Skip to main content
SpringerLink
Log in

Molecular characterization of CD34+ human hematopoietic progenitor cells

  • Review Article
  • Published:
Annals of Hematology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 133:157–165

    PubMed  Google Scholar 

  2. Andrews RG, Singer JW, Bernstein ID (1986) Monoclonal antibody 12-8 recognizes a 115 kD molecule present on both unipotent and multipotent hematopoietic colony forming cells and their precursors. Blood 67:842–845

    PubMed  Google Scholar 

  3. Titley I, Colman SM, Bühring HJ, Campos L, Castoldi GL, Garrido F, Gaudernack G, Girard JP, Inglés-Esteve J, Invernizzi R (1994) Report on the CD34 Cluster Workshop. In: Schlossman S et al. (eds) Leukocyte typing. V: White cell differentiation antigens. Oxford University Press, Oxford

    Google Scholar 

  4. Lansdorp PM, Dougherty GJ, Humphries RK (1989) CD34 epitopes. In: Knapp W et al. (eds) Leucocyte typing. IV: White cell differentiation antigens. Oxford University Press, Oxford, pp 826–827

    Google Scholar 

  5. Bender JG, Unverzagt KL, Walker DE, Lee W, Epps DEV, Smith DH, Stewart CC, To LB (1991) Identification and comparison of CD34- positive cells and their subpopulations from normal peripheral blood and bone marrow using flow cytometry. Blood 77:2591–2596

    PubMed  Google Scholar 

  6. Civin CI, Banquerigo ML, Strauss LC, Loken MR (1987) Antigenic analysis of hematopoiesis. IV. Flow cytometric characterization of My-10-positive progenitor cells in normal human bone marrow. Exp Hematol 15:10–17

    PubMed  Google Scholar 

  7. Egeland T, Steen R, Quarsten H, Gaudernack G, Yang YC, Thorsby E (1991) Myeloid differentiation of purified CD34 + cells after stimulation with recombinant human granulocyte-monocyte colony-stimulating factor (CSF), granulocyte-CSF, monocyte-CSF, and interleukin-3. Blood 78:3192–3199

    PubMed  Google Scholar 

  8. Ema H, Suda T, Miura Y, Nakauchi H (1990) Colony formation of clonesorted human hematopoietic progenitors. Blood 75:1914–1946

    Google Scholar 

  9. Sutherland HJ, Eaves CJ, Lansdorp PM, Phillips GL, Hogge DE (1994) Kinetics of committed and primitive blood progenitor mobilization after chemotherapy and growth factor treatment and their use in autotransplants. Blood 83: 3808–3814

    PubMed  Google Scholar 

  10. Traycoff CM, Abboud MR, Layer J, Brandt JE, Hoffman R, Law P, Ishizawa L, Srour EF (1994) Evaluation of the in vitro behavior of phenotypically defined populations of umbilical cord blood hematopoietic progenitor cells. Exp Hematol 22:215–222

    PubMed  Google Scholar 

  11. Mayani H, Dragowska W, Lansdorp PM (1993) Cytokine-induced selective expansion and maturation of erythroid versus myeloid progenitors from purified cord blood precursor cells. Blood 81:3252–3258

    PubMed  Google Scholar 

  12. Serke S, Sauberlich S, Huhn D (1991) Multiparameter flow-cytometrical quantitation of circulating CD34 + cells-correlation to the quantitation of circulating haemopoietic progenitor cells by in vitro colony assay. Br J Haematol 77:453–459

    PubMed  Google Scholar 

  13. Berenson RJ, Bensinger WI, Hill RS, Andrews RG, Garcialopez J, Kalamasz DF, Still BJ, Spitzer G, Buckner CD, Bernstein ID (1991) Engraftment after infusion of CD34 + marrow cells in patients with breast cancer or neuroblastoma. Blood 77:1717–1722

    PubMed  Google Scholar 

  14. Berenson RJ, Andrews RG, Bensinger WI, Kalamasz D, Knitter G, Buckner CD, Bernstein ID (1988) Antigen CD34 + marrow cells engraft lethally irradiated baboons. J Clin Invest 81:951

    PubMed  Google Scholar 

  15. Baum CM, Weissman IL, Tsukamoto AS, Buckle A-M, Peault B (1992) Isolation of a candidate human hematopoietic stem-cell population. Proc Natl Acad Sci USA 89:2804–2808

    PubMed  Google Scholar 

  16. Huang S, Terstappen LWMM (1992) Formation of haematopoietic microenvironment and heamatopoietic stem cells from single human bone marrow stem cells. Nature 360:745–749

    PubMed  Google Scholar 

  17. Simmons PJ, Torok-Storb B (1991) CD34 expression by stromal precursors in normal human adult bone marrow. Blood 78:2848–2853

    PubMed  Google Scholar 

  18. Beschorner WE, Civin CI, Strauss LC (1985) Localization of hematopoietic progenitor cells in tissue with the anti-My-10 monoclonal antibody. Am J Pathol 119:1

    PubMed  Google Scholar 

  19. Fina L, Molgaard HV, Robertson D, Bradley NJ, Monoghan P, Delia D, Sutherland DR, Baker MA, Greaves MF (1990) Expression of the CD34 gene in vascular endothelial cells. Blood 75:2417–2426

    PubMed  Google Scholar 

  20. Delia D, Lampugnani MG, Resnati M, Dejana E, Aiello A, Fontanella E, Soligo D, Pierotti MA, Greaves MF (1993) CD34 expression is regulated reciprocally with adhesion molecules in vascular endothelial cells in vitro. Blood 81:1001

    PubMed  Google Scholar 

  21. Nakamura Y, Komano H, Nakauchi H (1993) Two alternative forms of cDNA encoding CD34. Exp Hematol 21:236

    PubMed  Google Scholar 

  22. Simmons DL, Satterthwaite AB, Tenen DG, Seed B (1992) Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells. J Immunol 148:267–271

    PubMed  Google Scholar 

  23. Simmons PJ, Masinovsky B, Longenecker BM, Berenson R, Torok-Storb B, Gallatin WM (1992) Vascular cell adhesion molecule-1 expression by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells. Blood 80: 388–395

    PubMed  Google Scholar 

  24. Cyster JC, Somoza N, Killeen N, Williams AF (1990) Protein sequence and gene structure for mouse leukosialin (CD43), a T lymphocyte mucin without introns in the coding sequence. Eur J Immunol 20:875

    PubMed  Google Scholar 

  25. Fukuda M, Viitela J, Matteson J, Carlsson SR (1988) Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. J Biol Chem 263:18920

    PubMed  Google Scholar 

  26. Holnes CL, Simmons DL (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81:1607–1613

    PubMed  Google Scholar 

  27. Lasky LA, Singer MS, Dowbenko D, Imai Y, Henzel WJ, Grimley C, Fennie C, Gillett N, Watson SR, Rosen SD (1992) An endothelial ligand for L-selectin is a novel mucin-like molecule. Cell 69:927

    PubMed  Google Scholar 

  28. Cyster KG, Shatton D, Williams AF (1991) The dimensions of the T lymphocyte glycoprotein leukosialin γ: identification of linear protein epitopes that can be modified by glycosylation. EMBO J 10:893

