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Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein

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Abstract

Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 aminoacid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.

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Authors and Affiliations

  1. Laboratoire de Génétique, URA CNRS 542, Avenue des Facultés, F-33405, Talence Cedex, France

    Matthieu Régnacq & Hélian Boucherie

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  1. Matthieu Régnacq
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  2. Hélian Boucherie
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Communicated by P. P. Slonimski

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Régnacq, M., Boucherie, H. Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein. Curr Genet 23, 435–442 (1993). https://doi.org/10.1007/BF00312631

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  • DOI: https://doi.org/10.1007/BF00312631

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