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Reversible variations in the methylation pattern of carrot DNA during somatic embryogenesis

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Abstract

Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The level of transcription of the relevant DNA fragments during embryogenesis was investigated by Northern blotting. One of the two probes did not show signals in any of the developmental stages whereas the other showed developmentally regulated expression. This fact suggests a novel strategy for cloning developmentally expressed functions.

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Abbreviations

mC:

5-methyl-cytosine

CpG:

dinucleotide cytosine-guanine

CpNpG:

trinucleotide cytosine-any base-guanine

HPLC:

high performance liquid chromatography

2,4-D:

2,4-dichlorophenoxyacetic acid

6-BAP:

6-benzyl-aminopurine

References

  • Busslinger M, de Boer S, Wright S, Groveld IG, Flavell RA (1983) Nucl. Acid Res. 8: 3559–3569

    Google Scholar 

  • Choi J, Sung ZR (1984) Plant Mol.Biol.Rep. 2: 19–23

    Google Scholar 

  • Giuliano G, Rosellini R, Terzi M (1983) Plant Cell Rep. 2: 216–218

    Google Scholar 

  • Giuliano G, Skolnick PA (1988) Plant Physiol. 86: 7–9

    Google Scholar 

  • Gruenbaum Y, Naveh-Many T, Cedar H, Razin A (1981) Nature 292: 860–862

    Google Scholar 

  • Hepburn AG, Belanger FC, Mattheis JR (1987) Devel. Genet. 8: 475–493

    Google Scholar 

  • Lo Schiavo F, Pitto L, Giuliano G, Torti G, Nuti Ronchi V, Marazziti D, Vergara R, Orselli S, Terzi M (1989) Theor. Appl. Genet. 77: 325–331.

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning — a laboratory manual. Cold Spring Harbor Laboratory

  • Murray MG, Thompson WF (1980) Nucl. Acid Res. 8: 4321–4325

    Google Scholar 

  • Pitto L, Lo Schiavo F, Terzi M (1985) Proc. Nat. Acad. Sci. US 82: 2799–2803

    Google Scholar 

  • Razin A, Szyf M, Fafri T, Roll M, Giloh H, Scarpa S, Carotti D, Cantoni GL (1986) Proc. Nat. Acad. Sci US 83: 2827–2831

    Google Scholar 

  • Sung ZR, Okimoto R (1983) Proc. Nat. Acad. Sci. US 80: 2661–2665

    Google Scholar 

  • Thomas TL, Wilde D (1985) in: Somatic Embryogenesis (Terzi M, Pitto L, Sung ZR eds.) IPRA, Rome pp.75–85

    Google Scholar 

  • Vanyushin BF, Belozersky AN, Kokurina NA, Kadirova DX (1968) Nature 218: 1066–1067.

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Mutagenesi e Differenziamento, CNR, Via Svezia 10, 2A, I-56124, Pisa, Italy

    Rosaria Vergara, Fulvia Verde & Letizia Pitto

  2. Istituto Internazionale di Genetica e Biofisica, CNR, Napoli, Italy

    Fiorella Lo Schiavo

  3. Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli, Napoli, Italy

    Mario Terzi

Authors
  1. Rosaria Vergara
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  2. Fulvia Verde
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  3. Letizia Pitto
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  4. Fiorella Lo Schiavo
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  5. Mario Terzi
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Communicated by H. Lörz

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Vergara, R., Verde, F., Pitto, L. et al. Reversible variations in the methylation pattern of carrot DNA during somatic embryogenesis. Plant Cell Reports 8, 697–700 (1990). https://doi.org/10.1007/BF00272097

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  • DOI: https://doi.org/10.1007/BF00272097

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