Abstract
T-lymphocyte clones specific for the influenza A/Texas virus were obtained by limiting dilution of activated T cells from an HLA A2/3, B7/39, Cw -/-, DR2-short/2 short, DQw1/w1, DwFJO/FJO donor. Among the proliferating clones studied, and irrespective of their antigenic specificities, most of them were restricted by epitope(s) on HLA-DR molecules present only on DR2-short/DwFJO cells but not on DR2-negative or DR2-long positive (Dw2, Dw12, Dw-) cells. Two clones were restricted by epitopes borne by DQ products. Here again, these epitopes were present on DR2-short/DwFJO but not on DR2-long, DQw1 (Dw2, Dw12) cells, indicating that the DQwl molecules of DR2-long and DR2-short haplotypes are different. Taken together, these results indicate that the DR2-short, DwFJO haplotype is characterized by both HLA-DR- and DQ-specific molecules. Finally, one clone was restricted by an epitope shared by DR products from DR2 short/DwFJO, DRw11, and DRw13 haplotypes. This latter functional determinant has never been described until now.
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Abbreviations
- APC:
-
antigen-presenting cells
- HAU:
-
hemagglutinin units of influenza virus
- HLA:
-
human leukocyte antigens
- HTC:
-
homozygous typing cells
- IL-2:
-
interleukin 2
- mAb:
-
monoclonal antibody
- MHC:
-
major histocompatibility complex
- MLR:
-
mixed lymphocyte reactions
- PBM:
-
peripheral blood mononuclear cells
- %RR:
-
relative response percent
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Zeliszewski, D., Sterkers, G., Freidel, C. et al. Functional definition of HLA-DR and -DQ epitopes specific for DR2-short, DwFJO haplotype. Immunogenetics 23, 385–392 (1986). https://doi.org/10.1007/BF00372671
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DOI: https://doi.org/10.1007/BF00372671