Skip to main content
SpringerLink
Log in

Immunofluorescent staining of frozen sections for glial fibrillary acidic protein

  • Short Original Communications
  • Published:
Acta Neuropathologica Aims and scope Submit manuscript

Summary

Immunofluorescent staining for glial fibrillary acidic protein (GFAP) has been used as part of the diagnostic evaluation of eleven patients and compared with routine special stains. In one case, a difficult fibrillary neoplasm of the spinal cord, this diagnostic procedure provided rapid, positive identification of the glial nature of the tumor. In all cases, the GFAP reactivity was consistent with staining properties of PTAH and more rapid than PTAH.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bignami A, Eng LF, Dahl D, Uyeda CT (1972) Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 43:429–435

    Google Scholar 

  2. Bowling MC (1967) Histopathology laboratory procedures of the pathologic anatomy branch of the National Cancer Institute. H.E.W. Publication no. 1595. U.S. Govt. Printing Office, Washington, DC, pp 1–125

    Google Scholar 

  3. Burger PC, Vogel FS (1976) Surgical pathology of the nervous system and its coverings. Wiley, New York, pp 1–623

    Google Scholar 

  4. Coons AH (1958) Fluorescent antibody methods. In: Danielli JF (ed) General cytochemical methods, vol I. Academic Press, New York, pp 399–422

    Google Scholar 

  5. Deck JH, Eng LF, Bigbee J (1976) A preliminary study of glioma morphology using the peroxidase-antiperoxidase method for the GFA protein. J Neuropathol Exp Neurol 35:362

    Google Scholar 

  6. Delpech B, Delpech A, Vidard MN, Girard N, Tayot J, Element JC, Creissard P (1978) Glial fibrillary acidic protein in tumours of the nervous system. Br J Cancer 37:33–40

    Google Scholar 

  7. Duffy PE, Graf L, Raport MN (1977) Identification of glial fibrillary acidic protein by the immunoperoxidase method in human brain tumors. J Neuropathol Exp Neurol 36:645–652

    Google Scholar 

  8. Eng LF, Rubinstein LJ (1978) Contribution of immunohistochemistry to diagnostic problems of human cerebral tumors. J Histochem Cytochem 26:513–522

    Google Scholar 

  9. Gambetti P, Roessman U, Velasco ME (1980) Immunofluorescence technique for rapid diagnosis of glial tumors. Am J Surg Pathol 4:277–280

    Google Scholar 

  10. Kepes JJ (1971) Differential diagnostic problems of brain tumors. In: Minckler J (ed) Pathology of the nervous system, vol 2. McGraw-Hill, New York, pp 2219–2237

    Google Scholar 

  11. Kepes JJ, Morantz RA, England AM (1979) Reticulin stain in differentiating astrocytomas from neurilemmonas on frozen sections. J Neurosurg 51:124–125

    Google Scholar 

  12. Laverson S, McKeever PE, Kornblith PL, Quindlen EA, Howard R (1981) Diagnosis of glioma on frozen section by immunofluorescence for glial fibrillary acidic protein. Lancet 1:674

    Google Scholar 

  13. McKeever PE, Brissie NT (1977) Scanning electron microscopy of neoplasms removed at surgery: Surface topography and comparison of meningioma, colloid cyst, ependymoma, pituitary adenoma, schwannoma and astrocytoma. J Neuropathol Exp Neurol 36:875–896

    Google Scholar 

  14. McKeever PE, Garvin AJ, Spicer SS (1976) Immune complex receptors on cell surfaces. I. Ultrastructural demonstration on macrophages. J Histochem Cytochem 24:948–955, 1212

    Google Scholar 

  15. Van der Meulen JDM, Houthoff HJ, Ebels EJ (1978) Glial fibrillary acidic protein in human gliomas. Neuropathol Appl Neurobiol 4:177–190

    Google Scholar 

  16. Velasco ME, Dahl D, Roessman U, Gambetti P (1980) Immunohistochemical localization of glial fibrillary acidic protein in human glial neoplasms. Cancer 45:484–494

    Google Scholar 

  17. Velasco ME, Sindely SO, Roessman N (1977) Reticulum stain for frozen-section diagnosis of pituitary adenomas. J Neurosurg 46:548–550

    Google Scholar 

  18. Vraa-Jensen J, Herman MM, Rubinstein LJ, Bignami A (1976) In vitro characteristics of a fourth ventricle ependymoma maintained in organ culture systems: Light and electron microscopic observations. Neuropathol Appl Neurobiol 2:349–364

    Google Scholar 

  19. Wilde HC (1935) An improved technique for silver impregnation of reticulum fibers. Am J Pathol 11:817–819

    Google Scholar 

  20. Yen SH, Fields KL (1981) Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous system. J Cell Biol 88:115–126

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Surgical Neurology Branch, National Institute of Neurological and Communicative Disorders and Stroke, 20205, Bethesda, MD, USA

    P. E. McKeever, S. Laverson, P. L. Kornblith, R. Howard, E. A. Quindlen, B. H. Smith & B. M. Chronwall

  2. Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 20205, Bethesda, MD, USA

    P. E. McKeever, S. Laverson, P. L. Kornblith, R. Howard, E. A. Quindlen, B. H. Smith & B. M. Chronwall

Authors
  1. P. E. McKeever
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Laverson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. L. Kornblith
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Howard
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. A. Quindlen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. H. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. B. M. Chronwall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

McKeever, P.E., Laverson, S., Kornblith, P.L. et al. Immunofluorescent staining of frozen sections for glial fibrillary acidic protein. Acta Neuropathol 58, 69–72 (1982). https://doi.org/10.1007/BF00692700

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00692700

Key words

Search

Navigation