Abstract
Pseudomonas fluorescens NCIMB 11671 metabolises α-pinene via α-pinene oxide and 2-methyl-5-isopropylhexa-2,5-dienal. Mutants unable to grow on α-pinene and/or α-pinene oxide have been isolated by N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis, including an unexpected phenotype able to grow on α-pinene but not on α-pinene oxide. The mutants have been classified on the basis of their α-pinene monooxygenase, α-pinene oxide lyase and aldehyde dehydrogenase activities. Biotransformation of α-pinene by the wild-type and mutant strains has revealed evidence for alternative routes for pinene metabolism to that already proposed.
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Received: 24 August 1995/Received revision: 20 December 1995/Accepted: 8 January 1996
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Colocousi, A., Saqib, K. & Leak, D. Mutants of Pseudomonas fluorescens NCIMB 11671 defective in the catabolism of α-pinene. Appl Microbiol Biotechnol 45, 822–830 (1996). https://doi.org/10.1007/s002530050769
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DOI: https://doi.org/10.1007/s002530050769