Abstract
A newly defined leukocyte antigen system, the secondary B-cell (SB) system, was shown to be linked to HLA. The SB marker has been investigated in lymphocyte donors presumed to be genetically homozygous for HLA-A through HLA-D/DR by virtue of descent from a first-cousin marriage and of phenotypic homozygosity for these HLA markers. Of 19 donors, 3 were found to be heterozygous for SB. Studies of the families of these three donors could not distinguish with certainty whether the heterozygosity resulted from SB/DR recombination or from “pseudohomozygosity” for HLA-A through -D/DR by inheritance of two genetically unrelated but similar haplotypes. However, our data favored the occurrence of SB/DR recombination with a meiotic distance perhaps as large as 3.3 cMorgan. Recombinations were identified which mapped SB between HLA-B and GL01. These studies demonstrate the usefulness of cells from first-cousin marriage offspring in mapping a polymorphic genetic system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kavathas, P., DeMars, R., Bach, F. H., and Shaw, S.: A new human histocompatibility gene defined using HLA-mutant cell lines. Nature 293: 747–749, 1981
Mawas, C., Charmot, D., Mercier, P., North, M. L., and Hauptmann, G.: A weak human MLR locus mapping at the right of a crossing-over between HLA-D, Bf and GLO. J. Immunogenet. 5: 383–395, 1978
Mawas, C., Charmot, D., and Mercier, P.: Split of HLA-D into two regions α and β by a recombination between HLA-D and GLO. I. Study in a family lymphocyte typing for determinants coded by the β region. Tissue Antigens 15: 458–466, 1980
Meera Khan, P. and Doppert, B. A.: Rapid detection of glyoxalase I(GLO) on cellulose acetate gel and the distribution of GLO variants in a Dutch population, Hum. Genet. 34: 53–56, 1976
Pawelec, G., Shaw, S., Schneider, M., Blaurock, M., Frauer, M., Brackertz, D., and Wernet, P.: Population studies of HLA-linked SB antigens and their relative importance in primary MLC-typing. Analysis of HLA-D homozygous typing cells and normal heterozygous population. Hum. Immunol. 5: 215–223, 1982
Shaw, S., Johnson, A. H., and Shearer, G. M.: Evidence for a new segregant series of B cell antigens that are encoded in the HLA-D region and that stimulate secondary allogeneic proliferative and cytotoxic response J. Exp. Med. 152: 565–589, 1980
Shaw, S., Kavathas, P., Pollack, M. S., Charmot, D., and Mawas, C.: Family studies define a new histocompatibility locus, SB, between HLA-DR and GLO. Nature 293: 745–747, 1981a
Shaw, S., Duquesnoy, R. J., and Smith, P. L.: Population studies of the HLA-linked SB antigens. Immunogenetics 14: 153–162, 1981b
Termijtelen, A. and van Rood, J. J.: The role in primary MLC of the non HLA-D/DR determinant PL3A. Tissue Antigens 17: 57–63, 1981a
Termijtelen, A. and van Rood, J. J.: Complexity of stimulation in MLC and the influence of matching for HLA-A and -B. Scand. J. Immunol. 14: 459–466, 1981b
Termijtelen, A., Bradley, B. A., and van Rood, J. J.: A new determinant defined by PLT, coded for in the HLA region and apparently independent of the HLA-D and DR loci. Tissue Antigens 15: 267–274, 1980
Termijtelen, A., Shaw, S., and van Rood, J. J.: Identity of PL3A and SB1. Tissue Antigens 21: 400–401, 1983a
Termijtelen, A., van den Berge, S. J., and van Rood, J. J.: LB-Q1 and LB-Q2. Two new determinants defined in the primed lymphocyte test and independent of HLA-D/DR, MB/LB-E or SB. Hum. Immunol., in press, 1983b
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Termijtelen, A., Khan, P.M., Shaw, S. et al. Mapping SB in relation to HLA and GL01 using cells from first-cousin marriage offspring. Immunogenetics 18, 503–512 (1983). https://doi.org/10.1007/BF00364391
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00364391