    PubMed  Google Scholar 

  29. Williams AF (1991) Out of equilibrium. Nature 352:473

    PubMed  Google Scholar 

  30. Watt SM, Katz FE, Davis L, Capellaro D, Gordon MY, Tindle RW, Greaves MF (1987) Expression of HPCA-1 and HLA-DR antigen on growth factor-dependent and stroma-dependent colony-forming cells. Br J Haematol 66:153

    PubMed  Google Scholar 

  31. Gordon MY, Clarke D, Atkinson J, Greaves MF (1990) Hemopoietic progenitor cells binding to stromal microenvironment in vitro. Exp Hematol 18:837–842

    PubMed  Google Scholar 

  32. Saitoh O, Wang WC, Lotan R, Fukuda M (1992) Differential glycosylation and cell surface expression of lysosomal membrane glycoproteins in sublines of a human colon cancer exhibiting distinct metastatic potentials. J Biol Chem 267:5700

    PubMed  Google Scholar 

  33. Baumhueter S, Singer MS, Henzel W, Hemmerich S, Renz M, Rosen SD, Lasky LA (1993) Binding of L-selection to the vascular sialomucin CD34. Science 262:436–438

    PubMed  Google Scholar 

  34. Majdic O, Stöckl J, Pickl WF, Bohuslav J, Strobl H, Scheinecker C, Stockinger H, Knapp W (1994) Signalling and induction of enhanced cytoadhesiveness via the hematopoietic progenitor cell surface molecule CD34. Blood 83:1226–1234

    PubMed  Google Scholar 

  35. Fackler MJ, Civin CI, May WSS (1992) Up-regulation of surface CD34 is associated with protein kinase C-mediated hyperphosphorylation of CD34. J Biol Chem 267:17540–17546

    PubMed  Google Scholar 

  36. Mentzer SJ, Remold O'Donnell E, Crimmins MAV, Bierer BE, Rosen FS, Burakoff SJ (1987) Sialophorin, a surface sialoglycoprotein-defective Wiskott-Aldrich syndrome, is involved in human T-lymphocyte proliferation. J Exp Med 165:1383

    PubMed  Google Scholar 

  37. Nong YH, Remold O'Donnell E, Lebien TU, Remold HG (1989) A monoclonal antibody to sialophorin (CD43) induced homotypic adhesion and activation of human monocytes. J Exp Med 170:259

    PubMed  Google Scholar 

  38. Rosenkranz AR, Majdic O, Stöckl J, Pickl W, Stockinger H, Knapp W (1993) Induction of neutrophil homotypic adhesion via sialophorin (CD43), a surface sialogylcoprotein restricted to haemopoietic cells. Immunology 80:431–438

    PubMed  Google Scholar 

  39. Howell SM, Molgaard HV, Greaves MF, Spurr NK (1991) Localization of the gene coding for the haemopoietic stem cell antigen CD34 to chromosome 1q32. Hum Genet 87:625

    PubMed  Google Scholar 

  40. Caux C, Facre C, Saeland S, Duvert V, Mannoni P, Durand I, Aubry JP, de Vries JE (1989) Sequential loss of CD34 and class-II MHC antigens on purified cord blood hemopoietic progenitors cultured with IL-3: characterization of CD34-, HLA-DR + cells. Blood 74:1287

    PubMed  Google Scholar 

  41. Okumura N, Tsuji K, Nakahata T (1992) Changes in cell surface antigen expression during proliferation and differentiation of human erythroid progenitors. Blood 80:642–650

    PubMed  Google Scholar 

  42. Sutherland HJ, Eaves CJ, Eaves AC, Dragowska W, Lansdorp PM (1989) Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro. Blood 74:1563–1570

    PubMed  Google Scholar 

  43. Burn TC, Satterthwaite AB, Tenen DG (1992) The human CD34 hematopoietic stem cell antigen promoter and a 3′ enhancer direct hematopoietic expression in tissue culture. Blood 80:3051–3059

    PubMed  Google Scholar 

  44. Gunji Y, Nakamura M, Hagiwara T, Hayakawa K, Matsushita H, Osawa H, Nagayoshi K, Nakauchi H, Yanagisawa M, Miura Y (1992) Expression and function of adhesion molecules on human hematopoetic stem cells: CD34 + LFA-1-cells are more primitive than CD34 + LFA-1 + cells. Blood 80:429–436

    PubMed  Google Scholar 

  45. Liesveld JL, Winslow JM, Frediani KE, Ryan DH, Abboud CN (1993) Expression of integrins and examination of their adhesive function in normal and leukemic hematopoietic cells. Blood 81:112–121

    PubMed  Google Scholar 

  46. Teixidó J, Hemler ME, Greenberger JS, Anklesaria P (1992) Role of β1 and β2 integrins in the adhesion of human CD34hi stem cells to bone marrow stroma. J Clin Invest 90:358–367

    PubMed  Google Scholar 

  47. Satterthwaite ABBT Le Beau MM, Tenen DG (1992) Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. Genomics 12:788–794

    PubMed  Google Scholar 

  48. He XY, Antao VP, Basilia D, Marx JC, Davis BR (1992) Isolation and molecular characterization of the human CD34 gene. Blood 79:2296–2302

    PubMed  Google Scholar 

  49. Melotti P, Ku DH, Calabretta B (1994) Regulation of the expression of the hematopoietic stem cell antigen CD34: role of c-myb. J Exp Med 179:1023–1028

    PubMed  Google Scholar 

  50. Ness SA, Kowenz-Leutz E, Casini T, Graf T, Leutz A (1993) Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types. Genes Dev 7:749–759

    PubMed  Google Scholar 

  51. He XY, Cockerill PN, Cen D, Davis BR (1994) Transcriptional regulation and chromatin structure of the human CD34 gene promoter region. Blood 83:1822–1830

    PubMed  Google Scholar 

  52. Satterthwaite AB, Borson R, Tenen DG (1990) Regulation of the gene for CD34, a human hematopoietic stem cell antigen, in KG-1 cells. Blood 75:2299–2304

    PubMed  Google Scholar 

  53. Terstappen LWMM, Huang S, Picker LJ (1992) Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow. Blood 79:666–677

    PubMed  Google Scholar 

  54. Smith G, Kitchens RL (1989) Phenotypic heterogeneity of TDT+ cells in the blood and bone marrow: implications for surveillance of residual leukemia. Blood 74:312–319

    PubMed  Google Scholar 

  55. Nadler LM, Anderson KC, Marti G, Bates M, Park E, Daley JF, Schlossman SF (1983) B4, a human B lymphocyte-associated antigen expressed on normal mitogen-activated and malignant B lymphocytes. J Immunol 131:244

    PubMed  Google Scholar 

  56. Drexler HG, Thiel E, Ludwig WD (1993) Acute myeloid leukemias expressing lymphoid-associated antigens: diagnostic incidence and prognostic significance. Leukemia 7:489–498

    PubMed  Google Scholar 

  57. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G (1989) B-cell antigens: CD19. In: Knapp W et al. (eds) Leukocyte typing. IV: White cell differentiation antigens. Oxford University Press, Oxford, pp 34–36

    Google Scholar 

  58. Knapp W, Majdic O, Maurer D, Gschwandtler E, Strobl H (1992) Antigenic analysis of CD34 + human haemopoietic progenitor cells. In: Abrahams NG, Marks P, Konwalinka G, Sachs L, Tavassoli M (eds) Intercept, Andover, pp 277–290 Molecular biology of haematopoiesis, vol 3.

