Abstract
Media were developed to regenerate shoots from leaf pieces of tamarillo (Cyphomandra betacea (Cav.) Sendtner). Shoots were derived via organogenesis and could be easily rooted and transferred to the growth chamber. Transgenic tamarillo plants were produced using the binary vector pKIWI110 in the avirulent Agrobacterium strain LBA4404. All transgenic plants were kanamycin resistant and some plants expressed the β D-glucuronidase (gusA) reporter gene and were chlorsulfuron resistant. Molecular evidence for transformation was obtained using PCR (polymerase chain reaction) and Southern hybridization. Inheritance of the transgenic phenotypes was demonstrated in seedling progeny.
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References
Atkinson RG, Candy CJ, Gardner RC (1990) NZ J. Crop Hortic. Sci. 18: 153–156
Atkinson RG, Gardner RC (1991) Plant Cell Rep. 10: 208–212
Cuozzo M, O'Connell KM, Kaniewski W, Fang R-X, Chua N-H, Tumer NE (1988) Bio/Technology 6: 549–557
Dawes SN, Pringle GJ (1983) In: Wratt GS, Smith HC (eds) Plant Breeding in New Zealand. Butterworths, Wellington, pp 123–138
Dellaporta SL, Wood J, Hicks JB (1983) Plant Mol. Biol. Rep. 1: 19–21
Eagles RM, Gardner RC, Forster RLS (1990) Nucleic Acid Res. 18: 7166
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158
Gasser CS, Fraley RT (1989) Science 244: 1293–1299
Godwin I, Todd G, Ford-Lloyd B, Newbury HJ (1991) Plant Cell Rep. 9: 671–675
Hemenway C, Fang R-X, Kaniewski WK, Chua N-H, Tumer NE (1988) EMBO J. 7: 1273–1280
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) Nature 303: 179–180
Hoekema A, Huisman MJ, Molendijk L, van den Elzen PJM, Cornelissen BJC (1989) Bio/Technology 7: 273–278
Janssen B-J, Gardner RC (1989) Plant Mol. Biol. 14: 61–72
Jefferson RA (1987) Plant Mol. Biol. Rep. 5: 387–405
Lu C-Y, Nugent G, Wardley-Richardson T, Chandler SF, Young R, Dalling MJ (1991) Bio/Technology 9: 864–868
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
Powell Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Science 232: 738–743
Schmidt R, Willmitzer L (1988) Plant Cell Rep. 7: 583–586
van den Elzen PJM, Huisman MJ, Posthumus-Lutke Willink D, Jongedijk E, Hoekema A, Cornelissen BJC (1989) Plant Mol. Biol. 13: 337–346
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Communicated by J. M. Widholm
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Atkinson, R.G., Gardner, R.C. Regeneration of transgenic tamarillo plants. Plant Cell Reports 12, 347–351 (1993). https://doi.org/10.1007/BF00237433
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DOI: https://doi.org/10.1007/BF00237433