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Stable transformation of Eustoma grandiflorum by particle bombardment

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Abstract

Explants (7.5±2.5 mm) cut from stems and roots of 3-week-old Eustoma grandiflorum Grise, (lisianthus) cv. Glory White seedlings were bombarded with plasmid pBI221, which harbors the uidA gene encoding β-glucuronidase (GUS) driven by the cauliflower mosaic virus (CaMV) 35S promoter. More than 800 blue spots of GUS-expressing cells were observed per 90 explants. Explants bombarded with pARK22 harboring the bar gene encoding phosphinothricin acetyltransferase driven by the CaMV 35S promoter were selected for bialaphos resistance. Putative transgenic plants were obtained about 3 months after bombardment. Southern blot analysis of putative transgenic plants revealed the presence of the bar gene in their genome.

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Authors and Affiliations

  1. Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan, , , , , , JP

    M. Takahashi, K. Irifune & H. Morikawa

  2. Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003, Japan, , , , , , JP

    M. Nishihara & S. Yamamura

  3. Agriculture Corporation Federation, Nagano Prefecture, Susaka 381-0012, Japan, , , , , , JP

    S. Nishizawa

Authors
  1. M. Takahashi
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  2. M. Nishihara
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  3. S. Yamamura
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  4. S. Nishizawa
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  5. K. Irifune
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  6. H. Morikawa
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Received: 10 April 1996 / Revision received: 7 November 1997 / Accepted: 22 November 1997

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Takahashi, M., Nishihara, M., Yamamura, S. et al. Stable transformation of Eustoma grandiflorum by particle bombardment. Plant Cell Reports 17, 504–507 (1998). https://doi.org/10.1007/s002990050432

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  • DOI: https://doi.org/10.1007/s002990050432

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