    Google Scholar 

  59. Mason D, Cordell J, Tse A, van Dongen J, van Noesel C, Micklem K, Pulford K, Valensi F, Comans-Bitter W, Borst J (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B-cells. J Immunol 147:2474–2482

    PubMed  Google Scholar 

  60. Hermanson GG, Eisenberg D, Kincade PW, Wall R (1988) B29: a member of the immunoglobulin gene superfamily exclusively expressed on beta-lineage cells. Proc Natl Acad Sci USA 85:6890–6894

    PubMed  Google Scholar 

  61. Haynes BF, Denning SM, Singer KH, Kurtzberg J (1989) Ontogeny of T-cell precursors: a model for the initial stages of human T-cell development. Immunol Today 10:87–91

    PubMed  Google Scholar 

  62. Barcena A, Muench AO, Galy AHM, Cupp J, Roncarolo MG, Philips JH, Spits H (1993) Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: CD7 expression in the early stages of T- and myeloid cell development. Blood 82:3401–3414

    PubMed  Google Scholar 

  63. Knapp W (1987) Serology of myeloid leukemias. Cancer Rev 8:66–90

    Google Scholar 

  64. Willière G, Strockinger HUK, Majdic O, Schnabl E, Lutz D, Bettelheim P, Knapp W (1990) Terminal deoxynucleotide transferase and CD7 expression in acute myeloid leukemias are not associated with a high frequency of immunoglobulin and/or T cell receptor gene rearrangement. Leukemia 4:278–281

    PubMed  Google Scholar 

  65. Haynes BF, Martin ME, Hay HH, Kurtzberg J (1988) Early events in human T cell ontogeny: phenotypic characterization and immunohistologic localization of T cell precursors in early human fetal tissues. J Exp Med 168:1061–1080

    PubMed  Google Scholar 

  66. Kurtzberg J, Denning SM, Nycum LM, Singer KH, Haynes BF (1989) Immature human thymocytes can be driven to differentiate into nonlymphoid lineages by cytokines from thymic epithelial cells. Proc Natl Acad Sci USA 86:7575–7579

    PubMed  Google Scholar 

  67. Grümayer ER, Griesinger F, Hummell DS, Brunning RD, Kersey JH (1991) Identification of novel B-lineage cells in human fetal bone marrow that coexpress CD7. Blood 77:64

    PubMed  Google Scholar 

  68. Griffin JD, Linch D, Sabbath K, Larcom P, Schlossman SF (1984) A monoclonal antibody reactive with normal and leukemic human myeloid progenitor cells. Leuk Res 8:521

    PubMed  Google Scholar 

  69. Andrews RG, Singer JW, Bernstein ID (1989) Precursors of colony-forming cells in humans can be distinguished from colony-forming cells by expression of the CD33 and CD34 antigens and light scatter properties. J Exp Med 169:1721–1731

    PubMed  Google Scholar 

  70. Robertson MJ, Soiffer RJ, Freedman AS, Rabinowe SL, Anderson KC, Ervin TJ, Murray C, Dear K, Griffin JD, Nadler LM (1992) Human bone marrow depleted of CD33-positive cells mediates delayed but durable reconstitution of hematopoiesis: clinical trial of MY9 monoclonal antibody-purged autografts for treatment of acute myeloid leukemia. Blood 79:2229–2236

    PubMed  Google Scholar 

  71. Russa VFL, Griffin JD, Kessler SW, Cutting MA, Knight RD, Blattler WA, Lambert JM, Wright DG (1992) Effects of anti-CD33-blocked ricin immunotoxin on the capacity of CD34 + human marrow cells to establish in vitro hematopoiesis in long-term marrow cultures. Exp Hematol 20:442–448

    PubMed  Google Scholar 

  72. Muench MO, Cupp J, Polakoff J, Roncarolo MG (1994) Expression of CD33, CD38, and HLA-DR on CD34 + human fetal liver progenitors with a high proliferative potential. Blood 83:3170–3181

    PubMed  Google Scholar 

  73. Andrews RG, Torok-Storb B, Bernstein ID (1983) Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies. Blood 62:124

    PubMed  Google Scholar 

  74. Andrews RG, Takahashi M, Segal GM, Powell JS, Bernstein ID, Singer JW (1986) The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. Blood 68:1030–1035

    PubMed  Google Scholar 

  75. Modderman PW (1989) New clusters: CD29/CDw49, CD47, CD51, CD55, and CD61. In: Knapp W et al. (eds) Leukocyte typing. IV: White cell differentiation antigens. Oxford University Press, Oxford, pp 1017–1019

    Google Scholar 

  76. Debili N, Issaad C, Masse JM, Guichard J, Katz A, Breton-Gorius J, Vainchenker W (1992) Expression of CD34 and platelet glycoproteins during human megakaryocytic differentiation. Blood 80:3022–3035

    PubMed  Google Scholar 

  77. Saeland S, Duvert V, Caux C, Pandrau D, Favre C, Vallé A, Durand I, Charbord P, de Vries J, Banchereau J (1992) Distribution of surface-membrane molecules on bone marrow and cord blood CD34 + hematopoietic cells. Exp Hematol 20:24–33

    PubMed  Google Scholar 

  78. Köller U, Bettelheim P, Majdic O, Lansdorp PM, Tetteroo PAT, Knapp W (1987) Expression of blood group H antigen on blast cells in leukemias and CML blast crisis. In: Grignani F, Martelli MF, Mason D (eds) Genotypic, phenotypic and functional aspects of haematopoiesis. Raven, New York, pp 109–115

    Google Scholar 

  79. Sieff C, Bicknell D, Caine G, Robinson J, Lam G, Greaves MF (1982) Changes in cell surface antigen expression during hemopoietic differentiation. Blood 60:703–713

    PubMed  Google Scholar 

  80. Papayannopoulou T, Nakamoto B (1993) Peripheralization of hemopoietic progenitors in primates treated with anti-VLA4 integrin. Proc Natl Acad Sci USA 90:9374–9378

    PubMed  Google Scholar 

  81. Bühring HJ, Müller CA, Letarte M, Gougos A, Saalmüller A, van Agthoven AJ, Busch FW (1991) Endoglin is expressed on a subpopulation of immature erythroid cells of normal human bone marrow. Leukemia 5:841–847

    PubMed  Google Scholar 

  82. Villeval JL, Testa U, Vinci G, Tonthat H, Bettaieb A, Titeux M, Cramer P, Edelman L, Rochant H, Breton-Gorius J (1985) Carbonic anhydrase I is an early specific marker of normal human erythroid differentiation. Blood 66:1162

    PubMed  Google Scholar 

  83. Bettaieb A, Farace F, Mitjavila MT, Mishal Z, Dokhelar MC, Tursz T, Breton-Gorius J, Vainchenker W, Kieffer N (1988) Use of a monoclonal antibody (GA3) to demonstrate lineage-restricted O-glycosylation on leukosialin during terminal erythroid differentiation. Blood 71:1226–1233

