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Surface glycoproteins (S-GP) on normal and malignant human leukocytes

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Summary

This study aimed to investigate high molecular weight surface glycoprotein (S-GP) patterns on various types of human leukocytes. S-GP were externally labelled by the Galactose-oxidase-NaB3H4 technique. Results based on the analysis of 120 samples derived from different types of normal and malignant leukocytes indicate that (i) the relative expression of high molecular weight S-GPs changes during haemopoietic cell differentiation and (ii) to some extent these changes enable the classification of human leukocytes.

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References

  1. Andersson LC, Gahmberg CG, Nilsson K, Wigzell H (1977) Surface glycoprotein patterns of normal and malignant human lymphoid cells. I. T cells, T blasts and leukemic T cell lines. Int J Cancer 20: 702–707

    Google Scholar 

  2. Andersson LC, Gahmberg CG (1978) Surface glycoproteins of human white blood cells. Analysis by surface labeling. Blood 52: 57–67

    Google Scholar 

  3. Andersson LC, Gahmberg CG, Simes MA, Teerenhovi L, Vuopio P (1979) Cell surface glycoprotein analysis: A diagnostic tool in human leukemias. Int J Cancer 23: 306–311

    Google Scholar 

  4. Bennet JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (1976) Proposals for the classification of the acute leukemias. Br J Haematol 33: 451–458

    Google Scholar 

  5. Bonner WM, Laskey RA (1974) A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46: 83–88

    Google Scholar 

  6. Bøyum A (1969) Separation of leukocytes from blood and bone marrow. Scand J Clin Lab Invest [Suppl] 97

  7. Gahmberg CG, Hakomori S (1976) Organization of glycoprotein and glycolipid in the plasma membrane of normal and transformed cells as revealed by galactose oxidase. In: Manson (Ed) Biomembranes. Wistar Press, Philadelphia, 8: 131–165

    Google Scholar 

  8. Gahmberg CG, Andersson LC (1978) Identification and characterization of normal and malignant human blood leukocytes by surface glycoprotein patterns. Ann NY Acad Sci 312: 240–255

    Google Scholar 

  9. Gattringer C, Huber H (1983) Immunological identification and in vitro activity of suppressor cells. Wien Klin Wochenschr 5: 138–144

    Google Scholar 

  10. Huber Ch, Rodt H, Thierfelder S, Braunsteiner H (1979) Surface properties and in vitro functional capacities of human T lymphocyte subsets separated by linear density gradients. J Immunol 123: 405–411

    Google Scholar 

  11. Huber Ch, Fink U, Leibold W, Schmalzl F, Peterson PA, Klareskog L, Braunsteiner H (1981) The role of adherent HLA-DR+ mononuclear cells in autologous and allogeneic MLR. J Immunol 127: 726–731

    Google Scholar 

  12. Huber Ch, Merkenschlager M, Gattringer C, Royston I, Fink U, Braunsteiner H (1982) Human autologous mixed lymphocyte reactivity is primarily specific for xenoprotein determinants adsorbed to antigen-presenting cells during rosette formation. J Exp Med 155: 1222–1227

    Google Scholar 

  13. Huber Ch, Lutz D, Niederwieser D, Weiser G, Dietze O, Theml H, Braunsteiner H (1982) Prognostic significance of lymphocyte density distribution profiles in adult non-Hodgkin lymphomas. Blood 60: 1397–1401

    Google Scholar 

  14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685

    Google Scholar 

  15. Nilsson K, Andersson LC, Gahmberg CG, Wigzell H (1977) Surface glycoprotein patterns of normal and malignant human lymphoid cells. II. B cells, B blasts and Epstein-Barr Virus (EBV)-positive and -negative B lymphoid cell lines. Int J Cancer 20: 708–716

    Google Scholar 

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Leiter, E., Pfister, R., Lutz, D. et al. Surface glycoproteins (S-GP) on normal and malignant human leukocytes. Blut 50, 157–168 (1985). https://doi.org/10.1007/BF00320072

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  • DOI: https://doi.org/10.1007/BF00320072

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