Abstract
In order to test the possibility of utilizing high pressure in bioscience and biotechnology, a simple method for high-pressure generation and its use for microbial inactivation have been studied. When a pressure vessel was filled with water, sealed tightly and cooled to sub-zero temperatures, high pressure was generated in the vessel. The pressure generation was 60 MPa at −5 °C, 103 MPa at −10 °C, and 140 MPa at −15 °C, −20 °C, and −22 °C. The high pressure generated inactivated microorganisms effectively: yeasts (Saccharomyces cerevisiae and Zygosaccharomyces rouxii), bacteria (Lactobacillus brevis and Eschericia coli), and fungi (Aspergillus niger and Aspergillus oryzae) were completely inactivated when stored in sealed vessels −20 °C for 24 h. However, Staphylococcus aureus was only partly inactivated under the same conditions. This method opens up a new application of high pressure for storing, transporting, and sterilizing of foods and biological materials.
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Received: 28 July 1997 / Received last revision: 12 June 1998 / Accepted: 19 June 1998
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Hayakawa, K., Ueno, Y., Kawamura, S. et al. Microorganism inactivation using high-pressure generation in sealed vessels under sub-zero temperature. Appl Microbiol Biotechnol 50, 415–418 (1998). https://doi.org/10.1007/s002530051313
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DOI: https://doi.org/10.1007/s002530051313