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Photosynthetically active suspension cultures of potato spindle tuber viroid infected tomato cells as tools for studying viroid — host cell interaction

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Abstract

Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media containing 3% sucrose, but during continuous photo-mixotrophic culture in low sucrose media (1% sucrose), the level of PSTVd accumulation decreased. Photoautotrophic cell suspensions could be established with uninfected, but not with viroid infected tomato cells. As compared to uninfected cells, PSTVd infected cells grew slowly, were morphologically different in size and shape, and formed tight cell aggregates. Electronmicroscopy showed that starch accumulation in chloroplasts, deformation of the chloroplast envelope and irregular plasmalemmasomes at the cell membrane were associated with PSTVd infection.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-benzylaminopurine

CEVd:

citrus exocortis viroid

CSVd:

chrysanthemum stunt viroid

PSTVd:

potato spindle tuber viroid

TMV:

tobacco mosaic virus

phc:

photoheterotrophic cell culture

mcc:

photomixotrophic cell culture

pcc:

photoautotrophic cell culture

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Author notes

  1. Angela Barth

    Present address: Department of Molecular and Cellular Physiology, Beckman Center, Stanford University Medical Center, Stanford, California, USA

Authors and Affiliations

  1. Arbeitsbereich Genetik, Institut für Allgemeine Botanik und Botanischer Garten, Universität Hamburg, Ohnhorststraße 18, D-22609, Hamburg, Germany

    Susanne Stöcker, Marie-Christine Guitton, Angela Barth & Hans-Peter Mühlbach

Authors
  1. Susanne Stöcker
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  2. Marie-Christine Guitton
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  3. Angela Barth
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  4. Hans-Peter Mühlbach
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Communicated by H. Lörz

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Stöcker, S., Guitton, MC., Barth, A. et al. Photosynthetically active suspension cultures of potato spindle tuber viroid infected tomato cells as tools for studying viroid — host cell interaction. Plant Cell Reports 12, 597–602 (1993). https://doi.org/10.1007/BF00232806

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  • DOI: https://doi.org/10.1007/BF00232806

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