Abstract
When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe−, Trp−, Tyr−) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid. The recombinant strain can be used to produce in high yield specifically ring-labelled 4-hydroxybenzoic acid from isotopically labelled glucose.
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Müller, R., Wagener, A., Schmidt, K. et al. Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid. Appl Microbiol Biotechnol 43, 985–988 (1995). https://doi.org/10.1007/BF00166913
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DOI: https://doi.org/10.1007/BF00166913