Summary
The ultrastructural localisation of class II and macrophage antigens has been sought in the intimal layer of the human synovium. The cells described as type A in early morphological studies are shown to express both class II antigens and two markers of the macrophage/monocyte lineage of cells, OKM1 and MAB 24. The morphological type B cells were found to express none of these antigens. The findings are consistent with the idea that the synovial lining comprises two cell types, bone-marrow derived macrophages and mesenchymal fibroblasts.
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References
Barland P, Novikoff AB, Hamerman D (1962) Electron microscopy of the human synovial membrane. J Cell Biol 14:207–220
Krey PR, Cohen AS (1973) Fine structural analysis of rabbit cells. 1. The normal synovium and changes in organ culture. Arthritis Rheum 16:324–340
Ghadially FN (1983) The fine structure of synovial joints. Butterworths, London
Forre O, Thoen J, Lea T, Dubloug JH, Mellbye OJ, Nativg JB, Pahle J, Solheim BG (1982) In situ characterisation of mononuclear cells in rheumatoid arthritis tissues, using monoclonal antibodies. Scand J Immunol 16:315–319
Klareskog L, Forsum U, Kabelitz D, Ploen L, Sundstrom C, Nilsson K, Wigren A, Wigzell H (1982) Immune functions of human synovial cells. Arthritis Rheum 25:488–501
Burmester GR, Locher P, Winchester RJ, Dimitriu-Bona A, Kalden JR, Mohr W (1983) The tissue architecture of synovial membranes in inflammatory joint diseases. 1. The localisation of the major cell populations as detected by monoclonal regents directed to Ia and monocyte-macrophage antigens. Rheumatol Int 3:173–181
Lindblad S, Klareskog L, Hedfors E, Forsum U, Sundstrom C (1983) Phenotypic characterisation of synovial tissue cells in different types of synovitis. Arthritis Rheum 26:1321–1332
Palmer DG, Selvendran Y, Allen C, Revell PA, Hogg N, (1985) Features of synovial membrane identified with monoclonal antibodies. Clin Exp Immunol 59:529–583
Hogg N, Palmer DG, Revell PA (1985) Intimal cells of the synovial membrane identidied by monoclonal antibodies. B J Rheumatol 24:79 (abstr)
Shiozawa S, Shiozawa K, Fujita T (1983) Presence of HLA-DR antigens on synovial type A and B cells: an immunoelectron microscopic study in rheumatoid arthritis, osteoarthritis and normal traumatic joints. Immunology 50:587–594
Iguchi T, Kurosaka M, Ziff M (1986) An electronmicroscopic study of HLA-DR and monocyte/macrophage staining cells in the rheumatoid synovial membrane. Arthritis Rheum 29:600–613
Hogg N, Selvendran Y (1985) An anti-human monocyte/macrophage marker reacting most strongly with macrophages in lymphoid tissue. Cell Immunol 92:247–253
Breard JM, Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 24:1943–1948
Sanchez-Madrid F, Nagy JA, Robbins F, Simon P, Springer TA (1983) A human leucocyte differentiation antigen family with distinct alpha subunits and a common beta subunit. J Exp Med 158:1785–1793
Vilella R, Yague J, Gallant MT, Vives J (1983) The efficacy of hybridoma technology in studying surface antigens in T and B lymphocytes. Immunologia 2:58–60
Alonzo ADR, Jarequemada D, Ollier W, Navarrette C, Festenstein H (1984) alpha and beta chain polymorphisms of HLA-DR4 antigens. Dis Markers 2:119–125
Van Ewijk W, Coffman RC, Weissman IL (1980) Immunoelectron microscopy of cell surface antigens: a quantitative analysis of antibody binding after different fixation protocols. Histochem J 12:349–361
Mapp PI (1987) Studies on the intimal cells of the synovial membrane. PhD thesis, University of London
Lalor PA, Mapp PI, Hall PA, Revell PA (1987) Proliferative activity of cells in the synovium as demonstrated by the monclonal antibody Ki67. Rheumatol Int 7:183–186
Henderson B, Revell PA, Edwards JCW (1988) Viewpoint. Synovial lining cell hyperplasia in rheumatoid arthritis: dogma and fact. Ann Rheum Dis 47 (in press)
Theofilopoulos AN, Carson DA, Tavassoli M, Slavin SF, Spears WC, Jensen FB, Vaugh JH (1980) Evidence for the presence of receptors for C3 and IgG Fc on human synovial cells. Arthritis Rheum 23:1–9
Graabaek PM (1982) Ultrastructural evidence for two distinct types of synoviocytes in rat synovial membrane. Ultrastruct Res 78:321–339
Graabaek PM (1984) Characteristics of the two types of synoviocytes in the rat synovial membrane. An ultrastructural study. Lab Invest 50:690–702
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Mapp, P.I., Revell, P.A. Ultrastructural characterisation of macrophages (type A cells) in the synovial lining. Rheumatol Int 8, 171–176 (1988). https://doi.org/10.1007/BF00270456
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DOI: https://doi.org/10.1007/BF00270456