Summary
Biochemical and morphologic studies were carried out on the early effect of hypercholesterolemia on the central nervous system in both metabolically normal and subdiabetic rabbits. Major biochemical difference was the elevation of non-esterified cholesterol in the subdiabetic animals. Histologically, Alzheimer type II astrocytosis was noted in the basal ganglia of the subdiabetic rabbits during the 4th week. The astrocytosis extended into the entire protion of the cerebrum and cerebellum during 2 to 4 months. In the metabolically normal rabbits on the cholesterol diet, on the other hand, similar astrocytic changes were noted after 2 months. Despite the fact that cholesterol crystals had frequently been noted in the capillary walls of the viscera after the 2nd week, similar changes were not observed in the blood vessels of the brains of both the subdiabetic and the metabolically normal rabbits during the 4 months of cholesterol feeding. These observations suggest that cholesterol deposits in the vascular walls are determined by the different rates of lipid metabolism in each individual organ.
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References
Adachi, M., Torii, J., Karvounis, P. C., Volk, B. W.: Alterations of astrocytic organelles in various lipidoses and allied diseases. Acta neuropath. (Berl.)18, 74–83 (1971a).
——, Schneck, L., Volk, B. W.: The fine structure of fetal Tay-Sachs disease. Arch. Path.91, 48–54 (1971b).
————: Fine structue of early Tay-Sachs disease. In: B. W. Volk and S. M. Aronson (eds.): VIth International Symposium on Sphingolipids, Sphingolipidoses and Allied Disorders, p. 1. New York: Plenum Publishing Co. 1972a.
——, Wellmann, K. F., Volk, B. W.: The late phase of central nervous lesions in experimental hypercholesterolemia. J. Neuropath. exp. Neurol.31, 332–345 (1972b).
Adams, E., Hertig, A. T.: Studies on the human corpus luteum. I. Observations on the ultrastructure of development and regression of the luteal cells during the menstrual cycle. J. Cell Biol.41, 696–715 (1969).
Bernhard, W., Rouiller, C.: Close topographical relationship between mitochondria and ergastoplasm of liver cells in a definite phase of cellular activity. J. biophys. biochem. Cytol. (Suppl.)2, 73–78 (1956).
Chiamori, N., Henry, R. J.: Study of the ferric chloride method for determination of total cholesterol and cholesterol esters. Amer. J. clin. Path.31, 305–309 (1959).
Constantinides, P.: Experimental atherosclerosis. New York: Elsevier 1965.
Ellenberg, M.: Diabetic complications with manifest diabetes: Complications as presenting clinical symptoms. J. Amer. med. Ass.183, 926–930 (1963).
Fabrykant, M., Gelfand, M. L.: Symptom-free diabetes in angina pectoris. Amer. J. med. Sci.247, 665–668 (1964).
Feigin, I.: A method for the histochemical differentiation of cholesterol and its esters. J. biophys. biochem. Cytol.2, 213–214 (1956).
Hawk, P. B., Oser, B., Summerson, W. H.: Practical physiological chemistry. Ed. 13, pp. 589 to 590. New York: The Blakiston Co. 1966.
Herman, M. V., Gorlin, R.: Premature coronary artery disease and the preclinical diabetic state. Amer. J. med. Sci.38, 481–484 (1965).
Kremer, G. L., Niemczyk, H., Knick, B.: Coronarsklerose und subklinischer Diabetes mellitus. Schweiz. med. Wschr.97, 735–739 (1967).
Kuntz, A., Sulkin, N. M.: Lesions induced in rabbits by cholesterol feeding with special reference to their origin. Arch. Path.47, 248–260 (1949).
Lever, L. D.: Electron microscopic observations on the adrenal cortex. Amer. J. Anat.97, 409–430 (1955).
Long, J. A., Jones, A. L.: The fine structure of the zona glomerulosa and the zona fasciculata of the adrenal cortex of the opossum. Amer. J. Anat.120, 463–487 (1967).
Naoumenko, J., Feigin, I.: A modification for paraffin sections of the Cajal gold-sublimate stain for astrocytes. J. Neuropath. exp. Neurol.20, 602–604 (1961).
——: A modification for paraffin sections of the silver carbonate stain for microglia. Acta neuropath. (Berl.)2, 402–406 (1963).
Nelson, N.: A photometric adaptation of Somogyi method for determination of glucose. J. biol. Chem.153, 375–380 (1944).
Prończuk, A., Fillios, L. C.: Changes in cholesterol concentrations in rough and smooth endoplasmic reticulum and polysomal profiles in rats fed cholesterol. J. Nutr.96, 46–52 (1968).
Van Slyke, D. D., Plazin, J.: The preparation of extracts for plasma lipids free from watersoluble contaminants. Clin. chim. Acta12, 46–54 (1965).
Volk, B. W., Wellmann, K. F.: Experimental atherosclerosis in normal and subdiabetic rabbits. II. Long term studies. Atherosclerosis14, 331–339 (1971).
Wahlbery, F.: The intravenous glucose tolerance test in atherosclerotic disease with special reference to obesity, hypertension, diabetic heredity and cholesterol values. Acta med. scand.171, 1–7 (1962).
Wang, C. I., Strauss, L., Adlersberg, D.: Experimental xanthomatosis in the rabbit. Arch. Path.63, 416–422 (1957).
Wellmann, K. F., Brancato, P., Lazarus, S. S., Volk, B. W.: Rabbit beta-cell ultrastructure and insulin radio-immunoassay in experimental subdiabetes. Arch. Path.84, 251–263 (1967a).
—, Volk, B. W.: Renal changes in experimental hypercholesterolemia in normal and in subdiabetic rabbits. I. Short term studies. Lab. Invest.22, 36–49 (1970a).
——: Experimental atherosclerosis in normal and subdiabetic rabbits. I. Short term studies. Arch. Path.90, 206–217 (1970b).
——: Renal changes in experimental hypercholesterolemia in normal and in subdiabetic rabbits. II. Long term studies. Lab. Invest.24, 144–155 (1971a).
——: The pancreas in experimental hypercholesterolemia in normal and in subdiabetic rabbits. Virchows Arch. Abt. A, Path. Anat.353, 99–109 (1971b).
——, Lazarus, S. S.: Ultrastructural pancreatic beta-cell changes in rabbits after small and large doses of alloxan. Diabetes16, 242–251 (1967b).
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Supported by grants from the National Institutes of Health (A-2203) and the National Tay-Sachs and Allied Diseases Association, Inc.
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Adachi, M., Torii, J., Wellmann, K.F. et al. Early central nervous system lesions in experimental hypercholesterolemia in normal and subdiabetic rabbits. Acta Neuropathol 22, 58–67 (1972). https://doi.org/10.1007/BF00687550
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DOI: https://doi.org/10.1007/BF00687550