Summary
About 300 strains of bifidobacteria were examined for their capacity to release L-isoleucine in the fermentation broth. A strain of the speciesBifidobacterium ruminale was selected as the best producer. After treatment of this strain with NTG a DL-α-aminobutyric acid-resistant mutant capable of producing about 5 mg/ml of L-isoleucine in presence of 1.5 % DL-α-aminobutyric acid was obtained. Cultural conditions and acetohydroxyacid synthetase activity were studied.
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Matteuzzi, D., Crociani, F., Emaldi, O. et al. Isoleucine production in bifidobacteria. European J. Appl Microbiol. 2, 185–194 (1976). https://doi.org/10.1007/BF00930879
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DOI: https://doi.org/10.1007/BF00930879