Skip to main content
SpringerLink
Log in

Extractive bioconversion in aqueous two-phase systems

Production of prednisolone from hydrocortisone using Arthrobacter simplex as catalyst

  • Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The transformation of hydrocortisone to prednisolone was studied in aqueous two-phase systems, as a model for the extractive bioconversion of fine chemicals. The bacterium, Arthrobacter simplex, was able to grow in the two-phase system and the cells could be revitalized after a period of use. Use of aqueous two-phase systems made it possible to operate the reaction at higher substrate concentrations than in pure buffer solution. An adsorptive method to remove the product from the top phase was tested and shown to be both efficient and compatible with the overall process. In order to reduce the costs of operation in aqueous two-phase systems, a cheaper starch-based polymer, Reppal-PES, was successfully used as a substitute for dextran.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adlercreutz P, Mattiasson B (1982) Oxygen supply by hemoglobin or emulsions of perfluorochemicals. Eur J Appl Microbiol Biotechnol 16:165–170

    Google Scholar 

  • Antonini E, Carrea G, Cremonesi P (1981) Enzyme catalyzed reactions in water-organic solvent two-phase systems. Enzyme Microb Technol 3:291–296

    Google Scholar 

  • Bhasin DP, Gryte CG, Studebaker JF (1976) A silicone polymer as a steroid reservoir for enzyme-catalyzed steroid reactions. Biotechnol Bioeng 18:1777–1792

    Google Scholar 

  • Brink LES, Tramper J (1985) Optimization of organic solvent in multiphase biocatalysis. Biotechnol Bioeng 27:1258–1269

    Google Scholar 

  • Buckland BC, Dunnill P, Lilly MD (1975) The enzymatic transformation of water-insoluble reactants in non-aqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a Nocardia sp. Biotechnol Bioeng 17:815–826

    Google Scholar 

  • Constantinides A (1980) Steroid transformation at high substrate concentrations using immobilized Corynebacterium simplex cells. Biotechnol Bioeng 22:119–136

    Google Scholar 

  • Kaul R, Adlercreutz P, Mattiasson B, Co-immobilization of substrate and biocatalyst. A method for bioconversion of poorly soluble substances in water milieu. Biotechnol Bioeng (in press)

  • Kondo E, Masuo E (1961) ‘Pseudo-crystallofermentation’ of steroid: A new process for preparing prednisolone by a microorganism. J Gen Appl Microbiol 7:113–117

    Google Scholar 

  • Leonhardt A, Szwajcer E, Mosbach K (1985) The potential use of silicon compounds as oxygen carriers for free and immobilized cells containing l-amino acid oxidase. Appl Microbiol Biotechnol 21:162–166

    Google Scholar 

  • Lilly MD, Woodley JM (1985) Biocatalytic reactions involving water-insoluble organic compounds. In: Tramper J, Van der Plas HC, Linko P (ed) Biocatalysts in organic syntheses. Elsevier, Amsterdam, p 179

    Google Scholar 

  • Mattiasson B (1983) Applications of aqueous two-phase systems in biotechnology. Trends Biotechnol 1:16–20

    Google Scholar 

  • Mattiasson B, Hahn-Hägerdal B (1983) Utilization of aqueous two-phase systems for generating soluble immobilized preparations of biocatalysts. In: Mattiasson B (ed) Immobilized cells and organelles. CRC-Press, Florida, Vol I, p 121

    Google Scholar 

  • Mattiasson B, Larsson M (1985) Extractive bioconversions with emphasis on solvent production. In: Russel GE (ed) Biotechnology and genetic engineering reviews. Intercept, England, Vol 3, p 137

    Google Scholar 

  • Mattiasson B, Ling TGI (1986) Efforts to integrate affinity interactions with conventional separation technologies. J Chromat 376:235–243

    Google Scholar 

  • Ohlson S, Larsson P-O, Mosbach K (1979) Steroid transformation by living cells immobilized in calcium alginate. Eur J Appl Microbiol Biotechnol 7:103–110

    Google Scholar 

  • Puziss M, Hedén C-G (1965) Toxin production by Clostridium tetani in biphasic liquid cultures. Biotechnol Bioeng 7:355–366

    Google Scholar 

  • Sonomoto K, Tanaka A, Omata T, Yamane T, Fukui S (1979) Application of photocrosslinkable resin prepolymers to entrap microbial cells. Effects of increased gel hydrophobicity on the hydrocortisone 265-1. Eur J Appl Microbiol Biotechnol 6:325–334

    Google Scholar 

  • Wang HY (1983) Integrating biochemical separation and purification steps in fermentation processes. Ann NY Acad Sci 413:313–321

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Chemical Center, University of Lund, P.O.B. 124, S-221 00, Lund, Sweden

    Rajni Kaul & Bo Mattiasson

Authors
  1. Rajni Kaul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bo Mattiasson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Professor Dr. Georg Manecke on the occasion of his 70th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaul, R., Mattiasson, B. Extractive bioconversion in aqueous two-phase systems. Appl Microbiol Biotechnol 24, 259–265 (1986). https://doi.org/10.1007/BF00257046

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00257046

Keywords

Search

Navigation