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High-level expression of the ice-nucleating activity of Pseudomonas syringae in relation to its growth characteristics

  • Applied Genetics and Regulation
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Abstract

Optimization of the culture medium is essential for the production of a large biomass of high ice-nucleating-active micro-organisms such as Pseudomonas syringae. Cultures in bioreactors show that optimal substrate concentrations are approximately the same for ice nucleating activity (INA) and total biomass (50–80 g/l of glucose; 28 g/l of peptone) but not for the growth rate. The INA is lowest when the growth rate is highest (50 g/l of glucose, 15 g/l of peptone). We have shown that the maximal biomass production and INA are related to the C/N ratio (optimal ratio: 10) rather than to the substrate concentration. These results also contribute to knowledge on the physiology of these bacteria and support the sequential maturation of the ice nucleating sites.

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  1. Départment de Génie Chimique, Division des Procédés Biotechnologiques, Université de Technologie de Compiègne, BP 649, 60206, Compiégne Cedex, France

    A. Blondeaux & N. Cochet

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  1. A. Blondeaux
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  2. N. Cochet
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Blondeaux, A., Cochet, N. High-level expression of the ice-nucleating activity of Pseudomonas syringae in relation to its growth characteristics. Appl Microbiol Biotechnol 42, 116–120 (1994). https://doi.org/10.1007/BF00170233

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  • DOI: https://doi.org/10.1007/BF00170233

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