Summary
One hundred and eighty 12 week old Swiss mice were injected subcutaneously with 0.1 ml suspensions of the E and M variants of encephalomyocarditis (EMC) virus. Ninety-seven surviving mice were sacrificed at intervals from 24 h to 21 days following injection. The M variant group showed only mild microglial proliferation in the brain, while the E variant group displayed various alterations in the astrocytes and neurons exhibiting loss of nuclear membranes and marked changes in the mitochondria and other organelles. In addition, the neuronal cytoplasm displayed aggregates of fine electron-dense granules on the third day which increased in size during the fifth to seventh day. After 7 days, the neurons contained occasional rhomboid structures composed of rows of parallel arranged granules which were similar to those observed in the viscera after EMC virus infection.
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References
Albrecht, P.: Pathogenesis of neurotropic arbovirus infections. Curr. Top. Microbiol. Immunol.43, 44–91 (1968).
Barski, G., Cornefert, F.: Position particulière des encéphalomyélites de type Mengo par rapport aux poliomyélites. Répartition d'anticorps spécifiques chez certaines populations d'Afrique centrale et d'autres zones géographiques. Bull. Wld Hlth Org.17, 991–999 (1957).
Blinzinger, K., Müller, W.: Elektronenmicroskopische Beobachtung des Virus der Frühsommer-Meningoencephalitis (Stamm HYPR) in Nervenzellen von experimentell infizierten Albinomäusen. Dtsch. Z. Nervenheilk.197, 18–27 (1970).
Blinzinger, K., Simon, J., Magrath, D., Boulger, L.: Elektronenmikroskopische Beobachtungen bei experimenteller Poliomyelitis. Experientia (Basel)24, 1095–1096 (1968).
Blinzinger, K., Simon, D., Magrath, D., Boulger, L.: Poliovirus crystals within the endoplasmic reticulum of endothelial and mononuclear cells in monkey spinal cord. Science163, 1336–1337 (1969).
Bodian, D.: Emerging concept of poliomyelitis infection. Science122, 105–108 (1955).
Bodian, D.: Poliovirus in chimpanzee tissues after virus feedings. Amer. J. Hyg.64, 181–197 (1956).
Bodian, D.: An electron-microscopic study of the monkey spinal cord. I. Fine structure of normal motor column. II. Effects of retrograde chromatolysis. III. Cytologic effects of mild and virulent poliovirus infection. Bull. Johns Hopk. Hosp.114, 13–126 (1964).
Burch, G. E., Harb, J. M., Colcolough, H. L., Tsui, C. Y.: Encephalomyocarditis infection of the newborn mouse myocardium: An electron microscopic study. Arch. intern. Med.127, 148–156 (1971a).
Burch, G. E., Tsui, C. Y., Harb, J. M., Colcolough, H. L.: Mural and valvular endocarditis of mice infected with encephalomyocarditis (EMC) virus. Exp. molec. Path.14, 327–336 (1971b).
Burch, G. E., Tsui, C. Y., Harb, J. M.: The early renal lesions of mice infected with encephalomyocarditis virus. Lab. Invest.26, 163–172 (1972).
Coons, A. H., Synder, J. C., Cheever, F. S., Murray, E. S.: Localization of antigen in tissue cells. IV. Antigens of rickettsiae and mumps virus. J. exp. Med.91, 31–38 (1950).
Craighead, J. E., Peralta, P. H., Shelokov, A.: Demonstration of encephalomyocarditis virus antibody in human serums from Panama. Proc. Soc. exp. Biol. (N.Y.)114, 500–503 (1963).
Craighead, J. E.: Pathogenicity of the M and E variants of the encephalomyocarditis (EMC) virus. I. Myocardiotropic and neurotropic properties. Amer. J. Path.48, 333–345 (1966a).
Craighead, J. E.: Pathogenicity of the M and E variants of the encephalomyocarditis (EMC) virus. II. Lesions of the pancreas, parotid and lacrimal glands. Amer. J. Path.48, 375–386 (1966b).
Craighead, J. E., McLane, M. F.: Diabetes mellitus: induction of mice by encephalomyocarditis virus. Science162, 913–914 (1968).
