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Bioremediation of 2,4,6-trinitrotoluene-contaminated soils by two different aerated compost systems

  • Environmental Biotechnology
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Abstract

Two composting systems were compared on a laboratory scale as a bioredediation technology for degradation or immobilization of 2,4,6-trinitrotoluene (TNT) in contaminated soils. The first compost was aerated from the beginning whereas the second compost was only aerated after an anaerobic prephase of 65 days. In the first compost system the TNT concentration declined rapidly by 92% but, at the end, TNT could be partially recovered. During the anaerobic prephase of the second compost system, TNT was almost completely converted to aminodinitrotoluenes, which during the subsequent aeration almost entirely disappeared. In addition, the second compost generated less toxic material than the first one as confirmed by inhibition of bioluminescence ofVibrio fischeri. These data show that microbiological TNT-degradation systems can be successfully designed which are prerequisite for an efficient bioremediation of contaminated soils.

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References

  • Beuth Verlag (1991) Normenausschuß Wasserwesen (NAW) im DIN Deutsches Institut für Normung e.V.

  • Bollag J-M, Myers C (1992) Detoxification of aquatic and terrestical sites through binding of pollutants to humic substances. Sci Total Environ 117/118:357–366

    Google Scholar 

  • Boopathy R, Kulpa CF, Wilson M (1993) Metabolism of 2,4,6-trinitrotoluene (TNT) byDesulfovibrio sp. (B strain). Appl Microbiol Biotechnol 39:270–275

    Google Scholar 

  • Boopathy R, Manning J, Montemagno C, Kulpa C (1994) Metabolism of 2,4,6-trinitrotoluene by aPseudomonas consortium under aerobic conditions. Curr Microbiol 28:131–137

    Google Scholar 

  • Breitung J, Bruns-Nagel D, Löw Ev, Steinbach K, Kaminski L, Haas R, Gemsa D (1995) Mikrobielle Sanierung von 2,4,6-Trinitrotoluol (TNT) kontaminierten Böden. UWSF-Z. Umweltchem. Ökotox. 7:195–200

    Google Scholar 

  • Fernando T, Bumpus JA, Aust SD (1990) Biodegradation of TNT (2,4,6-trinitrotoluene) byPhanarochaete chrysosporium. Appl Environ Microbiol 56:1666–1671

    Google Scholar 

  • Helling CS, Krivonak AE (1978) Physiochemical characteristics of bound dinitroaniline herbicides in soil. J Agric Food Chem 26:1156–1163

    Google Scholar 

  • Hsu TS, Bartha R (1974) Biodegradation of chloroaniline humus complexes in soil and in culture solution. Soil Sci 118:213–219

    Google Scholar 

  • Isbister JD, Anspach GL, Kitchens JF, Doyle RC (1984) Composting for decontamination of soils containing explosives. Microbiologica 7:47–73

    Google Scholar 

  • Kaplan DL, Kaplan AM (1982) Thermophilic biotransformations of 2,4,6-trinitrotoluene under simulated composting conditions. Appl Environ Microbiol 44:757–760

    Google Scholar 

  • Klibanov AM, Alberti BN, Morris J, Felshin LM (1980) Enzymatic removal of toxic phenols and anilins from waste waters. J Appl Biochem 2:414–421

    Google Scholar 

  • Michels J, Gottschalk G (1994) Inhibition of the lignin peroxidase ofPhanarochaete chrysosporium by hydroxylamino-dinitrotoluene, and early intermediate in the degradation of 2,4,6-trinitrotoluene. Appl Environ Microbiol 60:187–194

    Google Scholar 

  • Osmon JL, Andrews CC (1978) The biodegradation of TNT in enhanced soil and compost systems. (U.S. Army Armament Research and Development Command, ARLCD-TR-77032, Dover, N.J. Technical Information Service Publication no. ADE 400073) National Technical Information Service, Springfield. Va

    Google Scholar 

  • Parris GE (1980) Covalent binding of aromatic amines to humanes. 1. Reactions with carbonyls and quinons. Environ Sci Technol 14:1099–1106

    Google Scholar 

  • Preuss A, Fimpel J, Diekert G (1993) Anaerobic transformation of 2,4,6-trinitrotoluene (TNT). Arch Microbiol 159:345–353

    Google Scholar 

  • Shindo H, Huang PM (1984) Catalytic effects of manganese (IV), iron (III), aluminum and silicon oxide on the formation of phenolic polymers. Soil Sci Soc Am J 48:927–934

    Google Scholar 

  • Vorbeck C, Lenke H, Fischer P, Knackmus H-J (1994) Identification of a Hydride-Meisenheimer complex as a metabolite of 2,4,6-trinotrotoluene by aMycobacterium strain. J Bacteriol 176:932–934

    Google Scholar 

  • Williams RT, Ziegenfuss PS, Sisk WE (1992) Composting of explosives and propellant contaminated soils under thermophilic and mesphilic conditions. J Ind Microbiol 9:137–144

    Google Scholar 

  • Ziechmann W (1977) Zwischenmolekulare Kräfte und die Struktur von Huminstoffen. Z Pflanzenernähr Bodenkd 140:151–157

    Google Scholar 

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Breitung, J., Bruns-Nagel, D., Steinbach, K. et al. Bioremediation of 2,4,6-trinitrotoluene-contaminated soils by two different aerated compost systems. Appl Microbiol Biotechnol 44, 795–800 (1996). https://doi.org/10.1007/BF00178621

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  • DOI: https://doi.org/10.1007/BF00178621

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