Skip to main content
SpringerLink
Log in

Automated DNA-image cytometry: a prognostic tool in infiltrating bladder carcinoma?

  • Published:
World Journal of Urology Aims and scope Submit manuscript

Summary

In a retrospective study, paraffin-embedded cystectomy specimens obtained from 46 patients with bladder cancer (stage pT1 — pT4a, pN0, pN2) were analysed for tumor DNA ploidy and proliferation using automated image cytometry (LEYTAS). In 41 cases, DNA ploidy could be measured. Estimation of proliferation was possible in 26 tumors. The number of cells with a DNA content higher than 5C could be calculated in 38 of the tumors. All these three parameters are shown to correlate with patient outcome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bandhauer K, Nemeth Th (1983) Die prognostische Relevanz des primären Differenzierungsgrades und des Erythrozyten-Adhärenz-Testes (SRCA) bei oberflächlichen Blasenkarzinomen. Akt Urol 14:119–122

    Google Scholar 

  2. Bergmann S, Javadpur N (1978) The cell surface antigen A, B or 0(H) as an indicator of malignant potential in stage A bladder carcinoma. J Urol 119:49–51

    Google Scholar 

  3. Coon JS, Schwartz D, Summers JL, Miller AW, Weinstein RS (1986) Flow cytometric analysis of deparaffinized nuclei in urinary bladder carcinoma. Comparison with cytogenetic analysis. Cancer 57:1594–1601

    Google Scholar 

  4. Driel-Kulker AMJV, Mesker WE, Velzen IV, Tanke HJ, Feichtinger J, Ploem JS (1985) Preparation of monolayer smears from paraffinembedded tissue for image cytometry. Cytometry 6:268–272

    Google Scholar 

  5. Driel-Kulker AMJv, Ploem JS (1982) The use of LEYTAS in analytical and quantitative cytology. IEEE transactions on biomedical engineering, vol. BME-29, no 2, pp 92–100

  6. England HR, Paris AMI, Blandy JP (1981) The correlation of T1 bladder tumour history with prognosis and follow-up requirements. Br J Urol 53:593–597

    Google Scholar 

  7. Farsund T, Hoestmark JG, Laerum OD (1984) Relation between flow cytometry DNA distribution and pathology in human bladder cancer. A report of 69 cases. Cancer 54:1771–1777

    Google Scholar 

  8. Gustafson H, Tribukait B, Esposti PL (1982) The prognostic value of DNA analysis in primary carcinoma in situ of the urinary bladder. Scand J Urol Nephrol 16:141–146

    Google Scholar 

  9. Harker WG, Meyers FJ, Freiha FS, Palmer JM, Shortliffe LD, Hannigan JF, McWhirter KM, Torti FM (1985) Cisplatin, methotrexate and vinblastine (CMV): an elective chemotherapy regimen for metastatic transitional cell carcinoma of the urinary tract. A northern California oncology group study. J Clin Oncol 3:1463–1470

    Google Scholar 

  10. Harmer MH (1978) TNM classification of malignant tumors, 3rd edn. Union Internationale Contre le Cancer (UICC), Geneva

    Google Scholar 

  11. Hedley DW, Riedlander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffinembedded pathological material using flow cytometry. J Histochem Cytochem 31:1333–1335

    Google Scholar 

  12. Heney NM, Proppe K, Prout GR, Griffin PP, Shipley WU (1983) Invasive bladder cancer: Tumour configuration, lymphatic invasion and survival. J Urol 130:895–897

    Google Scholar 

  13. Hope-Stone HF, Oliver RTD, England HR, Blandy JP (1984) T3 bladder cancer: salvage rather than elective surgery after radiotherapy. Urology 24:315–320

    Google Scholar 

  14. Ingen EMv, Verwoerd N, Ploem JS (1980) LEYTAS-2: A hybrid system for the analysis of cytological preparations, using both hardware and software methods. Microscopica Acta [Suppl] 4:73–81

    Google Scholar 

  15. Jacobi GH, Klippel KF, Hohenfellner R (1983) 15 Jahre Erfahrung mit der radikalen Zystektomie ohne präoperative Radiotherapie beim Harnblasenkarzinom. Akt Urol 14:63–69

    Google Scholar 

  16. Jakse G, Rauschmeier H, Lentsch P, Hofstaedter F (1983) Der Tumorinfiltrationstyp. Eine Möglichkeit zur Klassifizierung muskelinfiltrierender Blasentumoren. Akt Urol 14:179–182

    Google Scholar 

  17. Jakse G, Frommhold H, zur Nedden D (1985) Combined radiation and chemotherapy for locally advanced transitional cell carcinoma of the urinary bladder. Cancer 55:1659–1664

    Google Scholar 

  18. Jewett HJ (1952) Carcinoma of the bladder: influence of depth of infiltration on the 5 year results following complete exstirpation of the primary growth. J Urol 67:672–676