    PubMed  Google Scholar 

  84. Loken MR, Shah VO, Dattilio KL, Civin CI (1987) Flow cytometric analysis of human bone marrow. I. Normal erythroid development. Blood 69:255–263

    PubMed  Google Scholar 

  85. Lansdorp PM, Sutherland HJ, Eaves CJ (1990) Selective expression of CD45 isoforms on functional subpopulations of CD34 + hemopoietic cells from human bone marrow. J Exp Med 172:363–366

    PubMed  Google Scholar 

  86. Mayani H, Dragowska W, Lansdorp PM (1993) Characterization of functionally distinct subpopulations of CD34 + cord blood cells in serum-free long-term cultures supplemented with hemopoietic cytokines. Blood 82:2664–2672

    PubMed  Google Scholar 

  87. Knapp W, Strobl H, Majdic O (1994) Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis. Cytometry 18:187–198

    PubMed  Google Scholar 

  88. Campana D, Thompson J, Amlot P, Brown S, Janossy G (1987) The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol 138:648–653

    PubMed  Google Scholar 

  89. Rani SM Do, Catovsky D (1988) Differential expression of CD3 and CD22 in leukemic cells according to whether tested in suspension or fixed on slides. Hematol Pathol 2:73–80

    PubMed  Google Scholar 

  90. van Dongen J, Adriaanse H, Hooijkaas H (1988) Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood 71:603–612

    PubMed  Google Scholar 

  91. Janossy G, Cpustan-Smith E, Campana D (1989) The reliability of cytoplasmic CD3 and CD22 antigen expression in the immunodiagnosis of acute leukemia: a study of 500 cases. Leukemia 3:170–178

    PubMed  Google Scholar 

  92. Dörken B, Moldenhauer G, Pezzutto A, Schwartz R, Feller A, Kiesel S, Nadler L (1986) HD39 (B3), a B lineage-restricted antigen whose cell surface expression is limited to resting and activated human B lymphocytes. J Immunol 136:4470–4476

    PubMed  Google Scholar 

  93. Mason D, Stein H, Gerdes J, Pulford K, Ralfkiaer E, Falini B, Erber W, Michaelm K, Gatter K (1987) Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells. Blood 69:836–842

    PubMed  Google Scholar 

  94. van der Schoot C, von dem Borne A, Tetteroo P (1987) Characterization of myeloid leukemias by antibodies with an emphasis on antibodies. Acta Hematol 78:32–40

    Google Scholar 

  95. van der Schoot C, Daams G, Pinkster J, Vet R, von dem Borne A (1990) Monoclonal antibodies against myeloperoxidase are valuable immunological reagents for the diagnosis of acute myeloid leukemia. Br J Haematol 74:173–178

    PubMed  Google Scholar 

  96. Vainchenker W, Villeval J, Tabilio E, Matamis H, Karanakis G, Guichard J, Henri A, Vernant J, Rochant H, Breton Gorius J (1988) Immunophenotype of leukemic blasts with small peroxidase-positive granules detected by electron microscopy. Leukemia 2:274–281

    PubMed  Google Scholar 

  97. Catovsky D, Matutes E, Buccheri V, Shetty V, Hanslip J, Yoshida N, Morilla R (1991) A classification of acute leukaemia for the 1990s. Ann Hematol 62:16–21

    PubMed  Google Scholar 

  98. Knapp W, Majdic O, Strobl H (1993) Flow-cytometric analysis of intracellular myeloperoxidase and lactoferrin in leukemia diagnosis. In: Thiel E, Ludwig WD (eds) (Rec Res Cancer Res) Springer, Berlin Heidelberg New York, pp 32–40

    Google Scholar 

  99. Strobl H, Takimoto M, Majdic O, Fritsch G, Scheinecker C, Höcker P, Knapp W (1993) Myeloperoxidase expression in CD34 + normal human hematopoietic cells. Blood 82:2069–2078

    PubMed  Google Scholar 

  100. Majdic O, Liszka K, Lutz D, Knapp W (1981) Myeloid differentiation antigen defined by a monoclonal antibody. Blood 58:1127

    PubMed  Google Scholar 

  101. Majdic O, Bettelheim P, Stockinger H, Aberer W, Liszka K, Lutz D, Knapp W (1984) M2, a novel myelomonocytic cell surface antigen and its distribution on leukemic cells. Int J Cancer 33:617

    PubMed  Google Scholar 

  102. Knapp W, Majdic O, Blanchard D, Chia W, Feizi T, Gvimesi E, Hiarabayashi Y, Howard CJ, Kniep B, Thompson JS (1994) CDw65 cluster report. In: Schlossman S et al. (eds) Leukocyte typing. V: White cell differentiation antigens. Oxford University Press, Oxford

    Google Scholar 

  103. Delwel R, Bot F, Knapp W, Löwenber B (1987) VIM-2, a candidate monoclonal antibody for purging autologous bone marrow grafts in acute myeloblastic leukemia. Bone Marrow Transplant 2:149

    PubMed  Google Scholar 

  104. Macher BA, Buiehler J, Scudder P, Knapp W, Feizi T (1988) A novel carbohydrate differentiation antigen on fucoganglio-sides of human myeloid cells recognized by monoclonal antibody VIM2. J Biol Chem 163:10186–10191

    Google Scholar 

  105. Lis H, Sharon N (1991) Lectin-carbohydrate interactions. Curr Op Struct Biol 1:741–749

    Google Scholar 

  106. Terstappen LWMM, Huang S, Safford M, Lansdorp PM, Loken MR (1991) Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34 + CD38 — progenitor cells. Blood 77:1218–1227

    PubMed  Google Scholar 

  107. Briddell R, Broudy VC, Bruno E, Brandt JE, Srour EF, Hoffman R (1992) Further phenotypic characterization and isolation of human hematopoietic progenitor cells using a monoclonal antibody to the c-kit receptor. Blood 79:3159–3167

    PubMed  Google Scholar 

  108. Sutherland HJ, Lansdorp PM, Henkelman DH, Eaves AC, Eaves CL (1990) Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proc Natl Acad Sci USA 87:3584–3588

    PubMed  Google Scholar 

  109. Verfaillie C, Blakolmer K, McGlave P (1990) Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectivity to irradiated bone marrow stroma. J Exp Med 172:509–520

    PubMed  Google Scholar 

  110. Rusten LS, Jacobsen SEW, Kaalhus O, Veiby OP, Funderud S, Smeland EB (1994) Functional differences between CD38- and DR- subfractions of CD34 + bone marrow cells. Blood 84:1473–1481

    PubMed  Google Scholar 

  111. Brandt J, Baird N, Lu L, Srour E, Hoffman R (1988) Characterization of a human hematopoietic progenitor cell capable of forming blast cell-containing colonies in vitro. J Clin Invest 82:1017

    PubMed  Google Scholar 

  112. Craig W, Kay R, Cutler RL, Lansdorp PM (1993) Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med 177:1331–1342

    PubMed  Google Scholar 

  113. Péault B, Weissman IL, Baum C, McCune JM, Tsukamoto A (1991) Lymphoid reconstitution of the human fetal thymus in SCID mice with CD34 + precursor cells. J Exp Med 174:1238–1286

