Summary
Using the structural gene for the ribosomal protein L3 from Saccharomyces cerevisiae as a probe, we isolated a homologous fragment from genomic DNA of Schizosaccharomyces pombe. Analysis of the plasmid carrying this fragment by hybridization selection and 2D-electrophoresis revealed a 31 kDa ribosomal protein. Transformation of the vector pDB248x containing this fragment into Schizosaccharomyces pombe leads to an increased level of mRNA suggesting that we have cloned the entire and actively transcribed gene.
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Beach D, Nurse P (1981) Nature (London) 290:140–142
Beach D, Piper M, Nurse P (1982) Nature (London) 187:326–329
Coddington A, Fluri R (1977) Mol Gen Genet 158:93–100
Erdmann VA, Huysmans E, Vandenberghe A, de Wachter R (1983) Nucleic Acids Res 11:105–133
Fried HM, Warner JR (1981) Proc Natl Acad Sci USA 78:238–242
Fried HM, Pearson NJ, Kim CH, Warner JR (1981) J Biol Chem 256:10176–10183
Fried HM, Warner JR (1984) Organization and expression of eukaryotic ribosomal protein genes. In: Stein J, Stein G (eds) Recombinant DNA and cell proliferation. Academic Press, New York London, pp 169–192
Laemmli UK (1970) Nature (London) 227:680–685
Lake JA (1983) Prog Nucleic Acid Res Mol Biol 30:163–194
Leer JR, van Raamsdonk-Duin MMC, Molenaar CMTh, Cohen LH, Mager WH, Planta RJ (1982) Nucleic Acids Res 10:5869–5878
Nurse P (1975) Nature (London) 256:547–551
Nurse P (1985) Trends Genet 1:51–55
Otaka E, Hiyo K, Itoh T (1983) Mol Gen Genet 191:519–524
Perry RP, Dudov KP (1984) Cell 37:457–468
Rosbash M, Blank D, Fahrner K, Herford L, Ricciardi R, Roberts B, Ruby S, Wollford J (1979) Methods Enzymol 68:454–469
Schultz LD, Friesen JD (1983) J Bacteriol 155:8–14
Southern EM (1975) J Mol Biol 98:503–517
Teem JL, Abovich N, Käufer NF, Schwindinger WF, Warner JR, Levy A, Woolford J, Leer JR, van Raamsdonk-Duin MMC, Mager WH, Planta RJ, Schultz L, Friesen JD, Fried H, Rosbash M (1984) Nucleic Acids Res 12:8295–8312
Warner JR, Gorenstein Ch (1978) Methods Cell Biol 20:45–60
Woolford JL, Rosbash M (1979) Nucleic Acids Res 6:2483–2497
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Nischt, R., Thüroff, E. & Käufer, N.F. Molecular cloning of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe . Curr Genet 10, 365–370 (1986). https://doi.org/10.1007/BF00418408
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DOI: https://doi.org/10.1007/BF00418408