Summary
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1.
The passage of fluorescein labeled as well as radioactive I131 labeled albumin and gamma globulin into the choroid plexus of the chick and rabbit was studiedin vitro.
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2.
Incubations in solutions of fluorescent tracers [fluorescein labeled albumin (FLA), fluorescein labeled gamma globulin (FLGG), and Strophanthin K] revealed two patterns of uptake by the isolated chick choroid plexus characterized by either predominant stromal or epithelial uptake.
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3.
At room temperature FLA and Strophanthin K were shown to penetrate rapidly into the stroma without any visible accumulation of these tracers in the choroidal epithelium. This stromal passage revealed features compatible with active transport, i.e. effect of low temperature and of metabolic inhibitors.
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4.
The capacity for the stromal uptake of FLA by the chick choroid plexus was shown to develop between the 9th and 13th days of embryonic life. No penetration of FLGG into the chick choroid plexus was observed at any stage.
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5.
The incubations in fluorescent proteins and Strophanthin K with addition of unlabeled gamma globulin resulted in intense uptake of these tracers by the choroidal epithelium. No such effect was achieved in incubations with addition of unlabeled albumin.
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6.
The observations on the uptake of radioactive albumin and gamma globulin by the rabbit choroid plexus revealed that radioactive iodinated human serum albumin (RIHSA) uptake was inhibited by low temperature and by metabolic inhibitors whereas the uptake of radioactive iodinated human serum gamma globulin (RIHSGG) was reduced by low temperature only.
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7.
The significance of these observations particularly with reference to the possible role of active transport and pinocytosis is discussed.
Zusammenfassung
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1.
Der Durchtritt von mit Fluorescein sowie mit radioaktiv-I131 markiertem Albumin und Gamma-Globulin durch den Plexus chorioideus des Huhns und des Kaninchens wurdein vitro studiert.
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2.
Die Inkubation in Lösungen von fluorescenzmarkierten Substanzen (FLA, FLGG und Strophanthin K) brachte zwei Aufnahmeweisen im isolierten Plexus chorioideus des Huhns zum Vorschein, und zwar eine überwiegend stromale und eine überwiegend epitheliale Aufnahme.
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3.
Bei Raumtemperatur zeigten FLA und Strophanthin K ein rasches Eindringen in das Stroma ohne sichtbare Anhäufung im Plexusepithel. Diese Stromapassagen zeigten Eigenschaften, die mit dem aktiven Transport vergleichbar sind, d.h. der Einfluß niedriger Temperaturen und metabolischer Hemmsubstanzen.
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4.
Die Fähigkeit zur stromalen Aufnahme von FLA des Plexus chorioideus des Huhns tritt zwischen dem 9.—13. Tag des Embryonallebens auf. In keinem Stadium wurde ein Eindringen von FLGG in den Plexus chorioideus des Huhns beobachtet.
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5.
Die Inkubation von fluorescierenden Proteinen und Strophanthin K mit Zusatz von unmarkiertem Gamma-Globulin führte zu einer intensiven Aufnahme dieser Substanzen durch das Plexusepithel. Ein ähnlicher Effekt konnte bei Inkubation mit Zusatz von unmarkiertem Albumin nicht erzeugt werden.
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6.
Die Beobachtungen über die Aufnahme von radioaktivem Albumin und Gamma-Globulin durch den Plexus chorioideus des Kaninchens zeigten, daß die RIHSA-Aufnahme durch niedrige Temperaturen und durch metabolische Hemmstoffe verhindert wurde, während im Gegensatz hierzu die RIHSGG-Aufnahme durch niedrige Temperaturen lediglich herabgesetzt wurde.
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7.
Die Bedeutung dieser Beobachtungen wird besonders im Hinblick auf die mögliche Rolle des aktiven Transportes und der Pinocytose diskutiert.
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Smith, D.E., Streicher, E., Milković, K. et al. Observations on the transport of proteins by the isolated choroid plexus. Acta Neuropathol 3, 372–386 (1964). https://doi.org/10.1007/BF00691845
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DOI: https://doi.org/10.1007/BF00691845