    Google Scholar 

  114. Murray L, Chen B, Galy A, Chen S, Tushinski R, Uchida N, Negrin R, Tricot G, Jagannath S, Vesole D (1995) Enrichment of human hematopoietic stem cell activity in the CD34 + Thy + Lin-subpopulation from mobilized peripheral blood. Blood 85:368–378

    PubMed  Google Scholar 

  115. Verfaillie C, Hurley R, Bhatia R, McCarthy JB (1994) Role of bone marrow matrix in normal and abnormal hematopoiesis. Crit Rev Oncol Hematol 16:201–224

    PubMed  Google Scholar 

  116. Hogg N, Landis RC (1993) Adhesion molecules in cell interactions. Curr Op Immunol 5:383–390

    Google Scholar 

  117. Springer TA (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76:301–314

    PubMed  Google Scholar 

  118. Louache F, Debili N, Marandin A, Coulombel L, Vainchenker W (1994) Expression of CD4 by human hematopoietic progenitors. Blood 84:3344–3355

    PubMed  Google Scholar 

  119. Verfaillie CM, McCarthy JB, McGlave PB (1991) Differentiation of primitive human multipotent hematopoietic progenitors into single-lineage clonogenic progenitors is accompanied by alterations in their interaction with fibronectin. J Exp Med 174:693–703

    PubMed  Google Scholar 

  120. Ryan DH, Nuccie BL, Abboud CN (1992) Inhibition of human bone marrow lymphoid progenitor colonies by antibodies of VLA itegrins. J Immunol 149:3759–3764

    PubMed  Google Scholar 

  121. Stockinger H, Gadd SJ, Eher R, Majdic O, Schreiber W, Kasinrek W, Strass B, Schnabl E, Knapp W (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31. J Immunol 145:3889–3897

    PubMed  Google Scholar 

  122. Vaporciyan AA, DeLisser HM, Yan HC, Mendiguren II, Thom SR, Jones ML, Ward PA, Albelda SM (1989) Involvement of platelet-endothelial cell adhesion molecule-1 in neutrophil recruitment in vivo. Science 262:1580–1582

    Google Scholar 

  123. Muller WA, Weigl SA, Deng X, Philips DM (1993) PECAM-1 is required for transendothelial migration of leukocytes. J Exp Med 178:449–460

    PubMed  Google Scholar 

  124. Rosenstein Y, Park JK, Hahn WC, Rosen FS, Bierer BE, Burakoff SJ (1991) CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1. Nature 354:233

    PubMed  Google Scholar 

  125. Ardman B, Sikorski MA, Staunton DE (1992) CD43 interferes with T-lymphocytes adhesion. Proc Natl Acad Sci USA 89:5001–5005

    PubMed  Google Scholar 

  126. Stamenkovic I, Aruffo A, Amiot M, Seed B (1991) The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO J 10:343–348

    PubMed  Google Scholar 

  127. Günthert U, Hofmann M, Rudy W, Reber S, Zöller M, Haußmann I, Matzku S, Wenzel A, Ponta H, Herrlich P (1991) A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 65:13–24

    PubMed  Google Scholar 

  128. Dougherty GJ, Landsdorp PM, Cooper DL, Humphries RK (1991) Molecular cloning of CD44R1 and CD44R2, two novel isoforms of the human CD44 lymphocyte “homing” receptor expressed by hemopoietic cells. J Exp Med 174:1–5

    PubMed  Google Scholar 

  129. Miyake K, Medina KL, Hayashi SI, Ono S, Hamaoka T, Kincade PW (1990) Monoclonal antibodies to Pgp-1/CD44 block lympho-hemapoiesis in long-term bone marrow cultures. J Exp Med 171:477–488

    PubMed  Google Scholar 

  130. Morimoto K, Robin E, LeBousse-Kerdiles MC, Li Y, Clay D, Jasmin C, Smadja-Joffe F (1994) CD44 mediates hyaluronan binding by human myeloid KG1A and KG1 cells. Blood 83:657–662

    PubMed  Google Scholar 

  131. Verfaillie CM, Benis A, Iida J, McGlave P, McCarthy JB (1994) Adhesion of committed human hematopoietic progenitors to synthetic peptides from the C-terminal heparin binding domain of fibronectin: cooperation between the integrin α4β1 and the CD44 adhesion receptor. Blood 84:1802–1811

    PubMed  Google Scholar 

  132. Witte O (1990) Steel locus defines new multipotent growth factor. Cell 63:5–6

    PubMed  Google Scholar 

  133. Moore MAS (1991) Clinical implications of positive and negative hematopoietic stem cell regulators. Blood 78:1–19

    PubMed  Google Scholar 

  134. Ashman LK, Cambarei AC, To LB, Levinsky RJ, Juttner CA (1991) Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bore marrow. Blood 78:30–37

    PubMed  Google Scholar 

  135. Bühring HJ, Ullrich A, Schaudt K, Müller CA, Busch FW (1991) The product of the proto-oncogene c-kit (P145 c-kit) is a human bone marrow surface antigen of hemopoietic precursor cells which is expressed on a subset of acute non-lymphoblastic leukemic cells. Leukemia 5:854–860

    PubMed  Google Scholar 

  136. Papayannopoulou T, Brice M, Broudy VC, Zsebo KM (1991) Isolation of c-kit receptor-expressing cells from bone marrow, peripheral blood, fetal liver: functional properties and composite antigenic profile. Blood 78:1403–1412

    PubMed  Google Scholar 

  137. Yamaguchi Y, Gunji Y, Nakamura M, Hayakawa K, Maeda M, Osawa H, Nagayoshi K, Kasahara T, Suda T (1993) Expression of c-kit mRNA and protein during the differentiation of human hematopoietic progenitor cells. Exp Hematol 21:1233–1238

    PubMed  Google Scholar 

  138. Strobl H, Takimoto M, Majdic O, Höcker P, Knapp W (1992) Antigenic analysis of human haemopoietic progenitor cells expressing the growth factor receptor c-kit. Br J Haemotol 82:287–294

    Google Scholar 

  139. Tjonford GE, Veiby OP, Steen R, Egeland T (1993) T lymphocyte differentiation in vitro from adult human prethymic CD34 + bone marrow cells. J Exp Med 177:1531–1539

    PubMed  Google Scholar 

  140. Matos ME, Schnier GS, Beecher MS, Ashman LK, Williams DE, Caligiuri MA (1993) Expression of a functional c-kit receptor on a subset of natural killer cells. J Exp Med 178:1079–1084

    PubMed  Google Scholar 

  141. Gunji Y, Nakamura M, Osawa H, Nagayoshi K, Nakaauchi H, Miura Y, Yanagisawa M, Suda T (1993) Human primitive hematopoietic progenitor cells are more enriched in kit-low cells than in kit-high cells. Blood 82:3283–3289

    PubMed  Google Scholar 

  142. Bennett BD, Wang Z, Kuang W-J, Wang A, Groopman JE, Goeddel DV, Scadden DT (1994) Cloning and characterization of HTK, a novel transmembrane tyrosine kinase of the EPH subfamily. J Biol Chem 269:14211–14218

