Summary
We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.
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Communicated by K. Wolf
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Grey, M., Brendel, M. Rapid and simple isolation of DNA from agarose gels. Curr Genet 22, 83–84 (1992). https://doi.org/10.1007/BF00351746
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DOI: https://doi.org/10.1007/BF00351746