Summary
Soluble proteins of a granule fraction derived from human neutrophils were subjected to electrophoresis on 15% acrylamide gels at pH 4.3 Several of the protein bands were shown to possess hydrolytic activity on naphthol AS-D chloroacetate at pH 7.4. Electrophoretic movement of NASDCA esterases is similar to those of trypsin, chymotrypsin and elastase.
Zusammenfassung
Lösliche Proteine einer Granulafraktion aus menschlichen Neutrophilen wurden einer Acrylamidgel-Elektrophorese (15% Acrylamidgel bei pH 4,3) unterwerfen. Verschiedene Proteinbanden zeigten eine hydrolytische Aktivität gegenü ber Naphthol-AS-D-Chloracetat bei pH 7,4. Die elektrophoretische Wanderung der Naphthol-AS-D-Chloracetat spaltenden Esterase ist ähnlich der von Trypsin, Chymotrypsin und Elastase.
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Rindler, R., Schmalzl, F., Hörtnagl, H. et al. Naphthol AS-D chloroacetate esterases in granule extracts from human neutrophil leukocytes. Blut 23, 223–227 (1971). https://doi.org/10.1007/BF01633774
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DOI: https://doi.org/10.1007/BF01633774