Summary
Six clones from methylnitrosourea (MNU) or ethylnitrosourea (ENU) induced tumours obtained in the nervous system of the rat were cultured in serum-free medium or treated with dibutyryl cyclic AMP (db cAMP) in vitro. All clones originated from longterm cultures. Three clones forming sarcomas after syngeneic transplantation showed only very slight changes following treatment, whereas the three glioma clones showed striking alterations. They formed long processes or showed rounding of their perikarya. In serum-free medium the cellular shape is intermediate between that seen in normal conditions and that seen in db cAMP treated cultures. The altered cultures resemble the primary cultures of the respective tumours. The relationship of these alterations to tumour types are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Donta, S. T.: The growth of functional rat glial cells in a serumless medium. Exp. Cell Res82, 119–124 (1973)
Gibson, D. A., Reichlin, S., Vernadakis, A.: (3H) Uridine uptake and incorporation into RNA in the C-6 glial cells following dibutyryl cyclic AMP treatment. Brain Res.81, 354–360 (1974)
Hsie, A. W., Puck, T. T.: Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3′,5′ monophosphate and testosterone. Proc. nat. Acad. Sci. (Wash.)68, 358–361 (1971)
Johnson, G. S., Friedman, R. M., Pastan, I. Restoration of several morphological characteristics of normal fibroblasts in sarcoma cells treated with adenosine-3′,5′-cyclic monophosphate and its derivatives. Proc. nat. Acad. Sci. (Wash.)68, 425–429 (1971)
Kersting, G.: Die Gewebszüchtung menschlicher Hirngeschwülste. Monogr. Gesamtgeb. Neurol. Psychiatr.90, Berlin-Göttingen-Heidelberg: Springer 1961
Kurth, R., Bauer, H.: Correlation between intracellular cyclic AMP level and cell membrane antigenicity of normal and malignant cells. Differentiation1, 323–330 (1973)
Porter, K. R., Puck, T. T., Hsie, A. W., Kelley, D.: An electron microscope study of the effects of dibutyryl cyclic AMP on Chinese Hamster ovary cells. Cell2, 145–162 (1974)
Robinson, G. A., Butcher, R. W., Sutherland, E. W.: Cyclic AMP. New York-London: Academic Press 1971
Schubert, D., Heinemann, S., Carlisle, W., Tarikas, H., Kimes, B., Patrick, J., Steinbach, J. H., Culp, W., Brandt, B. L.: Clonal cell lines from the rat central nervous system. Nature (Lond.)249, 224–227 (1974)
Sheppard, J. R.: The role of cyclic AMP in the control of cell division. In: Cyclic AMP, cell growth and the immune response, (eds. W. Braun, L. M. Lichtenstein, C. W. Parker) pp. 290–301. Berlin-Heidelberg-New York: Springer 1974
Thust, R.: Karyotypveränderungen in Klonen eines N-Methylnitrosoharnstoff-induzierten euploiden Sarkoms des Rückenmarks der Ratte in vitro. Exp. Path.5, 226–234 (1971)
Thust, R.: Morphologie und Zytogenetik experimenteller Tumoren des Nervensystems in vitro. Promotion B (Jena) (1975)
Thust, R., Warzok, R.: Comparative studies on the in vitro and in vivo morphology of clones of experimental CNS tumours in the rat. Acta neuropath. (Berl.)20, 248–257 (1972)
Thust, R., Warzok, R.: The morphology of experimental tumours of the nervous system in vitro. II. Intracerebral tumours in the rat. Acta neuropath. (Berl.)27, 25–32 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thust, R., Warzok, R. Differential morphological reaction of experimental CNS tumour clonesin vitro to dibutyryl cyclic AMP or serum-free medium, resp.. Acta Neuropathol 33, 325–332 (1975). https://doi.org/10.1007/BF00686164
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00686164