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Influence of oxygen and substrate supply on the metabolism of Candida maltosa during cultivation on n-alkanes

  • Applied Microbiology
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Summary

The influence of different states of oxygen and alkane substrate supply on the metabolism of Candida maltosa during cultivation on n-alkanes has been investigated. At sufficient oxygen and substrate supply a nearly equimolar ratio between the formation of biomass and alkane oxidation was observed. About 45% of the carbon source utilized was incorporated into the biomass. Strong oxygen limitation decreased protein formation and carbon incorporation into the biomass with a simultaneous increase in CO2 formation, whereas periodic changes of oxygen supply only caused a decrease in carbon incorporation into the biomass and an increase in CO2 formation. During cultivation in the presence of an inert hydrocarbon (pristane) it was found that carbon limitation and oxygen saturation diminished the formation of total and nitrogen-containing biomass, whereas carbon and oxygen limitation reduced the formation of total biomass.

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References

  • Bright R, Dyer P (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    CAS  Google Scholar 

  • Davidov YR, Gololobov AD (1980) Effect of pO2 on the regulation of metabolism in yeast during cultivation on n-alkanes. Proceedings of the International US/USSR Conference of Mechanism Kinetic of Growth on Various Substrates; Control, Simulation and Optimisation of Microbiological Processes, Proj. I–II, PB81-219131, pp 94–103

  • Dole VP (1956) A relation between non-esterified fatty acids in plasma and metabolism of glucose. J Clin Invest 35:150–156

    Google Scholar 

  • Einsele A, Fiechter A (1971) Liquid and solid hydrocarbons. Adv Biochem Eng 1:169–194

    Google Scholar 

  • Gholson R, Baptist JK, Coon MJ (1963) Hydrocarbon oxidation by a bacterial enzyme system. II. Cofactor requirements for octanol formation from octane. Biochemistry 2:1155–1159

    Google Scholar 

  • Glombitza F, Heinritz B (1979) Thermodynamik mikrobieller Prozesse. Z Allg Mikrobiol 19:171–176

    Google Scholar 

  • Gmünder FK, Käppeli O, Fiechter A (1981) Chemostat studies on the hexadecane assimilation by the yeast Candida tropicalis. II. Regulation of cytochromes and enzymes. Eur J Appl Microbiol Biotechnol 12:135–142

    Google Scholar 

  • Heinritz B, Bley T, Ringpfeil M (1985) Einsatz von Hochleistungsreaktoren zur mikrobiologischen Stoffwandlung. Chem Technol 37:514–516

    Google Scholar 

  • Honeck H, Schunck W-H, Riege P, Müller H-G (1982) The alkane monooxygenase system of the yeast Lodderomyces elongisporus: purification and some properties of NADPH-cytochrome P-450 reductase. Biochem Biophys Res Commun 106:1318–1324

    Google Scholar 

  • Huth J (1988) Über die Verwentung von n-Alkanen durch Candida maltosa EH 15D unter besonderer Berücksichtigung der festen n-Alkane mit 20 und mehr C-Atomen. Dissert AdW der DDR, Berlin

    Google Scholar 

  • Huth J, Müller H-G, Honeck H, Schunck W-H, Röstel S (1983) Verfahren zur Bestimmung der Biomasseproduktion auf stickstoffreeien Kohlenstoffquellen. DD-WP 218 688, 601 N 33/84

  • Huth J, Röstel S, Schunck W-H (1984) Verfahren zur Lagerung enzymatisch aktiver n-Alkan verwertender Hefekulturen. DD-WP 231 574 C 12N 1/28

  • Katinger HWD (1976) Physiological response of Candida Tropicalis grown on n-paraffin to mixing in a tubular closed loop fermenter. Eur J Appl Microbiol Biotechnol 3:103–114

    Google Scholar 

  • Mauersberger S, Matyashova RN, Müller H-G, Lozinov AB (1980) Influence of the growth substrate and the oxygen concentration in the medium on the cytochrome P-450 content in Candida guilliermondii. Eur J Appl Microbiol Biotechnol 9:285–294

    Google Scholar 

  • Mauersberger S, Schunck W-H, Müller H-G (1981) The induction of cytochrome P-450 in Lodderomyces elongisporus. Z Allg Mikrobiol 21:313–321

    Google Scholar 

  • Mauersberger S, Schunck W-H, Müller H-G (1984) The induction of cytochrome P450 in the alkane-utilizing yeast Lodderomyces elongisporus: alterations in the microsomal membrane fraction. Eur J Appl Microbiol Biotechnol 19:29–35

    Google Scholar 

  • Müller H-G, Schunck W-H, Riege P, Honeck H (1984) Cytochrome P-450 of microorganisms. In: Cytochrome P-450. Ruckpaul K, Rein H (eds) Akademie-Verlag, Berlin, pp 337–369

    Google Scholar 

  • Riege P, Schunck W-H, Honeck H, Müller H-G (1981) Cytochrome P-450 from Lodderomyces elongisporus: its purification and some properties of the highly purified protein. Biochem Biophys Res Commun 98:527–534

    Google Scholar 

  • Sanglard P, Käppeli O, Fiechter A (1984) Metabolic conditions determining the composition and catalytic activity of cytochrome P-450 monooxygenase in Candida tropicalis. J Bacteriol 157:297–301

    Google Scholar 

  • Schunck W-H, Mauersberger S, Kärgel E, Huth J, Müller H-G (1987a) Function and regulation of cytochrome P-450 in alkane assimilating yeast. II. Effect of oxygen-limitation. Arch Microbiol 147:245–248

    Google Scholar 

  • Schunck W-H, Mauersberger S, Huth J, Riege P, Müller H-G (1987b) Function and regulation of cytochrome P-450 in alkane assimilating yeast. I. Selective inhibition with carbon monoxide in growing cells. Arch Microbiol 147:240–244

    Google Scholar 

  • Sokolov DP, Lirova SA, Senjuschkin AA, Rabotnova IL (1984) Effect of cyclic changes in pH and pO2 on a chemostat Candida utilis culture. Mikrobiologija (Belgr) 53:772–776

    Google Scholar 

  • Vercooyen AHM, Rietema K (1980) Growth of yeast on n-alkanes. III. Batch experiments. Biotechnol Bioeng 22:615–637

    Google Scholar 

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Riege, P., Blasig, R., Müller, HG. et al. Influence of oxygen and substrate supply on the metabolism of Candida maltosa during cultivation on n-alkanes. Appl Microbiol Biotechnol 32, 101–107 (1989). https://doi.org/10.1007/BF00164830

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  • DOI: https://doi.org/10.1007/BF00164830

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