Summary
Thirty two human tumours, mainly neurogenic, have been investigated for the presence of adenovirus-related RNA sequences.3H-labelled tumour virus DNA probes derived from human adenoviruses types 2 and 12, bovine adenovirus type 3, and avian adenovirus CELO were hybridized in-situ on tumour kryostat sections under conditions that detect complementary RNA. Tumour virus-related RNA was detected in 62% of all tumours tested, but was not detectable in normal human brain tissues. Expression of tumour virus-related RNA was found in 2/4 astrocytomas, 2/4 metastatic brain carcinomas, 2/2 glioblastomas, 1/1 melanoma, 5/7 meningiomas, 4/4 neurinomas, 1/2 oligodendrogliomas, and 1/1 rhabdomyosarcoma. The presence of adenovirus-related RNA in the majority of human neurogenic tumours may reflect a viral involvement in the pathogenesis of these tumours.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cooper JR (1967) The growth of tumour tissue of the central nervous system in tissue culture. J Kansas Med Soc 68:340–343
Godard C, Jones KW (1979) Detection of AKR Mu LV-specific RNA in AKR mous cells by in situ hybridization. Nucl Acid Res 6:2849–2862
Godard C, Jones KW (1980) Improved method for detection of cellular transcripts by in situ hybridization: detection of poly(A) sequences in individual cells. Histochemistry 65:291–300
Green M, Wold WSM, Mackey JK, Rigden P (1979) Analysis of human tonsil and cancer DNAs and RNAs for DNA sequences of group C (serotypes 1, 2, 5, 6) human adenoviruses. Proc Soc Acad Sci USA 76:6606–6610
Green M, Wold WSM, Brackmann KH, Cartas MA, Sanders PR, Olson K, Lee TK, Young L, Matsuo T, Kapoor Q (1980) Human adenovirus transforming genes: group relationships, integration, expression in transformed cells, and analyses of human cancers and tonsils. In: Essex M, Todaro G, zur Hausen H (eds) 7th Cold Spring Harbor Conferences on Cell Proliferation: Viruses in naturally occurring tumours. Cold Spring Harbor Laboratory, New York, pp 373–397
Ibelgaufts H, Doerfler W, Scheidtmann KH, Wechsler W (1980) Adenovirus type 12-induced rat tumour cells of neuroepithelial origin: persistence and expression of the viral genome. J Virol 33:423–437
Ibelgaufts H (1982) Are human DNA tumour viruses involved in the pathogenesis of human neurogenic tumours? Neurosurg Rev 5: (in press)
Larson VM, Gosnell PA, Hilleman MR (1967) Complement-fixation reaction of human neoplastic tissue with sera from hamsters bearing virus-induced tumours. Proc Soc Exp Biol Med 125:525–534
Lord MA, Itshaki RF, Sutton RNP (1980) Detection of virus genome in human tissues. Lancet 2:92
McAllister RM, Landing BH, Goodheart CR (1964) Isolation of adenoviruses from neoplastic and non-neoplastic tissues of children. Lab Invest 13:894–901
McAllister RM, Gilden RV, Green M (1972) Adenoviruses in human cancer. Lancet 1:831–833
McCormick KJ, Van Hoosier GL, Trentin JJ (1968) Attempts to find human adenovirus type 12 tumour antigens in human tumours. J Natl Cancer Inst 40:255–261
Mackey JK, Rigden PM, Green M (1976) Do highly oncogenic group A human adenoviruses cause human cancer? Analysis of human tumours for adenovirus 12 transforming DNA sequences. Proc Natl Acad Sci USA 73:4657–4661
Mackey JK, Green M, Wold WSM, Rigden P (1979) Analysis of human cancer DNA for DNA sequences of human adenovirus type 4. J Natl Cancer Inst 62:23–26
Mukai N (1976) Human adenovirus-induced embryonic neuronal tumour phenotype in rodents. In: Zimmerman H (ed) Progress in neuropathology, vol III. Grune and Stratton, New York
Mukai N, Kalter SS, Cummins LB, Matthews VA, Nishida T, Nakajima T (1980) Retinal tumours induced in the baboon by human adenovirus 12. Science 210:1023–1025
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Wold WSM, Mackey JK, Rigden P, Green M (1979) Analysis of human cancer DNAs for DNA sequences of human adenovirus serotypes 3, 7, 11, 14, 16, and 21 in group B. Cancer Res 39:3479–3484
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ibelgaufts, H., Jones, K.W., Maitland, N. et al. Adenovirus-related RNA sequences in human neurogenic tumors. Acta Neuropathol 56, 113–117 (1982). https://doi.org/10.1007/BF00690581
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00690581