozyme molecules upon inhibitors binding. Biofizika 28:742–747
Morozov VN, Morozova TYa (1989) Immobilized protein crystals as a new material in biotechnology. In: Proc. 5th Internat Conf on Chem Biotechnol of Biol. Activ Nat Products, vol 4. Varna, Bulgaria, pp 66–80
Morozov VN, Morozova TYa (1992) Mechanical detection of interaction of small specific ligands with proteins and DNA in crosslinked state. Anal Biochem 201:68–79
Morozov VN, Morozova TYa, Kachalova GS, Myachin ET (1988) Interpretation of water desorption isotherm of lysozyme. Int J Biol Macromol 10:329–336
Polnaszek CF, Bryant RG (1984a) Nitroxide radical-induced solvent proton relaxation: measurement of localized translational diffusion. J Chem Phys 81:4038–4045
Polnaszek CF, Bryant RG (1984b) Self-diffusion of water at the protein surface: A measurement. J Am Chem Soc 106:428–429
Rupley JA, Carreri G (1991) Protein hydration and function. Adv Prot Chem 41:37–172
Robinson PJ, Dunnill P, Lilly MD (1971) Porous glass as a solid support for immobilization or affinity chromatography of enzymes. Biochim Biophys Acta 242:659–661
Sirotin UL Shaskolskaya MP (1975) Fundamentals of Crystallophysics. Nauka, Moscow
Steinhoff HJ, Kramm B, Hess G, Owerdiick C, Redhardt A (1993) Rotational and translational water diffusion in the hemoglobin hydration shell: dielectric and proton nuclear relaxation measurements. Biophys J 65:1486–1495
St. Clair NL, Navia MA (1992) Cross-linked enzyme crystals as robust biocatalysts. J Am Chem Soc 114:7314–7316
Steinrauf LK (1959) Preliminary X-ray data for some new crystalline forms of β-lactoglobulin and hen egg-white lysozyme. Acta Crystallogr 12:77–79
Yaroshchuk AE, Mesheryakova EV (1989) Effect of pore distribution in a membrane on reverse osmosis. Khim and Technol Vody (Russ) 11:776–782
Zatsepina GN (1987) Physical Properties and Structure of Water. Moscow State University, Moscow, pp 154–155
Zawodzinski TA Jr, Neeman M, Sillarud LO (1991) Determination of water diffusion coefficients in perfluorosulfonate ionomeric membranes. J. Phys Chem 95:6040–6044
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Morozov, V.N., Kachalova, G.S., Evtodienko, V.U. et al. Permeability of lysozyme tetragonal crystals to water. Eur Biophys J 24, 93–98 (1995). https://doi.org/10.1007/BF00211404
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DOI: https://doi.org/10.1007/BF00211404