Abstract
We have established unique restriction fragment length polymorphism (RFLP) patterns characteristic of homozygous typing cells (HTCs) for HLA-DR-1 through HLA-DR-8 haplotypes. These RFLP patterns were found to segregate in family members and correlate 100% with HLA-DR antibody phenotyping. The RFLP patterns were used to type chronic myelocytic leukemic cells which have a Philadelphia translocation from 23 randomly selected Caucasoid patients. The results show an alternative method for the determination of the HLA-DR types without using live cells and to study disease association with the HLA-DR region.
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Hui, K., Festenstein, H., de Klein, A. et al. HLA-DR genotyping by restriction fragment length polymorphism analyses. Immunogenetics 22, 231–239 (1985). https://doi.org/10.1007/BF00404482
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DOI: https://doi.org/10.1007/BF00404482