    Google Scholar 

  19. Jewett HJ, King LR, Shelley WM (1964) A study of 365 cases of infiltrating bladder cancer: relation of certain pathological characteristics to prognosis after exstirpation. J Urol 92:668–678

    Google Scholar 

  20. Ljungberg B, Stenling R, Roos G (1986) Prognostic value of Deoxyribonucleic Acid content in metastatic renal cell carcinoma. J Urol 136:801–804

    Google Scholar 

  21. Lutzeyer W, Ruebben H, Dahm H (1982) Prognostic parameters in superficial bladder cancer: an analysis of 315 cases. J Urol 127:250–252

    Google Scholar 

  22. Meyer F (1979) Iterative image transformations for automative screening of cervical smears. J Histochem Cytochem 27:128–135

    Google Scholar 

  23. Oljans PJ, Tanke HJ (1986) Flow cytometric analysis of DNA content in bladder cancer: prognostic value of the DNA index with respect to early tumor recurrence in G2 tumours. World J Urol 4:205–210

    Google Scholar 

  24. Skinner DG (1982) Management of invasive bladder cancer: a meticulous pelvic node dissection can make a difference. J Urol 128:34–36

    Google Scholar 

  25. Skinner DG, Lieskovsky G (1984) Contemporary cystectomy with pelvic node dissection compared to preoperative radiation therapy plus cystectomy in management of invasive bladder cancer. J Urol 131:1069–1072

    Google Scholar 

  26. Slack NH, Prout GR (1980) The heterogeneity of invasive bladder cancer carcinoma and different response to treatment. J Urol 123:644–652

    Google Scholar 

  27. Sternberg CN, Yagoda A, Scher HI, Watson RC, Ahmed T, Weiselberg LR, Geller N, Hollander PS, Herr HW, Sogani PC, Morse MJ, Whitmore WF (1985) Preliminary results of M-VAC (Methotrexate, Vinblastine, Doxorubicin and Cis-platin) for transitional cell carcinoma of the urothelium. J Urol 133:403–407

    Google Scholar 

  28. Stoeckle M, Alken P, Jacobi GH, Hohenfellner R (1987) Besitzt die pelvine Lymphadenektomie im Rahmen der radikalen Zystektomie eine therapeutische Bedeutung? 38. Verhandlungsgericht der dt Ges f Urol. Springer, Berlin Heidelberg New York (in press)

    Google Scholar 

  29. Stoeckle M, Alken P, Engelmann U, Jacobi GH, Riedmiller H, Hohenfellner R (1987) Radical cystectomy — often too late? Eur Urol (in press)

  30. Studer UE, Ruchti E, Greiner RM, Zingg EJ (1983) Faktoren, welche die Überlebensrate nach radikaler Zystektomie beeinflussen. Akt Urol 14:70–77

    Google Scholar 

  31. Tanke HJ, Ingen EMv, Ploem JS (1979) Acriflavine-Feulgen Stilbene staining: A procedure for automated cervical cytology with a television based system (LEYTAS). J Histochem Cytochem 27:84–86

    Google Scholar 

  32. Tanke HJ, Ploem JS, Jonas U (1982) Kombinierte Durchflußzytometrie und Bildanalyse zur automatisierten Zytologie von Blasenepithel und Prostata. Akt Urol 13:109–115

    Google Scholar 

  33. Tomasino RM, Orestano F, Morello V (1985) Pathologische Charakteristika des infiltrierenden Blasenkarzinoms mit Bezug zur Metastasierung: Eine vorläufige Auswertung von 59 radikalen Zystektomiepräparaten. Akt Urol 16:218–221

    Google Scholar 

  34. Vindelov LL, Christensen IJ, Nissen NI (1983) A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3:323–327

    Google Scholar 

  35. Wallace DM, Bloom HJG (1976) The management of deeply infiltrating (T3) bladder carcinoma: controlled trial of radical radiotherapy versus preoperative radiotherapy and radical cystectomy (first report). Br J Urol 48:587–594

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Urology, University of Mainz Medical School, Langenbeckstraße 1, D-6500, Mainz, Federal Republic of Germany

    M. Stöckle & R. Hohenfellner

  2. Department of Cytochemistry and Cytometry, Leiden University Medical Center, Leiden, The Netherlands

    H. J. Tanke, W. E. Mesker & J. S. Ploem

  3. Department of Urology, Leiden University Medical Center, Leiden, The Netherlands

    U. Jonas

Authors
  1. M. Stöckle
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Tanke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. E. Mesker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. S. Ploem
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. U. Jonas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. Hohenfellner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by a grant of the Deutsche Forschungsgemeinschaft

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stöckle, M., Tanke, H.J., Mesker, W.E. et al. Automated DNA-image cytometry: a prognostic tool in infiltrating bladder carcinoma?. World J Urol 5, 127–132 (1987). https://doi.org/10.1007/BF00327070

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00327070

Keywords

Search

Navigation