    PubMed  Google Scholar 

  143. Neubauer A, Fiebeler AGD O'Bryan JP, Schmidt CA, Barckow P, Serke S, Siegert W, Snoddgrass HR, Huhn D, Liu ET (1994) Expression of axl, a transforming receptor tyrosine kinase, in normal and malignant hematopoiesis. Blood 84:1931–1941

    PubMed  Google Scholar 

  144. Small D, Levenstein M, Kim E, Carow C, Amin S, Rockwell P, Witte L, Burroww C, Ratajczak MZ, Gewirtz AM (1994) STK-1, the human homolog of flk-2/flt-3, is selectively expressed in CD34 + human bone marrow cells and is involved in the proliferation of early progenitor/stem cells. Proc Natl Acad Sci USA 91:459 463

    PubMed  Google Scholar 

  145. Matthews W, Jordan CT, Wiegand GW, Pardoll D, Lemischka IR (1991) A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell 65: 1143–1152

    PubMed  Google Scholar 

  146. Rosnet O, Schiff C, Pebusque MJ, Marchetto S, Tonnelle C, Toiron Y, Birg F, Birnbaum D (1993) Human flt-3/flk2 gene: cDNA cloning and expression in hematopoietic cells. Blood 82:1110–1119

    PubMed  Google Scholar 

  147. Hannum C, Culpepper J, Campbell D, McClanahan T, Zurawski S, Bazan JF, Kastelein R, Hudak S, Wagner J, Mattson J (1994) Ligand for flt3/flk2 receptor tyrosine kinase regulates growth of hematopoietic stem cells and is encoded by variant RNAs. Nature 368:643–648

    PubMed  Google Scholar 

  148. Lyman SD, James LJL, Brasel K, de Vries P, Escobar SS, Downey H, Splett RR, Beckmann MP, McKenna HJ (1994) Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells. Blood 83:2795–2801

    PubMed  Google Scholar 

  149. Sato N, Caux C, Kitamura T, Watanabe Y, Arai K-I, Banchereau J, Miyamjima A (1993) Expression and factor-dependent modulation of the interleukin-3 receptor subunits on human hematopoietic cell. Blood 82:752–761

    PubMed  Google Scholar 

  150. Sato N, Sawada K, Koizumi K, Tarumi T, Ieko M, Yasukouchi T, Yamaguchi M, Takahashi TA, Sekiguchi S, Koike T (1993) In vitro expansion of human peripheral blood CD34 + cells. Blood 82:3600–3609

    PubMed  Google Scholar 

  151. Wognum A, Westerman Y, Visser TP, Visser GW (1994) Distribution of receptors for granulocyte-macrophage colony-stimulating factor on immature CD34 + bone marrow cells, differentiating monomyeloid progenitors, and mature blood cell subsets. Blood 84:764–774

    PubMed  Google Scholar 

  152. Wognum A, van Gils FCJ, Wagemaker G (1993) Flow cytometric detection of receptors for interleukin-6 on bone marrow and peripheral blood cells of humans and rhesus monkeys. Blood 81:2036–2043

    PubMed  Google Scholar 

  153. Wognum AW, Krystal G, Eaves CJ, Eaves AC, Lansdorp PM (1992) Increased erythropoietin-receptor expression on CD34- positive bone marrow cells from patients with chronic leukemia. Blood 79:642–649

    PubMed  Google Scholar 

  154. McClanahan T, Dalrymple S, Barkett M, Lee F (1993) Hematopoietic growth factor receptor genes as markers of lineage commitment during in vitro development of hematopoietic cells. Blood 81:2903–2915

    PubMed  Google Scholar 

  155. Vigon I, Momon JPCL, Mitjavila M-T, Tambourin P, Gissellbrecht S, Souyri M (1992) Molecular cloning and characterization of MPL, the human homolog of the v-mpl oncogen: identification of a member of the hematopoietic growth factor receptor superfamily. Proc Natl Acad Sci USA 89:5604–5644

    Google Scholar 

  156. de Sauvage FJ, Hass PE, Spencer SD, Malloy BEGA, Spencer SA, Darbonne WA, Henzel WJWS, Kuang WJ, Oles KL, Hultgren B (1994) Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-mpl ligand. Nature 369:533–538

    PubMed  Google Scholar 

  157. Methia N, Louache F, Vainchenker WFW (1993) Oligodeoxy-nucleotides antisense to the proto-oncogene c-mpl specifically inhibit in vitro megakaryocytopoiesis. Blood 82:1395–1401

    PubMed  Google Scholar 

  158. Debili N, Wendling F, Cosman D, Titeux M, Fglorindo C, Dusante-Fourt I, Schooley K, Methia N, Chardon M, Nador R (1995) The mpl receptor is expressed in the megakaryotic lineage from late progenitors to platelets. Blood 85:391–401

    PubMed  Google Scholar 

  159. Craig JIO, Turner ML, Parker AC (1992) Peripheral blood stem cell transplantation. Blood Rev 6:59–67

    PubMed  Google Scholar 

  160. Siena S, Bregni M, Brando B, Ravagni F, Bonadonna G, Gianni AM (1989) Circulation of CD34 + hematopoietic stem cells in the peripheral blood of high-dose cyclophosphamide-treated patients: enhancement by intravenous recombinant human granulocyte-macrophage colony-stimulating factor. Blood 74:1905–1914

    PubMed  Google Scholar 

  161. Tjonnford GE, Steen R, Ebensen SA, Thorsby E, Egeland T (1994) Characterization of CD34 + peripheral blood cells from healthy adults mobilized by recombinant human granulocyte colony-stimulating factor. Blood 84:2795–2801

    PubMed  Google Scholar 

  162. Dreger P, Haferlach T, Eckstein V, Jacobs S, Suttorp M, Löffler H, Müller-Ruchholtz W, Schmitz N (1994) G-CSF-mobilized peripheral blood progenitor cells for allogenic transplantation: safety, kinetics of mobilization, and composition of the graft. Br J Haematol 87:609–613

    PubMed  Google Scholar 

  163. Fritsch G, Fischmeister G, Haas OA, Peters C, Gadner H, Strobl H, Höcker P, Kurz M (1994) Peripheral blood hematopoietic progenitor cells of cytokine-stimulated healthy donors as an alternative for allogeneic transplantation. Blood 83:3421

    Google Scholar 

  164. Andrews RG, Bartelmez SH, Knitter GH, Myerson D, Bernstein ID, Appelbaum FR, Zsebo KM (1992) A c-kit ligand, recombinant human stem cell factor, mediates revers- ible expansion of multiple CD34 + colony forming cell types in blood and marrow of baboons. Blood 80:920 927

    PubMed  Google Scholar 

  165. Tong J, Gordon MS, Srour EF, Cooper RJ, Orazi A, McNiece I, Hoffman R (1993) In vivo administration of recombinant methionyl human stem cell factor expands the number of human marrow hematopoietic stem cells. Blood 82:784–791

    PubMed  Google Scholar 

  166. Caux C, Dezutter Dambuyant C, Schmitt D, Banchereau J (1992) GM-CSF and TNF-a cooperative in the generation of dendritic Langerhans cells. Nature 360:258

