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Interleukin-2-dependent long-term cultures of low-density lymphocytes allow the proliferation of lymphokine-activated killer cells with natural killer, Tiγ/δ or TNK phenotype

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Summary

We have developed a culture system for “longterm” growth of human lymphokine-activated killer (LAK) cells exhibiting an elevated, wide-spectrum antitumor cytotoxicity. The system allows the exponential growth of monocyte-depleted low-density lymphocytes in the presence of human serum and recombinant human interleukin-2 (103 U/ml), alone or in combination with interleukin-1 α or β (both at 10 U/ml). Eighteen cultures were established from 18 normal adult donors. The membrane phenotypes of the final LAK cell population, assessed by a panel of monoclonal antibodies (mAb), consist of three main types: (a) NKH-1+, Tiα/β−, Tiγ/δ−, and CD3− lymphocytes; (b) NKH-1+, Tiα/β−, Tiγ/δ+, and CD3+ lymphocytes and (c) NKH-1+, Tiα/β+, Tiγ/δ− and CD3+ lymphocytes. Northern blot analysis showed that all these cell populations express relatively high levels of perforin RNA, particularly cells exhibiting the first phenotype. This culture system may provide a tool for cellular and molecular studies on the mechanisms of antitumor cytotoxicity, as well as the basis for new adoptive immunotherapy protocols in advanced cancer.

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Authors and Affiliations

  1. Department of Hematology-Oncology, Istituto Superiore di Sanità, Viale Regina Elena, 299, I-00161, Rome, Italy

    U. Testa, A. Care, E. Montesoro, C. Fossati, G. Giannella & R. Masciulli

  2. Clinica Medica I, Policlinico Monteluce, Perugia, Italy

    M. Fagioli, D. Bulgarini, D. Habetswallner, P. G. Pelicci & C. Peschle

  3. Blood Transfusion Center, University “La Sapienza”, Rome, Italy

    G. Isacchi

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  1. U. Testa
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Testa, U., Care, A., Montesoro, E. et al. Interleukin-2-dependent long-term cultures of low-density lymphocytes allow the proliferation of lymphokine-activated killer cells with natural killer, Tiγ/δ or TNK phenotype. Cancer Immunol Immunother 31, 11–18 (1990). https://doi.org/10.1007/BF01742490

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