Skip to main content
Log in

Dynamics of nodulation, nitrogen fixation, nitrogen use and biomass yield over time in pot-grown Leucaena leucocephala (Lam.) de Wit

  • Orginal Paper
  • Published:
Biology and Fertility of Soils Aims and scope Submit manuscript

Abstract

The dynamics of nodulation, N2-fixation and N use in Leucaena leucocephala cv. K28 over time was investigated in a screenhouse at 4, 8, 12 and 16 months after planting (MAP) using the 15N-labelling method. Leucaena had a consistently increasing pattern of nodulation, dry biomass and nitrogen yield. A sharp rise in nodulation was observed between 12 and 16 MAP, whereas for biomass, N accumulation and N2-fixation, and N2-fixation, an upward surge occurred between 4 and 12 months. Nodulation, N accumulation, N2-fixation and biomass yield all peaked at 16 MAP. Along with the steady increase in N2-fixation throughout the 16-month growth period, the % N derived from the atmosphere rose from 17.9% to 61.5%, 70.1% and 74%, equivalent to 191, 1623, 2395 and 3385 mg N2 fixed plant-1 at 4, 8, 12 and 16 MAP, respectively. Nitrogen assimilation from soil and fertilizer decreased inversely to the increase in symbiotic nitrogen fixation with time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baker RJ (1980) Multiple comparison tests. Can J Plant Sci 60: 325–327

    Google Scholar 

  • Brewbaker JL (1987) Leucaena: a multipurpose tree genus for tropical agroforestry. In: Steplen HA, Nair PKR (eds) Agroforestry: decade of development. CRAF, Nairobi, pp 289–323

    Google Scholar 

  • Bowen GD (1990) Managing nitrogen fixation by trees. ACIAR Forest Newslett 10: 3–4

    Google Scholar 

  • Danso SKA (1985) Methods of estimating biological nitrogen fixation. In: Ssali H, Keya SO (eds) Biological nitrogen fixation in Africa. Rhizobium Mircen, Nairobi, pp 224–244

    Google Scholar 

  • Dommergues YR (1987) The role of biological nitrogen fixation in agroforestry. In: Stepler HA, Nair PKR (eds) Agroforestry: a decade of development. ICRAF, Nairobi, pp 245–271

    Google Scholar 

  • Duhoux E, Dommergues Y (1985) The use of nitrogen fixing trees in forestry and soil restoration in the tropics. In: Ssali H, Keya SO (eds) Biological nitrogen fixation in Africa, Nairobi Mircen, Kenya, pp 384–400

    Google Scholar 

  • Fried M, Middelboe V (1977) Measurement of amount of nitrogen fixed by a legume crop. Plant Soil 47: 713–715

    Google Scholar 

  • Hansen AP, Pate JS (1987) Comparative growth and symbiotic performance of seedlings of Acacia spp. in defined pot culture and as natural understorey components of a eucalyptus forest ecosystem in S.W., Australia. J Exp Bot 38: 13–25

    Google Scholar 

  • Haque I, Jutzi S (1984) Nitrogen fixation by forage legumes in subSaharan Africa: potentials and limitations. ILCA Buletin 20: 2–13

    Google Scholar 

  • Hardarson G, Danso SKA, Zapata F (1987) Biological nitrogen fixation in field crops. In: Christie BR (ed) Handbook of plant science in agriculture. CRC, pp 165–192

  • Hardy RWF (1977) Rate limiting steps in biological reproductivity. In: Hollander A (ed) Genetic engineering for nitrogen fixation. Plenum, New York, pp 369–399

    Google Scholar 

  • Jensen ES (1991) Evaluation of automated analysis of 15N and total N in plant material and soil. Plant Soil 133: 83–92

    Google Scholar 

  • Kang BT, Wilson GF, Sipkens L (1981) Alley cropping maize (Zea mays L.) and leucaena (Leucaena leucocephala Lam.) in southern Nigeria. Plant Soil 63: 165–179

    Google Scholar 

  • Knowles R (1981) The measurement of nitrogen fixation. In: Gibson AH, Newton WE (eds) Current perspectives in nitrogen fixation. Australian Academy of Science, Canberra, pp 327–399

    Google Scholar 

  • Lawn RJ, Brun WA (1974) Symbiotic nitrogen fixation in soybeans. I. Effect of photosynthetic source-sink manipulations. Crop Sci 14: 11–16

    Google Scholar 

  • NAS (1977) Leucaena: promising forage and tree crop for the tropics. national Academy of Science, Washington DC, 115 pp

    Google Scholar 

  • Rennie RJ, Kemp GA (1984) 15N-determined time course for nitrogen fixation in two cultivars of field beans. Agron J 76: 146–154

    Google Scholar 

  • Roskoski JP, Montano J, Van Kessel C, Castilleia G (1982) Nitrogen fixation by tropical woody legumes. In: Graham PM, Harris SC (eds) Biological nitrogen fixation technology in tropical agriculture. Centro Internacional de Agricultura Tropical. Cali, Colombia, pp 447–454

    Google Scholar 

  • Roskoski JP (1981) Nodulation and N-fixation by Inga jinicuil, a woody legume in coffee plantation. I. Measurement of nodule biomass and field C2H2 reduction rates. Plant Soil 59: 201–206

    Google Scholar 

  • Sanginga N, Bowen GD, Danso SKA (1990) Assessment of genetic variability for N2 fixation between and within provenances of Leucaena leucocephala and Acacia albida estimated by 15N labelling techniques. Plant Soil 127: 169–178

    Google Scholar 

  • SAS (1985) SAS user's guide: basics, SAS Institute, Cary, NC, USA

    Google Scholar 

  • Vance RJ, Heichel GH, Barnes DK, Bryan JW, Johnson LE (1979) Nitrogen fixation, nodule development and vegetative regrowth of alfalfa (Medicago sativa L.) following harvest. Plant Physiol 64: 1–8

    Google Scholar 

  • Venkateswarlu B, Singh RP (1988) Nodulation and nitrogenase activity patterns of Leucaena leucocephata and Gliricidia sepium during early stages of growth. Leucaena Res Rep 9: 55–58

    Google Scholar 

  • Vincent JM (1970) A practical manual for the study of root nodule bacteria. IBP Handbook no. 15 Blackwell Scientific, Oxford

    Google Scholar 

  • Williams WA, Jones MB, Delwiche CC (1977) Clover N-fixation measurement by total N difference and 15N A-values in lysimeters. Agron J 69: 1023–1024

    Google Scholar 

  • Zapata F, Danso SKA, Hardarson G, Fried M (1987) Time course of nitrogen fixation in field grown soybean using nitrogen-15 methodology. Agron J 79: 172–176

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kadiata, B.D., Mulongoy, K. & Isirimah, N.O. Dynamics of nodulation, nitrogen fixation, nitrogen use and biomass yield over time in pot-grown Leucaena leucocephala (Lam.) de Wit. Biol Fertil Soils 20, 163–168 (1995). https://doi.org/10.1007/BF00336553

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00336553

Key words

Navigation