Summary
Sixteen 3 month old “nude” mice, 24 of their litter mates and 30 Swiss mice were injected subcutaneously with 0.1 ml suspension of the E variant of the encephalomyocarditis (EMC) virus. While the mortality rate of the litter mates and Swiss mice during 5–7 days after inoculation was more than 40%, none of the “nude” mice died during the experiment. The surviving animals were sacrificed at 24 h intervals from day one to seven days after injection. Brain suspensions assayed for the presence of the virus yielded significant titers at 24 h in all groups, which increased during 7 days. The litter mates and Swiss mice showed proliferation of lymphocytes and microglial cells in the perivascular areas of the brain during the fifth to the seventh day. The “nude” mice, on the other hand, displayed no perivascular lymphocytic infiltration during the same periods. Ultrastructurally, all groups showed aggregates of ribosomes in the cytoplasmic matrix on the third day, which became enlarged in size on the 5th day. At 7 days, both litter mates and Swiss mice showed an increased number of necrotic cells, while these changes were not observed in the “nude” mice. These findings suggest that the high mortality rate in immunologically normal mice was related to the efforts of T cells to eliminate virus-infected cells and to produce extensive necrosis, while T cell-depleted animals showed good survival rates.
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Adachi, M., Volk, B. W., Tsai, C.-Y., Amsterdam, D., Wellmann, K. F.: Light and electron microscopic studies of mouse CNS after subcutaneous administration of the E and M variants of the encephalomyocarditis virus. Acta neuropath. (Berl.)25, 169–178 (1973)
Adachi, M., Amsterdam, D., Brooks, S. E., Volk, B. W.: Ultrastructural alterations of tissue cultures from human fetal brain infected with the E variant of EMC virus. Acta neuropath. (Berl.)32, 133–142 (1975)
Bergsma, D. (Editor): Immunodeficiency in man and animals, National Foundation March of Dimes XI. Sunderland, Mass.: Sinauer Assoc. 1975
Cole, G. A., Nathanson, N., Prendergast, R. A.: Requirement for θ-bearing cells in lymphocytic choriomeningitis virus-induced central nervous system disease. Nature (Lond.)238, 335–337 (1972)
Hirsch, M. S., Murphy, F. A.: Effects of anti-thymocyte serum on 17-D yellow fever infection in adult mice. Nature216, 179–180 (1967)
Hotchin, J. H.: Persistent and slow virus infections. Monogr. Virol.3 (1971)
Isaacson, J. H., Cattanach, B. M.: Report. Mouse News Letter27, 31 (1962)
Martinex, C., Kersey, J., Papermaster, B. W., Good, R. A.: Skin homograft survival in thymectomized mice. Proc. Soc. exp. Biol. (N. Y.)109, 193–196 (1962)
Miller, J. F. A. P.: Immunologic function of the thymus. Lancet. 21961, 748–749
Naoumenko, J., Feigin, I.: A modification for paraffin section of the Cajal gold-sublimate stain for astrocytes. J. Neuropath. exp. Neurol.20, 602–604 (1961)
Naoumenko, J., Feigin, I.: A modification for paraffin sections of the silver carbonate stain for microglia. Acta neuropath. (Berl.)2, 402–406 (1963)
Pantelouris, E. M.: Absence of thymus in a mouse mutant. Nature (Lond.)217, 370–371 (1968)
Rygaard, J.: Immunobiology of the mouse mutant “Nude”. Acta path. microbiol. scand.77, 761–762 (1969)
Rygaard, J., Povlsen, C. O.: Heterotransplantation of a human malignant tumour to “Nude” mice. Acta path. microbiol. scand.77, 758–760 (1969)
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Adachi, M., Volk, B.W., Amsterdam, D. et al. Light and electron microscopic studies of “nude” mice CNS after subcutaneous administration of the E variant of the encephalomyocarditis (EMC) virus. Acta Neuropathol 37, 89–93 (1977). https://doi.org/10.1007/BF00692053
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DOI: https://doi.org/10.1007/BF00692053