Summary
The uptake of Herpes simplex virus Type I in outgrowing axons of rat spinal cordin vitro was studied by examining the cultures ultrastructurally after inoculation with high titer material. It was found that the virus was taken up both at enlarged axonal regions and along the course of the axons. After attachment of the virus particles to the axons, invaginations of the plasma membrane were formed which were coated on the cytoplasmic side by a filamentous material. This mode of uptake into coated vesicles is similar to the mechanism by which other macromolecular substances, such as protein tracers, may by incorporated into axons bothin vitro andin vivo. Whether the viruses taken up in this way are infectious to the neuron could be determined from this morphological study.
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Kristensson, K., Sheppard, R.D. & Bornstein, M.B. Observations on uptake of Herpes simplex virus in organized cultures of mammalian nervous tissue. Acta Neuropathol 28, 37–44 (1974). https://doi.org/10.1007/BF00687516
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DOI: https://doi.org/10.1007/BF00687516