Summary
The effect of inorganic phosphate concentrations on antibiotic and extracellular protein production by Myxococcus coralloides D have been examined. Antibiotic production by growing cells of this myxobacterium was maximal at phosphate concentrations of 10–20 mM, but was inhibited by concentrations higher than 20 mM. The total extracellular protein and the extracellular protein per cell ratio were independent of phosphate levels in the culture broth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arias JM, Montoya E (1978) Dispersed growth and cell lysis in Myxococcus coralloides. Microbios Lett 5:81–84
Arias JM, Rodriguez C, Montoya E (1979) Purification and partial characterization of an antibiotic produced by Myxococcus coralloides. J Antibiot 32:205–211
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Fernandez-Vivas A, Arias JM, Montoya E (1983) Autolysis in Myxococcus coralloides D. FEMS Microbiol Lett 20:97–101
Gonzalez F, Arias JM, Montoya E (1987) Phosphatase activities in the life cycle of Myxococcus coralloides D. J Gen Microbiol 133:2327–2332
Martin JF (1977) Control of antibiotic synthesis by phosphate. Adv Biochem Eng 6:105–127
Martin JF, Demain AL (1980) Control of antibiotic biosynthesis. Microbiol Rev 44:230–251
Rosenberg E, Varon M (1984) Antibiotics and lytic enzymes. In: Rosenberg E (ed) Myxobacteria: development and cell interactions. Springer, Berlin, Heidelberg, New York, pp 109–125
Shimkets LJ (1984) Nutrition, metabolism and the initiation of development. In: Rosenberg E (ed) Myxobacteria: development and cell interactions. Springer, Berlin, Heidelberg, New York, pp 91–108
Tsukagoshi N, Yamada H, Tsuboi A, Udaka S (1981) Effects of phosphate in medium on protein secretion in a protein-producing bacterium, Bacillus brevis 47. Appl Environ Microbiol 42:370–374
Author information
Authors and Affiliations
Additional information
Offprint requests to: J. M. Arias
Rights and permissions
About this article
Cite this article
Gonzalez, F., Montoya, M.D., Fárez, E. et al. Effect of phosphate on antibiotic and extracellular protein production by Myxococcus coralloides D. Appl Microbiol Biotechnol 33, 78–80 (1990). https://doi.org/10.1007/BF00170575
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00170575