Abstract
Homozygous typing cells (HTC) were primed, using responding and stimulating lymphocytes of the same HLA-D groups. These intra-HLA-D group primings showed strong specific responses. Restimulation by HLA-D heterozygous and homozygous cell panels showed no correlation between the restimulating determinant and HLA-D. On the other hand, an unrelated individual, not carrying Dw4 and primed to Dw4 HTC, is restimulated by three of four Dw4-HTC. Thus, one non-HLA-D-associated restimulating determinant and another HLA-D-associated determinant could be identified. The differences among the four Dw4 HTC recognized in secondary MLC could reflect either recognition of separate gene products or recognition of separate determinants on the same gene product.
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Wank, R., Schendel, D.J., Hansen, J.A. et al. The lymphocyte restimulation system: Evaluation by intra-HLA-D group priming. Immunogenetics 6, 107–115 (1978). https://doi.org/10.1007/BF01563901
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DOI: https://doi.org/10.1007/BF01563901