Craighead, J. E., Steinke, J.: Diabetes mellitus-like syndrome in mice infected with encephalomyocarditis virus. Amer. J. Path.63, 119–134 (1971).
Dales, S., Franklin, R. M.: A comparison of the changes in fine structure of L cells during single cycles of viral multiplication, following their infection with the viruses of Mengo and encephalomyocarditis. J. Cell Biol.14, 281–302 (1962).
Friedemann, U.: Permeability of blood-brain barrier to neurotrophic viruses. Arch. Path.35, 912–931 (1943).
Haas, J. E., Yunis, E. J.: Viral crystalline arrays in human Coxsackie myocarditis. Lab. Invest.23, 442–446 (1970).
Helwig, F. C., Schmidt, E. C.: A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science102, 31–33 (1945).
Hurst, E. W.: Newer knowledge of virus diseases of nervous system: Review and interpretation. Brain59, 1–34 (1936).
Johnson, R. T.: Incubation period of viral encephalitis. In: D. C. Gajdusek G. L. Gibbs and M. Alpers (eds.): Slow Latent and Temperate Virus Infection, pp. 119–124. Washington: National Institute of Neurological Diseases and Blindness 1965.
Johnson, R. T., Mims, C. A.: Pathogenesis of viral infections of the nervous system. New Engl. J. Med.278, 23–30 (1968).
Jonkers, A. H.: Serosurvey of encephalomyocarditis virus neutralizing antibodies in southern Louisiana and Peruvian Indian populations. Amer. J. trop. Med. Hyg.10, 593–598 (1961).
Jungeblut, C. W.: Neutralization of Columbia SK and Yale SK virus by polioconvalescent and normal human sera. Arch. Pediat.67, 519–530 (1950).
Kelley, S., Sanderson, W. S.: Comparison of various tissue cultures for the isolation of enteroviruses. Amer. J. publ. Hlth52, 455–459 (1962).
Koch, F.: Die Encephalomyocarditis (EMC) und ihre Abgrenzung von der Poliomyelitis. Z. Kinderheilk.68, 328–357 (1950).
Naoumenko, J., Feigin, I.: A modification for paraffin section of the Cajal gold-sublimate stain for astrocytes. J. Neuropath. exp. Neurol.20, 602–604 (1961).
Naoumenko, J., Feigin, I.: A modification for paraffin sections of the silver carbonate stain for microglia. Acta neuropath. (Berl.)2, 402–406 (1963).
Penman, S., Scherrer, K., Becker, Y., Darnell, J. E.: Polyribosomes in normal and poliovirus-infected Hela cells and their relationship to messenger-RNA. Proc. nat. Acad. Sci. (Wash.)49, 654–662 (1963).
Saphir, O.: Encephalomyocarditis. Circulation6, 843–850 (1952).
Smadel, J. E., Warren, J.: The virus of encephalomyocarditis and its apparent causation of disease in man. J. clin. Invest.26, 1197 (1947).
Sohol, R. S., Burch, G. E., Chu, K. C., Leiderman, E., Colcolough, H. L.: Ultrastructural changes in cardiac capillaries of Coxsackie virus B4-infected mice. Lab. Invest.19, 399–405 (1968).
Warren, J., Smadel, J. E., Russ, S. B.: The family relationship of encephalomyocarditis, Columbia SK, MM, and Mengo encephalomyocarditis viruses. J. Immunol.62, 387–398 (1949).
Wellmann, K. F., Amsterdam, D., Brancato, P., Volk, B. W.: Fine structure of pancreatic islets of mice infected with the M variant of the encephalomyocarditis virus. Diabetologia,8, 349–357 (1972).
Wellmann, K. F., Volk, B. W., Brancato, P., Amsterdam, D.: Encephalomyocarditis virus (E variant) in mice. Pancreatic changes. Arch. Path.95, 304–311 (1973).
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Adachi, M., Volk, B.W., Tsai, C.Y. et al. Light and electron microscopic studies of mouse CNS after subcutaneous administration of the E and M variants of the encephalomyocarditis virus. Acta Neuropathol 25, 169–178 (1973). https://doi.org/10.1007/BF00685196
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DOI: https://doi.org/10.1007/BF00685196