    PubMed  Google Scholar 

  167. Lotzova E, Savary CA, Champlin RE (1993) Genesis of human oncolytic natural killer cells from primitive CD34 + CD33-bone marrow progenitors. J Immunol 150:5263–5269

    PubMed  Google Scholar 

  168. Miller JS, Verfaillie C, McGlave P (1992) The generation of human natural killer cells from CD34 +/DR-primitive progenitors in long-term bone marrow culture. Blood 80:2182–2187

    PubMed  Google Scholar 

  169. Saeland S, Caux C, Favre C, Aubry JP, Mannoni P, Pebusque MJ, Gentilhomme O, Otsuka T, Yokota T, Arai N (1988) Effects of recombinant human interleukin-3 on CD34-enriched normal hematopoietic progenitors and on myeloblastic leukemia cells. Blood 72:1580–1588

    PubMed  Google Scholar 

  170. Galey N, Verma S, Barcena A, Spits H (1993) Precursors of CD3 + CD4 + CD8 + cells in the human thymus are defined by expression of CD34. Delineation of early events in human thymic development. J Exp Med 178:391–401

    PubMed  Google Scholar 

  171. Serke S, Säuberlich S, Abe Y, Huhn D (1991) Analysis of CD34-positive hemopoietic progenitor cells from normal human adult peripheral blood: flow-cytometrical studies and in vitro colony (CFU-GM, BFU-E) assays. Ann Hematol 62:45–53

    PubMed  Google Scholar 

  172. Ema H, Suda T, Nakauchi H, Nakamura Y, Iwama A, Imagawa S, Akutsu M, Kano Y, Kato S, Yabe M (1991) Multipotent and committed CD34 + cells in bone marrow transplantation. Jpn J Cancer Res 82:547–552

    PubMed  Google Scholar 

  173. Thoma SJ, Lamping CP, Ziegler BL (1994) Phenotype analysis of hematopoietic CD34 + cell populations derived from human umbilical cord blood using flow cytometry and cDNA-polymerase chain reaction. Blood 83:2103–2114

    PubMed  Google Scholar 

  174. Kato K, Radbruch A (1993) Isolation and characterization of CD34 + hematopoietic stem cells human peripheral blood by high-gradient magnetic cell sorting. Cytometry 14:384–392

    PubMed  Google Scholar 

  175. Inaba T, Shimazaki C, Ashihara E, Ohkawa K, Oku N, Gotoh H, Fujita N, Tsuji H, Nakagawa M (1992) Two-color flow cytometric analysis of CD34-positive peripheral blood stem cells mobilized by recombinant human granulocyte-colony stimulating factor for autotransplantation. In: Worthington-Wight DA, Gee AP, Gross S (eds) Advances in bone marrow purging and processing. Wiley & Liss, New York, USA 377:561–567

    Google Scholar 

  176. Loken MR, Shah VO, Dattilo KL, Civin CI (1987) Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood 70:1316–1324

    PubMed  Google Scholar 

  177. Pontvert-Delucq S, Breton-Gorius J, Schmitt C, Bailou C, Guichard J, Najman A, Lemoine FM (1993) Characterization and functional analysis of adult human bone marrow cell subsets in relation to B-lymphoid development. Blood 82:417–429

    PubMed  Google Scholar 

  178. Saeland S, Duvert V, Pandrau D, Caux C, Durand I, Wrighton N, Wideman J, Lee F, Banchereau J (1991) Interleukin-7 induces the proliferation of normal human B-cell precursors. Blood 78:2229–2238

    PubMed  Google Scholar 

  179. Kerst JM, Slaper-Cortenbach ICM, Bome AEGKvd, Schoot CEvd, Oers RHJv (1992) Combined measurement of growth and differentiation in suspension cultures of purified human CD34-positive cells enables a detailed analysis of myelopoiesis. Exp Hematol 20:1188–1193

    PubMed  Google Scholar 

  180. Teofili L, Lovino MS, Sica S, Pierelli L, Menichella G, De Stefano V, Rumi C, Leone G (1994) Characterization of peripheral blood CD34 + progenitor cells mobilized with chemotherapy and granulocyte colony-stimulating factor. Exp Hematol 22:990–995

    PubMed  Google Scholar 

  181. Fukuda T, Okamura S, Shimoda K, Takamtsu Y, Inaba S, Harada M, Niho Y (1994) Predominance of myeloid antigens in CD34-positive peripheral blood stem cells over those in bone marrow after administration of granulocyte colony-stimulating factor. Eur J Haematol 52:201–206

    PubMed  Google Scholar 

  182. Haylock DN, To LB, Dowse TL, Juttner CA, Simmons PJ (1992) Ex vivo expansion and maturation of peripheral blood CD34 + cells into the myeloid lineage. Blood 80:1405–1412

    PubMed  Google Scholar 

  183. Bender JG, Unverzagt K, Walker DE, Lee W, Smith S, Williams S, Epps DEV (1994) Phenotypic analysis and characterization of CD34 + cells from normal human bone marrow, cord blood, peripheral blood, and mobilized peripheral blood from patients undergoing autologous stem cell transplantation. Clin Immunol Immunopathol 70:10–18

    PubMed  Google Scholar 

  184. Haas R, Ehrhardt R, Witt B, Goldschmidt H, Hohaus S, Pförsich M, Ehrlich H, Färber L, Hunstein W (1993) Auto-grafting with peripheral blood stem cells mobilized by sequential interleukin-3/granulocyte-macrophage colony-stimulating factor following high-dose chemotherapy in non-Hodgkin's lymphoma. Bone Marrow Transplant 12:643–649

    PubMed  Google Scholar 

  185. Hohaus S, Goldschmidt H, Ehrhardt R, Haas R (1993) Successful autografting following myeloablative conditioning therapy with blood stem cells mobilized by chemotherapy plus rhG-CSF. Exp Hematol 21:508–514

    PubMed  Google Scholar 

  186. LeBien TW, Wörmann B, Villabianca JG, Che-Leung L, Steinberg LM, Shah VO, Loken MR (1990) Multiparameter flow cytometric analysis of human fetal bone marrow B cells. Leukemia 4:354–358

    PubMed  Google Scholar 

  187. Bernstein ID, Leary AG, Andrews RG, Ogawa M (1991) Blast colony-forming cells and precursors of colony-forming cells detectable in long-term marrow culture express the same phenotype (CD33-CD34+). Exp Hematol 19:680–682

    PubMed  Google Scholar 

  188. Kinniburgh D, Russell NH (1993) CD34 — positive cells and subpopulations in human umbilical cord blood and bone marrow. Bone Marrow Transplant 12:489–494

    PubMed  Google Scholar 

  189. Gabianelli M, Sargiacomo M, Pelosi E, Testa U, Isacchi G, Peschle C (1990) “Pure” human hematopoietic progenitors: permissive action of basic fibroblast growth factor. Science 249:1561–1564

    PubMed  Google Scholar 

  190. Brugger W, Bross K, Frisch J, Dem P, Weber B, Mertelsmann R, Kanz L (1992) Mobilization of peripheral blood progenitor cells by sequential administration of interleukin-3 and granulocyte-macrophage colony-stimulating factor following polychemotherapy with etoposide, ifosfamide, and cisplatin. Blood 79:1193–1200

