Summary
During batch cultivation of Catharanthus roseus cell suspensions, alkaloids were found in the culture medium after growth had ceased. Resting cell suspensions with high alkaloid content were obtained by transferring the cells to a medium devoid of 2.4 D (2.4 dichlorophenoxy-acetic acid). A production system with continuous feeding was developped to study alkaloid production by these resting cell suspensions.
Similar content being viewed by others
References
Deus B, Zenk MH (1982) Exploitation of plant cells for the production of natural compounds. Biotechnol Bioeng 24:1965–1974
Döller G (1978) Influence of the medium on the production of serpentine by suspension cultures of Catharanthus roseus (L.) G. Don. In: Alfermann AW, Reinhard E (eds) Production of natural compounds by cell culture methods. Proceedings of Int Symp on Plant Cell Culture, München, pp 109–117
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Knoblock KH, Berlin J (1980) Influence of medium composition on the formation of secondary compounds in cell suspension cultures of Catharanthus roseus (L.) G. Don. Z Naturforsch [C] 35:551–556
Kurz WGW, Chatson KB, Constabel F, Kutney JP, Choi LSL, Kolodziejczyk P, Seigh SK, Stuart KL, Worth BR (1980) Alkaloid production in Catharanthus roseus cell cultures. I. Initial studies on cell lines and their alkaloid content. Phytochem 19:2538–2587
Pech JC, Romani RJ (1979) Senescence of pear fruit cells cultured in a continuous renewed, auxin-deprived medium. Plant Physiol 63:814–817
Pétiard V (1980) Mise en évidence d'alcaloídes dans le milieu nutritif de cultures de tissues de Catharanthus roseus G. Don. Physiol Vég 18:331–337
Roustan JP, Ambid C, Fallot J (1982) Influence de l'acide 2–4 dichlorophénoxyacétique sur l'accumulation de certains alcaloídes indoliques dans les cellules quiescentes de Catharanthus roseus cultivées in vitro. Physiol Vég 20:523–532
Vinas R, Pareilleux A (1982) Production d'alcaloídes par des suspensions cellulaires de Catharanthus roseus cultivées in vitro. Physiol Vég 20:219–225
Zenk MH, El-Shagi H, Arens H, Stöckigt J, Weiler EW, Deus B (1977) Formation of indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its biotechnological application. Springer, Berlin, pp 27–43
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pareilleux, A., Vinas, R. A study on the alkaloid production by resting cell suspensions of Catharanthus roseus in a continuous flow reactor. Appl Microbiol Biotechnol 19, 316–320 (1984). https://doi.org/10.1007/BF00253778
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00253778