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Biosynthesis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1,3-butanediol or from 3-hydroxybutyrate by Pseudomonas sp. A33

  • Applied Microbial and Cell Physiology
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Abstract

Pseudomonas sp. A33 and other isolates of aerobic bacteria accumulated a complex copolyester containing 3-hydroxybutyric acid (3HB) and various medium-chain-length 3-hydroxyalkanoic acids (3HAMCL) from 3-hydroxybutyric acid or from 1,3-butanediol under nitrogen-limitated culture conditions. 3HB contributed to 15.1 mol/100 mol of the constituents of the polyester depending on the strain and on the cultivation conditions. The accumulated polymer was a copolyester of 3HB and 3HAMCL rather than a blend of poly(3HB) and poly(3HAMCL) on the basis of multiple evidence. 3-Hydroxyhexadecenoic acid and 3-hydroxyhexadecanoic acid were detected as constituents of polyhydroxyalkanoates, which have hitherto not been described, by13C nuclear magnetic resonance or by gas chromatography/mass spectrometric analysis. In total, ten different constituents were detected in the polymer synthesized from 1,3-butanediol by Pseudomonas sp. A33:besides seven saturated (3HB, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydrohexadecanoate) three unsaturated (3-hydroxydodecenoate, 3-hydroxytetradecenoate and 3-hydrohexadecanoate) hydroxyalkanoic acid constituents occured. The polyhydroxyalkanoate synthase of Pseudomonas sp. A33 was cloned, and its substrate specificity was evaluated by heterologous expression in various strains of P. putida, P. oleovorans and Alcaligenes eutrophus.

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Authors and Affiliations

  1. Department of Chemical Technology, College of Engineering, Seoul National University, 151-742, Seoul, Korea

    E. Y. Lee & C. Y. Choi

  2. Institut für Mikrobiologie der Georg-August-Universität Göttingen, Grisebachstrasse 8, D-37077, Göttingen, Germany

    D. Jendrossek & A. Schirmer

  3. Institut für Mikrobiologie der Westfälischen Wilhelms-Universität, Correnstrasse 3, D-48149, Münster, Germany

    A. Steinbüchel

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  1. E. Y. Lee
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  2. D. Jendrossek
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  3. A. Schirmer
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  4. C. Y. Choi
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  5. A. Steinbüchel
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Lee, E.Y., Jendrossek, D., Schirmer, A. et al. Biosynthesis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1,3-butanediol or from 3-hydroxybutyrate by Pseudomonas sp. A33. Appl Microbiol Biotechnol 42, 901–909 (1995). https://doi.org/10.1007/BF00191189

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  • DOI: https://doi.org/10.1007/BF00191189

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