    PubMed  Google Scholar 

  191. Ericson SG, Benoit NE, Mills LE, Fanger MW (1994) The effect of recombinant human interleukin-3 an recombinant human granulocyte-macrophage colony-stimulating factor on Fcgamma receptor expression and antibody-dependent cellular cytoxicity of hematopoietic progenitor cells during in vitro myeloid maturation. Exp Hematol 22:283–289

    PubMed  Google Scholar 

  192. Lansdorp PM, Dragowska W (1992) Long-term erythropoiesis from constant numbers of CD34 + cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow. J Exp Med 175:1501–1509

    PubMed  Google Scholar 

  193. Andrews RG, Singer JW, Bernstein ID (1990) Human hematopoietic precursors in long-term culture: single CD34 + cells that lack detectable T cell, B cell and myeloid cell antigens produce multiple colony-forming cells when cultured with marrow stromal cells. J Exp Med 172:355–358

    PubMed  Google Scholar 

  194. Keating A, Powell J, Takahashi M, Singer JW (1984) The genertion of human long-term marrow cultures from marrow depleted of la (HLA-DR)-positive cells. Blood 64:1159–1162

    PubMed  Google Scholar 

  195. Leary G, Ogawa M (1987) Blast cell colony assay for umbilical cord blood and adult bone marrow progenitors. Blood 69:953

    PubMed  Google Scholar 

  196. Leary AG, Zeng HQ, Clark SC, Ogawa M (1992) Growth factor requirements for survival in Go and entry into the cell cycle of primitive human hemopoietic progenitors. Proc Natl Acad Sci USA 89:4013–4017

    PubMed  Google Scholar 

  197. Lu L, Walker D, Broxmeyer HE, Hoffman R, Hu W, Walker E (1987) Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with my10 and major histocompability class-II monoclonal antibodies. J Immunol 139:1823

    PubMed  Google Scholar 

  198. Snoeck HW, Lardon F, Lenjou M, Nys G, Van Boekstaele DR, Peetersman ME (1993) Differential regulation of the expression of CD38 and human leukocyte antigen-DR on CD34 + hematopoietic progenitor cells by interleukin-4 and interferon-g. Exp Hematol 21:1480–1486

    PubMed  Google Scholar 

  199. Srour EF, Brandt JE, Briddell RA, Leemhuis T, Vanbesien K, Hoffman R (1991) Human CD34 + HLA-DR-bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis. Blood Cells 17:287–295

    PubMed  Google Scholar 

  200. Cicuttini FM, Welch K, Boyd AW (1994) Characterization of CD34 + HLA -DR-CD38+ and CD34+ HLA-DR-CD38-progenitor cells from human umbilical cord blood. Growth Factors 10:127–134

    PubMed  Google Scholar 

  201. Huang S, Terstappen LWMM (1994) Lymphoid and myeloid differentiation of single human CD34+, HLA-DR+, CD38-hematopoietic stem cells. Blood 83:1515–1526

    PubMed  Google Scholar 

  202. Tong J, Hoffman R, Siena S, Srour EF, Bregni M, Gianni AM (1994) Characterization and quantitation of primitive hematopoietic progenitor cells present in peripheral blood autografts. Exp Hematol 22:1016–1024

    PubMed  Google Scholar 

  203. Cardoso AA, Li ML, Batard P, Hatzfeld A, Brown EL, Levesque JP, Sookdeo H, Panteme B, Sansilvestri P, Clark SC (1993) Release from quiescence of CD34 + CD38-human umbilical cord blood cells reveals their potential to engraft adults. Proc Natl Acad Sci USA 90:8707–8711

    PubMed  Google Scholar 

  204. Reuss-Borst MA, Bühring HJ, Klein G, Müller CA (1992) Adhesion molecules on CD34 hematopoietic cells in normal human bone marrow and leukemia. Ann Hematol 65: 169–174

    PubMed  Google Scholar 

  205. Liesveld JL, Winslow JM, Kempski MC, Ryan DH (1991) Adhesive interactions of normal and leukemic human CD34 + myeloid progenitors: role of marrow stromal, fribroblast, and cytomatrix components. Exp Hematol 19:63–70

    PubMed  Google Scholar 

  206. Watt SM, Williamson J, Genevier H, Fawcett J, Simmons DL, Hatzfeld A, Nesbitt SA, Coombe DR (1993) The heparin-binding PECAM-1 adhesion molecule is expressed by CD34 + hematopoietic precursor cells with early myeloid and B-myeloid cell phenotypes. Blood 82:2649–2663

    PubMed  Google Scholar 

  207. Lewinsohn DM, Nagler A, Ginzton N, Greenberg P, Butcher EC (1990) Hematopoietic progenitor cell expression of the H-CAM (CD44) homing-associated adhesion molecule. Blood 75:589–595

    PubMed  Google Scholar 

  208. Kerst JM, Sanders JB, Slaper-Cortenbach ICM, Doorakkers MC, Hooibrink B, Oers RHJv, Bome AEGKvd, Schoot CEvd (1993) a4b1 and a5b1 are differentially expressed during myelopoiesis and mediate the adherence of human CD34 + cells to fibronectin in an activation-dependent way. Blood 81:344–351

    PubMed  Google Scholar 

  209. Terstappen LWMM, Nguyen M, Lazarus HM, Medof ME (1992) Expression of the DAF (CD55) and CD59 antigens during normal hematopoietic cell differentiation. J Leuk Biol 52:652–660

    Google Scholar 

  210. Lanza F, Bi S, Castoldi G, Goldman JM (1993) Abnormal expression of N-CAM (CD56) adhesion molecule on myeloid and progenitor cells from chronic myeloid leukemia. Leukemia 7:1570–1575

    PubMed  Google Scholar 

  211. Dworzak MN, Fritsch G, Buchinger P, Fleischer C, Printz D, Zellner A, Schöllhammer A, Steiner G, Ambros PF, Gadner H (1994) Flow cytometric assessment of human MIC2 expression in bone marrow, thymus, and peripheral blood. Blood 83:415–425

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. W. Knapp

    Present address: Institute of Immunology, University of Vienna, Borschkegasse 8a, A-1090, Vienna, Austria

Authors and Affiliations

  1. Institute of Immunology, University of Vienna, Borschkegasse 8a, A-1090, Vienna, Austria

    O. Majdic

  2. Institute of Immunology, Vienna International Research Cooperation Center at Sandoz Forschungsinstitut, Brunnerstrasse 59, A-1235, Vienna, Austria

    H. Strobl, C. Scheinecker & C. Bello-Fernandez

Authors
  1. W. Knapp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Strobl
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Scheinecker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Bello-Fernandez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. Majdic
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Work supported byFonds zur Förderung der wissenschaftlichen Forschung and the COMAC-BIO European Concerted Action Program

Rights and permissions

Reprints and permissions

About this article

Cite this article

Knapp, W., Strobl, H., Scheinecker, C. et al. Molecular characterization of CD34+ human hematopoietic progenitor cells. Ann Hematol 70, 281–296 (1995). https://doi.org/10.1007/BF01696614

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01696614

Keywords

Search

Navigation