Abstract
Organic N solubilized by NH3(aq) was extracted from 15N-labelled or unlabelled soil, concentrated and added to non-extracted soil, which was incubated under aerobic conditions at 27±1°C. Gross N mineralization, gross N immobilization, and nitrification in soils with or without addition of unlabelled soluble organic N were estimated by models based on the dilution of the NH +4 or NO sup-inf3 pools, which were labelled with 15N at the beginning of incubation. Mineralization of labelled organic N was measured by the appearance of label in the mineral N pool. Although gross N mineralization and gross N immobilization were increased in two soils between day 0 and day 7 following addition of unlabelled organic N solubilized by NH3(aq), there was no increase in net N mineralization. Solubilization of 15N-labelled organic N increased and the 15N enrichment of the soluble organic N decereased as the concentration of NH3(aq) added increased. A constant proportion of approximately one-quarter of the labelled organic N added at different rates to non-extracted soil was recovered in the mineral N pool after an incubation period of 14 days, and the availability ratios calculated from net N mineralization data were 1.1:1 and 2.1:1 for 111 and 186 mg added organic-N kg-1 soil, respectively, indicating that the mineralization of organic N was increased by solubilization.
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References
Barraclough D (1988) Studying mineralization/immobilization turnover in field experiments: the use of nitrogen-15 and simple mathematical analysis. In: Jenkinson DS, Smith KA (eds) Nitrogen efficiency in agricultural soils. Elsevier Applied Science, London, pp 409–417
Bjamason S (1988) Calculation of gross nitrogen immobilization and mineralization in soil. J Soil Sci 39:393–406
Bremner JM, Mulvaney CS (1982) Nitrogen-total. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. Part 2. Chemical and microbiological properties, 2nd edn. Agronomy 9, Am Soc Agron, Madison, Wis, pp 595–624
Burge WD, Broadbent FE (1961) Fixation of ammonia by organic soils. Soil Sci Soc Am Proc 25:199–204
Chen D, Chalk PM, Freney JR (1990) Release of dinitrogen from nitrite and sulphamic acid for isotope ratio analysis of soil extracts containing nitrogen-15 labelled nitrite and nitrate. Analyst 115:365–370
Chen D, Chalk PM, Freney JR, Smith CJ, Luo QX (1995) Estimation of nitrification rates in flooded soils. Microb Ecol 30:269–284
Crawford DM, Chalk PM (1992) Mineralization and immobilization of soil and fertilizer nitrogen with nitrification inhibitors and solvents. Soil Biol Biochem 24:559–568
Gillman GP (1979) A proposed method for the measurement of exchange properties of highly weathered soils. Aust J Soil Res 17:129–139
Guiraud G, Marol C, Thibaud MC (1989) Mineralization of nitrogen in the presence of a nitrification inhibitor. Soil Biol Biochem 21:29–34
He XT, Mulvaney RL, Stevenson FJ, Vanden Heuvel RM (1990) Characterization of chemically fixed liquid anhydrous ammonia in an Illinois Drummer soil. Soil Sci Soc Am J 54:775–780
He XT, Mulvaney RL, Stevenson FJ (1991) Transformations of chemically fixed liquid anhydrous ammonia by soil microorganisms. Biol Fertil Soils 11:145–150
Jenkinson DS, Fox RH, Rayner JH (1985) Interactions between fertilizer nitrogen and soil nitrogen-the so-called ‘priming’ effect. J Soil Sci 36:425–444
Keeney DR, Nelson DW (1982) Nitrogen-inorganic forms. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. Part 2. Chemical and microbiological properties, 2nd edn. Agronomy 9, Am Soc Agron, Madison, Wis, pp 643–698
Kirkham D, Bartholomew WV (1954) Equations for following nutrient transformations in soil, utilizing tracer data. Soil Sci Soc Am Proc 18:33–34
McLaughlin MJ, Fillery IR, Till AR (1992) Operation of the phosphorus, sulphur and nitrogen cycles. In: Gifford RM, Barson MM (eds) Australia's renewable resources: sustainability and global changes. Bureau of Rural Resources Proceedings No 12, Canberra, pp 67–116
Myers RG, Thien SJ (1988) Organic matter solubility and soil reaction in an ammonium and phosphorous application zone. Soil Sci Soc Am J 52:516–522
Norman RJ, Gilmour JT (1987) Utilization of anhydous ammonia fixed by clay minerals and soil organic matter. Soil Sci Soc Am J 51:959–962
Norman RJ, Kurtz LT, Stevenson FJ (1987) Solubilization of soil organic matter by liquid by anhdrous ammonia. Soil Sci Soc Am J 51:809–812
Norman RJ, Gilmour JT, Gale PM (1988) Transformation of organic matter solubilized by anhydrous ammonia. Soil Sci Soc Am J 52:694–697
Payne RW (1987) Genstat 5 reference manual. Clarendon Press, Oxford
Pruden G, Powlson DS, Jenkinson DS (1985) The measurement of 15N in soil and plant material. Fert Res 6:205–218
Sen S, Chalk PM (1994) Solubilization of soil organic N by alkaline-hydrolysing N fertilizers. Fert Res 38:131–139
Sen S, Chalk PM (1995) Biological interactions between soil nitrogen and alkaline-hydrolysing nitrogen fertilizers. Biol Fertil Soils 20:41–48
Smith CJ, Chalk PM, Crawford DM, Wood JT (1994) Estimating gross nitrogen mineralization and immobilization rates in anerobic and aerobic soil suspensions. Soil Sci Soc Am J 58:1652–1660
Stace HCT, Hubble GD, Brewer R, Northcote KH, Sleeman JR, Mulcahy MJ, Hallsworth EG (1968) A handbook of Australian soils. Rellim Technical Publications, Glenside, South Australia
Stehouwer RC, Johnson JW (1991) Soil adsorption interactions of band-injected anhydrous ammonia and potassium chloride fertilizers. Soil Sci Soc Am J 55:1374–1381
Tomasiewicz DJ, Henry JL (1985) The effect of anhydrous ammonia applications on the solubility of soil organic carbon. Can J Soil Sci 65:737–747
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Sen, S., Chalk, P.M. Mineralization of soil organic nitrogen solubilized by aqua ammonia fertilizer. Biol Fertil Soils 23, 93–98 (1996). https://doi.org/10.1007/BF00335824
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DOI: https://doi.org/10.1007/BF00335824