Summary
By means of the explant technique a series of primary tumours induced by methylnitrosourea (MNU) or ethylnitrosourea (ENU) in the brain of rats were cultured in vitro. The morphology of isomorphic oligodendrogliomas, astrocytomas, glioblastomas and sarcomas under in vitro conditions is described. All these neoplasias show a very distinct morphological picture and growth behaviour, thus permitting their classification. Most of the tumours tend to anaplastic changes during longer cultivation, but in some cases, preferably in well differentiated gliomas, the cytological properties are maintained even during longterm cultivation. The role of anaplasia and intermingling of different neoplastic cell types in experimental tumours are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benda, P., Lightbody, J., Sato, G., Levine, L., Sweet, W.: Differentiated rat glial str tissue culture. Science161, 370 (1968)
Benda, P., Someda, K., Messer, J., Sweet, W.: Morphological and immunochemical studies of rat glial tumors and clonal strains propagated in culture. J. Neurosurg.34, 310–323 (1971)
Cravioto, H., Palekar, L., Weiss, E., Bennett, K.: Experimental neurinoma in tissue culture. Acta neuropath. (Berl.)21, 154–164 (1972)
Davidson, R. L., Benda, P.: Regulation of specific functions of glial cells in somatic hybrids. II. Control of inducibility of glycerol-3-phosphate dehydrogenase. Proc. nat. Acad. Sci. (Wash.)67, 1870–1877 (1970)
Dorfman, A., Ho, P. L.: Synthesis of acid mucopolysaccharides by glial tumor cells in tissue culture. Proc. nat. Acad. Sci. (Wash.)66, 495–499 (1970)
Fornatto, L., Schiffer, D.: In vitro culture observations on neurinoma induced experimentally in the rat by ethylnitrosourea. Acta neuropath. (Berl.)20, 199–206 (1972)
Grinyo, A.: Staining of oligodendroglia with silver amino tungstate in unembedded and embedded material. Stain Technol.43, 19–25 (1968)
Janzen, H. W., Millman, P. A., Thurston, O. G.: Hybrid cells in solid tumors. Cancer27, 455–459 (1971)
Jänisch, W., Schreiber, D.: Experimentelle Geschwülste des Zentralnervensystems. Induktion, Morphologie, Transplantation und Anwendung. Jena: Fischer 1969
Kersting, G.: Die Gewebszüchtung menschlicher Hirngeschwülste. Monogr. Gesamtgeb. Neurol. Psychiat., Bd. 90. Berlin-Göttingen-Heidelberg: Springer 1961
Koestner, A., Swenberg, J. A., Wechsler, W.: Experimental tumors of the nervous system induced by resorptive N-nitrosourea compounds. Progr. exp. Tumor Res.17, 9–30 (1972)
Lumsden, C. E.: Tissue culture in relation to tumours of the nervous system. In: D. S. Russell and L. J. Rubinstein: Pathology of tumours of the nervous system. London: Arnold 1963
Renkawek, K., Kroh, H.: The morphological and histoenzymatic properties of experimental brain sarcomas cultured in vitro. Z. Krebsforsch.75, 123–132 (1971)
Renkawek, K., Kroh, H., Krásnicka, Z.: Experimental gliomas cultured in vitro. Morphological and histochemical study. Z. Krebsforsch.77, 247–256 (1972)
Silbert, S. W., Goldstein, M. N.: Drug-induced differentiation of a rat glioma in vitro. Cancer Res.32, 1422–1427 (1972)
Stavrou, D.: Beitrag zur Morphologie und Enzymhistochemie experimenteller Tumoren des Zentralnervensystems der Ratte. I. Experimentelle Befunde. Acta neuropath. (Berl.)15, 220–230 (1970)
Stavrou, D., Schiefer, B., Swoboda, R.: Zur Frage des “Grading” von Gliomen bei Tieren. Beitr. path. Anat.140, 182–193 (1970)
Thust, R.: Klone monstrocellulärer experimenteller Tumoren des Zentralnervensystems des Hundes und der Ratte in vitro. Exp. Path.6, 158–166 (1972)
Thust, R., Warzok, R.: Comparative studies on the in vitro and in vivo morphology of clones of experimental tumours in the rat. Acta neuropath. (Berl.)20, 248–257 (1972)
Thust, R., Warzok, R., Batka, H.: Die Morphologie experimenteller Tumoren des Nervensystems in vitro. I. Transplazentar induzierte Tumoren des Nervus trigeminus der Ratte. Arch. Geschwulstforsch.40, 300–307 (1972)
Thust, R., Warzok, R., Osske, G.: Untersuchungen zur Histogenese experimenteller Hirngeschwülste. 2. Mitt.: Zytologische Befunde an Zellkulturen. Exp. Path.5, 217–225 (1971)
Zimmerman, H. M.: Brain tumors: Their incidence and classification in man and their experimental production. Ann. N.Y. Acad. Sci.159, 337–359 (1969)
Zülch, K. J.: The newest development of experimental induced tumors of the central nervous system. J. Génét. hum.17, 511–527 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thust, R., Warzok, R. The morphology of experimental tumours of the nervous system. Acta Neuropathol 27, 25–32 (1974). https://doi.org/10.1007/BF00687237
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00687237