Summary
Leukemic cells of a 20 year old patient, suffering from acute lymphoblastic leukemia, were characterized by surface marker and functional analysis. A significant cell population within this type of leukemia expresses concomitantly the CD4 and CD8 antigen on the same cell and might represent a new differentiation stage of T-cells with the γ/δ receptor. The leukemic cells show a distinct pattern of growth response to mitogens and lymphokines, which might correlate to their differentiation stage. Moreover, a “natural killer”-like activity can be induced in these cells by IL-2.
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Abbreviations
- FITC:
-
fluorescein isothiocyanate
- PE:
-
phycoerythrin
- IL-2:
-
interleukin 2;
- γ/δ TCR:
-
gamma/delta T cell receptor
- NK:
-
natural killer
- PBL:
-
peripheral blood lymphocytes
- T-ALL:
-
acute T lymphoblastic leukemia
- ConA:
-
concanavalin A
- PMA:
-
phorbol myristate acetate
- BM:
-
bone marrow
- IL-2R:
-
IL-2 receptor
- TdT:
-
terminal deoxynucleotidyl transferase
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Supported by the Deutsche Forschungsgemeinschaft (DFG Wi-728/3-1)
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Wilhelm, M., Tony, H.P. Characterization of an acute T lymphoblastic leukemia expressing the γ/δ T-cell receptor. Blut 61, 213–218 (1990). https://doi.org/10.1007/BF01744133
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DOI: https://doi.org/10.1007/BF01744133